A5020548800- Dental Implant Techniques and Outcomes
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Influenza Virus Research Studies
- Periodontal Regeneration and Treatments
- Hormonal and reproductive studies
- CRISPR and Genetic Engineering
- Orthodontics and Dentofacial Orthopedics
- Pancreatic function and diabetes
- Silk-based biomaterials and applications
- Renal and related cancers
- interferon and immune responses
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Dental Radiography and Imaging
- MicroRNA in disease regulation
- Selenium in Biological Systems
- Dental materials and restorations
- Ginseng Biological Effects and Applications
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Testicular diseases and treatments
- Quinazolinone synthesis and applications
- Skin Protection and Aging
Sichuan University
2012-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2021-2024
Wenzhou Medical University
2021-2024
State Key Laboratory of Oral Diseases
2019-2022
Northwest University
2020-2022
China XD Group (China)
2020
Guangdong Medical College
2015-2016
Northwest A&F University
2013-2016
Ministry of Agriculture, Livestock, and Food Supply
2015
Tianjin Medical University
2013
Abstract Objectives The hypoxia-inducible factor 1-α (HIF1α), a key molecule in mediating bone-vessel crosstalk, has been considered promising target for treating osteoporosis caused by gonadal hormones. However, senile osteoporosis, with accumulated senescent cells aged bone, distinct pathogenesis. study aimed at revealing the unknown role of HIF1α thus broadening its practical application osteoporosis. Materials and methods Femurs tibias were collected from untreated mice various ages (2...
As the most common sleep disorder, insomnia seriously affects people's everyday lives. Phytochemicals have been shown to excellent sleep-promoting effects. Therefore, this study was designed investigate whether Rg5 and Rk1 extracted from ginseng had effects explore their potential mechanisms. The results showed that could significantly lessen locomotor activity of mice promote quality index, including increasing amount in a pentobarbital sodium experiment with threshold dose. In parallel,...
Abstract Accompanying with diabetes mellitus‐induced osteoporosis (DM‐OS), diabetic patients show poor peri‐implant osteogenesis after implantation for dentition defect. Berberine (BBR), a candidate oral hypoglycemic agent, is promising agent treating DM‐OS. In this study, BBR was applied on DM rats and high‐glucose‐cultured bone mesenchymal stem cells (BMSCs) to investigate its therapeutic mechanism DM‐OS, thus laying theoretical basis the future application of in implant restoration....
Enhancing bone-vessel coupling to form high-quality vascular-rich peri-implant bone is crucial for improving implant prognosis in elder patients. Notably, hypoxia-inducible factor 1α (HIF1α) known promote osteogenesis-angiogenesis coupling; however, this effect remains be investigated aged owing the dual of HIF1α different organs. In study, inhibitor or activator was applied mice and their mesenchymal stem cells (BMSCs) investigate effects inner mechanism on osteogenesis angiogenesis...
Stem Leydig cells (SLCs) play a critical role in the development and maintenance of adult cell (ALC) population. SLCs also are present testis. Their identification, characteristics, regulation testis remain uncertain. Using single-cell RNA-seq, we found that mesenchymal stromal population may be involved ALC regeneration. Upon elimination, fraction begins to proliferate while different differentiate ALCs. Transcriptomic analysis identified five clusters can classified into two major groups...
Background Midazolam is a neurological drug with diverse functions, including sedation, hypnosis, decreased anxiety, anterograde amnesia, brain-mediated muscle relaxation, and anticonvulsant activity. Since it frequently used in children adolescents for extended periods of time, there risk that may affect their pubertal development. Here, we report potential effect the on development Leydig cells (LCs), testosterone (T)-producing testis. Methods Stem LCs (SLCs), isolated from adult rat...
Understanding the mechanisms that regulate pluripotency of embryonic stem cells (ESCs) is important to ensure their safe clinical use. CHIR99021 (CHIR)-induced activation Wnt/β-catenin signaling promotes self-renewal in mouse ESCs (mESCs). β-catenin functions individually or cooperates with transcription factors activate stemness such as c-Myc, Esrrb, Pou5f1, and Nanog. However relationship between core pluripotent factor, Kruppel-like factor 4 (also known GKLF EZF) signaling, remains...
Backgroud/Aims: Embryonic stem cells (ES cells) have the capacity to propagate indefinitely, maintain pluripotency, and differentiate into any cell type under defined conditions. As a result, they are considered be best model system for research early embryonic development. AICA ribonucleotide (AICAR) is an activator of AMP-activated protein kinase (AMPK) that thought affect ES function, but its role in fate decision unclear. Methods: In this study, we performed microarray analysis...
Spermatogenesis is an efficient, complex, and highly organized proliferation differentiation process that relies on multiple factors including testosterone produced by the Leydig cells. Although critical role played in spermatogenesis well recognized, mechanism which it works still not completely understood, partially due to inability specifically precisely monitor testosterone-dependent changes within developing germ Here we present single-cell RNA sequencing data from10,983 adult rat...
Background Testosterone plays a critical role in maintaining reproductive functions and well-beings of the males. Adult testicular Leydig cells (LCs) produce testosterone are generated from stem (SLCs) during puberty through adulthood. In addition, macrophages SLC regulatory niche for normal function. Age-related reduction serum contributes to number metabolic quality-of-life changes males, as well age-related immunological functions. How aging may affect function is still unclear. Methods...
Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, this self‐renewal capacity is governed by several crucial molecular pathways controlled specific regulatory genes epigenetic modifications. It reported that multiple regulators, such as miRNA factors, can be tightly integrated into cooperate maintain of ESCs. However, mouse ESCs serum‐containing medium seem heterogeneous due self‐activating differentiation signal MEK/ERK. Thus, seek...
Progenitor cells with ovulation-related tissue repair activity were identified defined markers (LGR5, EPCR, LY6A, and PDGFRA), but their potentials to form steroidogenic not known. This study shows that the can generate progenies different activities.
Clarithromycin (CAM) is a macrolide antibiotic that widely used in the treatment of respiratory tract infections, sexually transmitted diseases and infections caused by Helicobacter pylori Mycobacterium avium complex. Recent studies showed CAM was highly effective against multiple myeloma (MM) when combination with immunomodulatory drugs dexamethasone. However, related mechanism still unknown. As 3 agents are all respective regimen, we postulated might enhance effect drugs. We evaluated...
Abstract In full‐arch implant‐supported rehabilitation of patients with severe periodontitis, prediction lateral facial profile modified dental position remains a challenge, especially for protruded anterior teeth. This clinical report describes digital workflow to predict profiles and then guide the implant placement restoration fabrication.
This study investigated the effect of sodium selenite (Na2SeO3) on proliferation, cell cycle, apoptosis as well underlying mechanism in CNE‑2 nasopharyngeal carcinoma (NPC) cells. The line was treated with different concentrations Na2SeO3, and effects Na2SeO3 viability proliferation were evaluated using Cell Counting kit‑8 (CCK‑8) assay. Cellular cycle by flow cytomerty following Annexin V‑FITC/PI double staining PI single respectively; nuclei morphology stained DAPI Hoechst 333258 observed...
Human umbilical cord mesenchymal stem cells (hUCMSCs) are attractive therapeutic for tissue engineering to treat bone defects. However, how the can differentiate into remains unclear. Long non-coding RNAs (lncRNAs) that participate in many biological processes, including cell differentiation. In this study, we investigated profiles and functions of lncRNAs osteogenic differentiation hUCMSCs. We identified 343 differentially expressed during differentiation, which 115 were upregulated 228...
Abstract Secondary pneumonia due to Staphylococcus aureus ( S . ) causes significant morbidity and mortality. The aim of the research was designed a novel DNA vaccine encoding Mycobacterium tuberculosis secreted antigen Ag85A fused with influenza A virus (IAV) HA2 protein provide protection against both secondary infection vector efficiently expressed encoded in mammalian cells, as determined by RT-PCR, Western blotting immunofluorescence analysis. Mice were immunized intramuscular injection...
A novel DNA vaccine vector encoding the Mycobacterium tuberculosis secreted antigen Ag85A fused with influenza virus (IAV) HA2 protein epitopes, pEGFP/Ag85A-sHA2 (pAg85A-sHA2), was designed to provide protection against influenza. The encoded by efficiently expressed in mammalian cells, as determined reverse transcription polymerase chain reaction (RT-PCR) and fluorescence analyses. Mice were immunized intramuscular injection before challenge A/Puerto Rico/8/34 (PR8 virus). Sera splenocyte...
This study was purposed to evaluate the clinical significance of low molecular weight urinary proteins for diagnosis early renal damage in patients with multiple myeloma (MM). Medical records 278 MM Nanjing School Clinical Medicine from January 2004 May 2012 were analyzed retrospectively. These divided into 3 groups: glomerular group (n = 143), tubular 114) and normal 21). The laboratorial data compared among them. correlations retinol-binding protein (RBP) or...