A5036957212- Neonatal and Maternal Infections
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Oral microbiology and periodontitis research
- Chemical Synthesis and Reactions
- Clostridium difficile and Clostridium perfringens research
- Microbial Community Ecology and Physiology
- Aquaculture disease management and microbiota
- Peptidase Inhibition and Analysis
- Protist diversity and phylogeny
- Enzyme Production and Characterization
- Synthetic Organic Chemistry Methods
- Neonatal Health and Biochemistry
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Helicobacter pylori-related gastroenterology studies
- Chemistry and Chemical Engineering
- Synthesis of heterocyclic compounds
- Axial and Atropisomeric Chirality Synthesis
- Infective Endocarditis Diagnosis and Management
- Glycosylation and Glycoproteins Research
- Pediatric Hepatobiliary Diseases and Treatments
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
Université de Tours
2015-2024
Infectiologie Animale et Santé Publique
2013-2022
Institut Pasteur
2010-2014
Discovery Institute
2012
Sanford Burnham Prebys Medical Discovery Institute
2012
Université de Rouen Normandie
2005-2010
Normandie Université
2008
The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. By whole transcriptome analyses, we characterized glucose-dependent and CcpA-dependent gene regulation Clostridium difficile . About 18% of all C. genes are regulated by glucose, for which 50% depend on regulation. regulon comprises involved sugar uptake, fermentation amino acids metabolism, confirming...
ABSTRACT The Lmb protein of Streptococcus agalactiae is described as an adhesin that binds laminin, a component the human extracellular matrix. In this study, we revealed new role for in zinc uptake. We also identified two homologs, AdcA and AdcAII, redundant binding proteins combine with AdcCB translocon to form zinc-ABC transporter. Expression transporter controlled by concentration medium through zinc-dependent regulator AdcR. Triple deletion lmb , adcA adcAII or adcCB genes, impaired...
The nucleotide sequence of atlL, a gene encoding putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp encodes protein, AtlL, that appears as bifunctional autolysin with 29-amino acid signal peptide two enzymatic centres (N-acetylmuramoyl-l-alanine amidase N-acetylglucosaminidase) interconnected three imperfect repeated sequences displaying glycine-tryptophan motifs. In order to determine whether both lytic...
This work reports the characterization of first known peptidoglycan hydrolase (Acp) produced mainly during vegetative growth Clostridium perfringens. Acp has a modular structure with three domains: signal peptide domain, an N-terminal domain repeated sequences, and C-terminal catalytic domain. The purified recombinant displayed lytic activity on cell walls several Gram-positive bacterial species. Its hydrolytic specificity was established by analyzing Bacillus subtilis digestion products...
A gene encoding a putative peptidoglycan hydrolase was identified by sequence similarity searching in the Clostridium difficile 630 genome sequence, and corresponding protein, named Acd (autolysin of C. ) expressed Escherichia coli . The deduced amino acid shows modular structure with two main domains: an N-terminal domain exhibiting repeated sequences C-terminal catalytic domain. exhibits glucosaminidase domains Staphylococcus aureus Atl Bacillus subtilis LytD autolysins. Purified...
COV2HTML is an interactive web interface, which addressed to biologists, and allows performing both coverage visualization analysis of NGS alignments performed on prokaryotic organisms (bacteria phages). It combines two processes: a tool that converts the huge mapping or files into light specific containing information genetic elements; interface allowing real-time data with optional integration statistical results. To demonstrate scope COV2HTML, program was tested from published studies....
This study examined the role of two streptococcal histidine triad (Sht) proteins Streptococcus agalactiae in zinc homeostasis and complement resistance. We showed that Sht ShtII facilitate conjunction with metal-binding Lmb AdcAII. Here, we show Sht-family are functionally redundant overlapping roles uptake. Further, this work reveals although bind to factor H vitro did not influence survival human blood.
Abstract A rapid method has been developed for the synthesis of 3‐arylphthalides from readily available starting materials. We describe herein a direct approach based on simple Friedel‐Crafts condensation between an aromatic ester and aldehyde promoted by mixture phosphorus pentoxide methanesulfonic acid (Eaton's reagent) under metal solvent‐free conditions. Due to their similarity cytosporone E (a natural antibacterial phthalide), some synthesized compounds were tested as agents against...
Nearly all strains of Streptococcus agalactiae, the leading cause invasive infections in neonates, encode a type II-A clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system. Interestingly, S. agalactiae belonging to hypervirulent Sequence Type 17 (ST17) contain significantly fewer spacers their CRISPR locus than other lineages, which could be result less functional CRISPR-Cas Here, we revealed one large deletion ST17 cas promoter region and evaluated its impact on...
Summary The fru 2 metabolic operon of Streptococcus agalactiae encodes the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) enzyme II complex Fru (EIIB Fru2 , EIIA and EIIC ); R, a transcriptional activator with PTS regulatory domains (PRDs); d ‐allulose‐6‐phosphate 3‐epimerase; transaldolase; transketolase. We showed that transcription is induced during stationary phase growth in media incubation human cerebrospinal or amniotic fluids. ‐allose ‐ribose are environmental...
Streptococcus agalactiae is a leading cause of infections in neonates. This opportunistic pathogen colonizes the vagina, where it has to cope with acidic pH and hydrogen peroxide produced by lactobacilli. Thus, host, this bacterium possesses numerous adaptation mechanisms which pleiotropic regulators play major role. The transcriptional regulator CcpA (catabolite control protein A) previously been shown be involved carbon catabolite repression Gram-positive bacteria but also other functions....
Regulatory small RNAs (sRNAs) are involved in the adaptation of bacteria to their environment. CiaR-dependent sRNAs (csRNAs) controlled by regulatory two-component system (TCS) CiaRH, which is widely conserved streptococci. Except for Streptococcus pneumoniae and sanguinis, targets these csRNAs have not yet been investigated. agalactiae, leading cause neonatal infections, has four csRNA genes, namely, srn015, srn024, srn070, srn085. Here, we demonstrate importance direct repeat...