A5016682435- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Acute Kidney Injury Research
- Mitochondrial Function and Pathology
- Chromosomal and Genetic Variations
- Sepsis Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Cardiomyopathy and Myosin Studies
- Pancreatic function and diabetes
- Reconstructive Facial Surgery Techniques
- Parvovirus B19 Infection Studies
- Medical Coding and Health Information
- Cellular transport and secretion
- Genomic variations and chromosomal abnormalities
- Traumatic Ocular and Foreign Body Injuries
- Regulation of Appetite and Obesity
- Medical Research and Treatments
- Photosynthetic Processes and Mechanisms
- Ear and Head Tumors
- Hemophilia Treatment and Research
- Muscle Physiology and Disorders
- Genomics and Chromatin Dynamics
Fudan University
2005-2025
Chengdu Military General Hospital
2023
Yale University
2007-2023
Huazhong University of Science and Technology
2022
Central Hospital of Wuhan
2022
Plastic Surgery Hospital
2017-2020
Howard Hughes Medical Institute
2015-2019
Qingdao Agricultural University
2017
Children's Hospital of Fudan University
2016
Amunix (United States)
2014-2015
Abstract Endometriosis is an estrogen-dependent inflammatory disorder among reproductive-aged women associated with pelvic pain, anxiety, and depression. Pain characterized by central sensitization; however, it not clear if endometriosis leads to increased pain perception or the disease are more sensitive increasing detection of endometriosis. was induced in mice changes behavior including perception, brain electrophysiology, gene expression were characterized. Behavioral tests revealed that...
Serum phosphate levels are strongly correlated with the prognosis of septic patients. However, previous studies have concentrated on individual levels, and relationship between change trends in serum in-hospital mortality has seldom been reported. We aimed to investigate whether level were associated mortality. classified patients using k-means clustering analysis into clusters changes used logistic regressions explore relationships different mortality, taking cluster smallest as a...
Palatable foods (fat and sweet) induce hyperphagia, facilitate the development of obesity. Whether how overnutrition increases appetite through adipose-to-brain axis is unclear. O-linked beta-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) couples nutrient cues to O-GlcNAcylation intracellular proteins at serine/threonine residues. Chronic dysregulation O-GlcNAc signaling contributes metabolic diseases. Here we show that adipocyte OGT essential for high fat diet-induced but dispensable...
XTENs are unstructured, nonrepetitive protein polymers designed to prolong the in vivo half-life of pharmaceuticals by introducing a bulking effect similar that poly(ethylene glycol). While XTEN can be expressed as recombinant fusion with bioactive proteins and peptides, therapeutic molecules interest also chemically conjugated XTEN. Such an approach permits precise control over positioning, spacing, valency moieties along length We have demonstrated attachment T-20, anti-retroviral peptide...
Single-nucleus RNA sequencing (snRNA-seq) has emerged as a powerful approach for studying cellular heterogeneity in metabolic tissues. However, snRNA-seq analysis remains challenging due to low gene expression and data complexity. Here, we introduce an optimized upstream analytical workflow from 67 samples across white adipose tissue, muscle, liver the hypothalamus. We emphasized importance of key steps including ambient removal, doublet identification integration ensure accurate downstream...
DNA transposon piggyBac (PB) is a newly established mutagen for large-scale mutagenesis in mice. We have designed and implemented an integrated database system called PBmice (PB Mutagenesis Information CEnter) storing, retrieving displaying the information derived from PB insertions (INSERTs) mouse genome. This centered on INSERTs with including their genomic locations flanking sequences, expression levels of hit genes, patterns trapped genes if trapping vector was used. It also archives...
Regeneration of peripheral differentiated tissue in mammals is rare, and regulators this process are largely unknown. We carried out a forward genetic screen mice using N-ethyl-N-nitrosourea mutagenesis to identify mutations that affect regenerative healing vivo. More than 400 pedigrees were screened for closure through-and-through punch wound the mouse ear. This led identification single pedigree with heritable, fast, wound-healing phenotype. Within 5 wk after ear-punch, threefold decrease...
Nemaline myopathy (NM) is one of the most common forms congenital myopathy, and affects either fast myofibers, slow or both. However, an animal model for with fast-myofiber-specific atrophy not available. Furthermore, mutations in leiomodin-3 (LMOD3) gene have recently been identified a group individuals NM. it clear how loss LMOD3 leads to Here, we report mouse mutant which piggyBac (PB) transposon inserted into Lmod3 disrupts its expression. Lmod3(PB/PB) mice show severe muscle weakness...
We aimed to analyze the optimal timing of enteral nutrition (EN) in treatment sepsis and its effect on sepsis-associated acute kidney injury (SA-AKI.).The MIMIC-III database was employed identify patients with who had received EN. With AKI as primary outcome variable, receiver operating characteristic (ROC) curves were utilized calculate cut-off time early EN (EEN). Propensity score matching (PSM) control confounding effects. Logistic regressions propensity score-based inverse probability...
Leiomodin proteins, Lmod1, Lmod2 and Lmod3, are key regulators of the thin filament length in muscles. While Lmod1 is specifically expressed smooth muscles, both Lmod3 striated muscles including cardiac skeletal We others have previously shown that mainly function mutant mice display disorganized sarcomere. protein has been found to act as an actin nucleator cell-free assays cultured rat chicken cardiomyocytes. To better understand vivo, we identified characterized a piggyBac (PB)...
Significance Genome-wide, phenotype-driven mutagenesis in animal models could provide an unbiased way to decode a gene’s functions and its role diseases. Here we have generated piggyBac ( PB ) transposon-based first-generation (F1) dominant screening system mice, which provides unprecedented opportunities conduct highly efficient affordable genome-wide phenotypic screen for individual investigator single laboratory. Using this system, carried out F1 growth retardation discovered 5 isolated...
Blepharophimosis, ptosis, epicanthus inversus syndrome (BPES) is an autosomal dominant genetic disorder characterized by small palpebral fissures and other craniofacial malformations, often with (type I) but could also without II) premature ovarian failure. While mutations of the forkhead transcription factor FOXL2 are associated likely be responsible for many BPES cases, how affects development remain to understood. Through a large-scale piggyBac (PB) insertion mutagenesis, we have...
The Rab protein family is the largest of small GTP-binding proteins.Among them, RabG genes are known to be responsive abiotic stresses, but molecular mechanisms stress responses mediated by in plants poorly understood.To investigate mechanism AhRabG gene peanut, transgenic overexpressing (S6) with relatively higher salinity resistance than non-transgenic (S7) were obtained.Digital expression (DGE) sequencing was performed leaves S6 and S7 before after J.M. Sui et al. Genetics Molecular...
Concerted hormone secretion is essential for glucose homeostasis and growth. The oocyte testis gene 1 (Otg1) has limited information in mammals before. Human OTG1 been identified as an antigen associated with cutaneous T cell lymphoma, while worm Otg1 recently reported to be a vesicle trafficking regulator neurons. To understand the physiological role of its potential relation secretion, we characterized mutation caused by piggyBac transposon (PB) insertion mice. Oocyte encodes Golgi...
To evaluate the use of diagnosis-related groups (DRGs) tool to promote diagnosis/treatment ability and quality endocrinology department under new policy grouping payment-related disease diagnosis.We compared income structure in a 3a general hospital between first half 2019 same period 2021. We also observed changes cost efficiency indexes (CEIs), time (TEIs), case-mix index (CMI), number DRGs, risk weight (RW) proportion, surgery inpatient department. Furthermore, distribution inpatients...
This article has been retracted by the publisher.