A5052250296- RNA Research and Splicing
- Heat shock proteins research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Signaling Pathways in Disease
- Calpain Protease Function and Regulation
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Electrospun Nanofibers in Biomedical Applications
- Metabolomics and Mass Spectrometry Studies
- Phagocytosis and Immune Regulation
- Hereditary Neurological Disorders
- Muscle Physiology and Disorders
- Renal and related cancers
- Cancer-related gene regulation
- Cellular transport and secretion
- Zebrafish Biomedical Research Applications
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Melanoma and MAPK Pathways
University of Otago
2011-2025
Maurice Wilkins Centre
2020-2024
Genesis Laboratories
2011
University of Auckland
2011
Wake Forest University
2011
Children's Medical Research Institute
2011
The University of Sydney
2011
Asbestos Diseases Research Institute
2011
CSL (Australia)
2011
Y-box binding factor 1 (YB-1) has been associated with prognosis in many tumor types. Reduced YB-1 expression inhibits cell growth, but the mechanism is unclear. mRNA levels were compared grade and histology using microarray data from 771 breast cancer patients disease-free survival distant metastasis–free 375 of those who did not receive adjuvant therapy. Microarrays further searched for genes that had correlated mRNA. Small interfering RNA (siRNA) was used to study effects reduced on...
Background We are investigating the molecular basis of melanoma by defining genomic characteristics that correlate with tumour phenotype in a novel panel metastatic cell lines. The aim this study is to identify new prognostic markers and therapeutic targets might aid clinical cancer diagnosis management. Principal Findings Global transcript profiling identified signature featuring decreased expression developmental lineage specification genes including MITF, EDNRB, DCT, TYR, increased...
Chemotherapy with taxanes such as paclitaxel (PTX) is a key component of triple negative breast cancer (TNBC) treatment. PTX used in combination other drugs both the adjuvant setting and advanced cancer. Because proportion patients respond poorly to or relapse after its use, greater understanding mechanisms conferring resistance required. One protein shown be involved drug Y-box binding 1 (YB-1). High levels YB-1 have previously been associated TNBCs. In this study, we aimed determine by...
The literature concerning the subcellular location of Y-box binding protein 1 (YB-1), its abundance in normal and cancer tissues, prognostic significance is replete with inconsistencies. An explanation for this could be due part to use different antibodies immunohistochemical immunofluorescent labeling cells tissues. inconsistencies also poor resolution data. We analyzed two cohorts breast tumours both YB-1 using three antibodies; targeting N-terminal epitopes (AB-a AB-b) another (AB-c) a...
<title>Abstract</title> Mutations in the tumor suppressor, <italic>TP53</italic>, are prevalent human cancers; yet their functional implications remain unclear. Through comprehensive pan-cancer database analysis, we identified H179 mutations <italic>TP53</italic> as significantly associated with poor disease-free survival across multiple cancer types. Functional studies lung, ovarian, and prostate cells overexpressing p53<sup>H179R/Y</sup> mutants revealed that these not only defective...
GLI pathogenesis-related 1 (GLIPR1) was previously identified as an epigenetically regulated tumor suppressor in prostate cancer and, conversely, oncoprotein glioma. More recently, GLIPR1 shown to be differentially expressed other cancers including ovarian, acute myeloid leukemia, and Wilms' tumor. Here we investigated expression metastatic melanoma cell lines tissue. variably cells, transcript levels correlated with degree of promoter methylation vitro. Elevated were increased invasive...
Elevated levels of nuclear Y-box binding protein 1 (YB-1) are linked to poor prognosis in cancer. It has been proposed that entry into the nucleus requires specific proteasomal cleavage. However, evidence for cleavage is contradictory and high YB-1 prognostic regardless cellular location. Here, using confocal microscopy mass spectrometry, we find no proteolytic Doxorubicin treatment, resultant G2 arrest, leads a significant increase number cells where not found cytoplasm, suggesting its...
Abstract This work investigates the role of NT‐3 in peripheral myelination. Recent articles, based vitro , propose that acting through its high‐affinity receptor TrkC may act to inhibit myelin formation by enhancing Schwann cell motility and/or migration. Here, we investigate this hypothesis vivo examining myelination mutant mice. On day birth, soon after onset myelination, axons showed normal ensheathment cells, no change proportion which had begun myelinate, and either thickness or number...
Abstract When a tooth is diseased or damaged through caries, bioactive molecules are liberated from the pulp and dentin as part of natural response to injury these key for stimulating stem cell responses tissue repair. Incorporation extracellular‐matrix (ECM)‐derived into hydrogel model can mimic in vivo conditions enable dentin–pulp complex regeneration. Here, chitosan/alginate (C/A) developed sequester bovine ECM extracts. Human dental cells (hDPCs) cultured with constructs proliferation...
Abstract This paper examines early postnatal development of the neuromuscular system in mice with a null mutation gene for neurotrophin‐3. We report that alpha‐motoneurons at first develop substantially normally, despite known 15% deficit their somal size [ Woolley et al . (1999) Neurosci. Lett. , 272, 107–110.] and absence proprioceptive input Ernfors (1994) Cell 77, 503–512]. At birth, motor axons have extended into muscle, forming normal‐looking junctions focal accumulations acetylcholine...
Abstract Neurotrophin‐3 (NT‐3) is a trophic factor that essential for the normal development and maintenance of proprioceptive sensory neurons widely implicated as an important modulator synaptic function development. We have previously found animals lacking NT‐3 number structural abnormalities in peripheral nerves skeletal muscles. Here we investigated whether haploinsufficiency‐induced reduction resulted impaired neuromuscular performance function. Motor nerve terminal was tested by...
High levels of the cold shock protein Y-box-binding protein-1, YB-1, are tightly correlated with increased cell proliferation and progression. However, precise mechanism by which YB-1 regulates is unknown. Here, we found that depletion in several cancer lines immortalized fibroblasts resulted cytokinesis failure consequent multinucleation. Rescue experiments indicated was required for completion cytokinesis. Using confocal imaging essential orchestrating spatio-temporal distribution...
Abstract Background The p53 isoform Δ133p53β is known to be associated with cancers driven by inflammation. Many of the features development inflammation in rheumatoid arthritis (RA) parallel those evident cancer progression. However, role this RA has not yet been explored. aim study was determine whether driving aggressive disease RA. Methods Using patient synovia, we carried out RT-qPCR and RNAScope-ISH both protein mRNA levels Δ133p53 p53. We also used IHC location type cells elevated...
ABSTRACT High levels of the cold shock protein Y-box-binding protein-1, YB-1, are tightly correlated with increased cell proliferation and cancer progression. However, precise mechanism by which YB-1 regulates is unknown. Here, we found that depletion in several lines resulted cytokinesis failure, multinucleation an increase G 1 transit time. Rescue experiments indicated was required for completion cytokinesis. Using confocal imaging cells undergoing both vitro zebrafish embryos, critical...
Abstract Introduction The filamins are cytoskeletal binding proteins that dynamically crosslink actin into orthogonal networks or bundle it stress fibres. domain structure of filamin is very well characterised, with an N‐terminal actin‐binding region, followed by 24 immunoglobulin‐like repeat units. domains separated distinct segments two regions low‐complexity known as hinge‐1 and hinge‐2. role especially has been proposed to be essential for protein function provides flexibility the...
Abstract The initiation of CD8+ T cell responses against dead cell–associated Ags is tightly regulated, facilitating adaptive immunity pathogens and tumors while preventing autoimmunity. It now well established that dying cells actively regulate the generation via release or exposure damage-associated molecular patterns. However, it unclear whether nonproteasomal proteases (activated in stressed cells) can influence availability for cross-presentation. Using a mouse model immunogenic...
Colorectal cancer is primarily a disease of the developed world. The incidence rate has continued to increase over time, reflecting both demographic and lifestyle changes, which have resulted in genomic epigenomic modifications. Many epigenetic modifications occur genes known be closely associated with embryonic development cellular growth. In particular, paired box (PAX) transcription factors are crucial for correct tissue during embryogenesis due their role regulating involved...