A5017000677- Epigenetics and DNA Methylation
- Genetic Associations and Epidemiology
- Genetics and Neurodevelopmental Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Birth, Development, and Health
- MicroRNA in disease regulation
- Alzheimer's disease research and treatments
- Genetic Syndromes and Imprinting
- Bioinformatics and Genomic Networks
- Health, Environment, Cognitive Aging
- Dementia and Cognitive Impairment Research
- Anesthesia and Neurotoxicity Research
- RNA modifications and cancer
- Cardiac, Anesthesia and Surgical Outcomes
- Intensive Care Unit Cognitive Disorders
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- melanin and skin pigmentation
- Cognitive Abilities and Testing
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Circular RNAs in diseases
- Biochemical Analysis and Sensing Techniques
- Metabolomics and Mass Spectrometry Studies
- Skin Protection and Aging
University of Southern Denmark
2016-2025
Odense University Hospital
2015-2024
Danish Pain Research Center
2021
University of Copenhagen
2004-2020
Rigshospitalet
2016-2020
Abstract Background Biological aging estimators derived from DNA methylation data are heritable and correlate with morbidity mortality. Consequently, identification of genetic environmental contributors to the variation in these measures populations has become a major goal field. Results Leveraging SNP more than 40,000 individuals, we identify 137 genome-wide significant loci, which 113 novel, association study (GWAS) meta-analyses four epigenetic clocks surrogate markers for granulocyte...
The genetic variant rs72824905-G (minor allele) in the PLCG2 gene was previously associated with a reduced Alzheimer's disease risk (AD). role of immune system signaling suggests it may also protect against other neurodegenerative diseases and possibly associates longevity. We studied effect on seven longevity, using 53,627 patients, 3,516 long-lived individuals 149,290 study-matched controls. replicated association AD we found an dementia Lewy bodies (DLB) frontotemporal (FTD). did not find...
Postoperative cognitive dysfunction is common, but it remains unclear whether there are long-term adverse effects of surgery combined with anesthesia. The authors examined the association between exposure to and level functioning in a sample 8,503 middle-aged elderly twins.Results from five tests were compared twins exposed surgery, classified as major, minor, hip knee replacement, or other, those reference group without using linear regression adjusted for sex age. Genetic shared...
Abstract The Danish Twin Registry (DTR) was established in the 1950s, when twins born from 1870 to 1910 were ascertained, and has since been extended include birth cohorts until 2009. DTR currently comprises of more than 175,000 140 cohorts. This makes oldest nationwide twin register among largest world. combination data several surveys, including biological samples repeated measurements on same individuals, national registers provides a unique resource for wide range studies. article an...
Mass spectrometry plays a central role in the characterisation of modified nucleotides, but pseudouridine is mass-silent post-transcriptional modification and hence not detectable by direct mass spectrometric analysis. We show use matrix-assisted laser desorption/ionisation (MALDI) that pseudouridines tRNA can be specifically cyanoethylated acrylonitrile without affecting uridines. The was then subjected to digestion with either RNase A or T1. Cyanoethylated fragments were identified...
Abstract Background Eye and hair colour is highly variable in the European population, largely genetically determined. Both linkage association studies have previously been used to identify candidate genes underlying this variation. Many of found were known as mutant mouse phenotypes or human genetic disease, but others, unsuspected pigmentation genes, also discovered. Results We assayed a population individuals Scottish origin using tristimulus colorimetry, order produce quantitative...
Purpose.: To investigate the diverse clinical manifestations, identify causative mutation and explain association with red hair in a family brittle cornea syndrome (BCS). Methods.: Eight members three generations underwent ophthalmic, dental, general medical examinations, including radiologic examination of spine. Bone mineral density (BMD) serum levels vitamin D, parathyroid hormone, biochemical markers for bone turnover were measured. Skin biopsies examined by light transmission electron...
Decline in cognitive abilities is a major concern aging individuals. A potential important factor for functioning of the central nervous system late-life stages KLOTHO ( KL ) gene. expressed various organs including brain and involved multiple biological processes, example, growth signaling. In present study, 19 tagging gene variants were studied relation to 2 measures function, 5-item composite score Mini Mental State Examination, 1,480 Danes 92–100 years age. We found that heterozygotes...
BackgroundMeasures based on DNA methylation, epigenetic clocks, have recently gained attraction as predictors of mortality and age-related pathologies. However, the origins variation in these measures are not well understood.MethodsIn a pooled sample 104 Swedish Danish twin pairs, we estimated, at mean age 70 (baseline) 79 years (follow-up), genetic environmental influences Horvath Levine clocks.FindingsA model incorporating additive (A) person-specific (E) best explained both clocks....
Abstract Environmental stresses such as extreme temperatures, dehydration and food deprivation may have distinct consequences for different age-classes males females across species. Here we investigate a natural population of the model organism Drosophila melanogaster . Males at ages 3, 19 35 days were tested stress resistance; i.e. ability flies to cope with starvation both cold hot temperatures. Further, measure metabolic efficiency, namely mitochondrial DNA copy number (mtDNA CN) in sexes...
Large numbers of autosomal sites are found differentially methylated in the aging genome. Due to analytical difficulties dealing with sex differences X-chromosome content and X-inactivation (XCI) females, this has not been explored for X chromosome.Using data from middle age elderly individuals (age 55+ years) two Danish cohorts monozygotic twins Scottish Lothian Birth Cohort 1921, we conducted an X-chromosome-wide analysis age-associated DNA methylation patterns consideration stably...
Abstract The Y chromosome, a sex chromosome that only exists in males, has been ignored traditional epigenetic association studies for multiple reasons. However, differences aging‐related phenotypes and mortality could suggest critical role of the chromosomes aging process. We obtained blood‐based DNA methylation data on 624 men from four cohorts performed chromosome‐wide analysis to detect Y‐linked CpGs differentially methylated over age cross‐validated significant cohorts. identified...
Abstract Mosaicism in blood varies with age, and cross-sectional studies indicate that for women, skewness of X-chromosomal mosaicism increases age. This pattern could, however, also be due to less X-inactivation more recent birth cohorts. Skewed X-chromosome inactivation was here measured longitudinally by the HUMARA assay 67 septuagenarian octogenarian women assessed at 2 time points, 10 years apart, centenarian 2–7 apart. compared 293 age-matched twins born 1917–1923 1931–1937, 212...
Longevity is a complex phenotype influenced by both environmental and genetic factors. The contribution estimated at about 25%. Despite extensive research efforts, only few longevity genes have been validated across populations. Long-lived individuals (LLI) reach extreme ages with relative low prevalence of chronic disability major age-related diseases (ARDs). We tested whether the protection from ARDs in LLI can partly be attributed to factors calculating polygenic risk scores (PRSs) for...