A5106466203- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Animal Genetics and Reproduction
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Evolution and Genetic Dynamics
- Diphtheria, Corynebacterium, and Tetanus
- Microbial Metabolism and Applications
- Rabies epidemiology and control
- Asthma and respiratory diseases
- Reproductive tract infections research
- Escherichia coli research studies
- Insect symbiosis and bacterial influences
- Yersinia bacterium, plague, ectoparasites research
- Bartonella species infections research
- Infective Endocarditis Diagnosis and Management
- Receptor Mechanisms and Signaling
- Inhalation and Respiratory Drug Delivery
- Viral gastroenteritis research and epidemiology
- Actinomycetales infections and treatment
- Tropical and Extratropical Cyclones Research
Université de Montpellier
2025
Centre Hospitalier Universitaire de Montpellier
2025
North Carolina State University
2024
University of North Carolina at Greensboro
2021-2023
Aix-Marseille Université
2016-2022
Méditerranée Infection Foundation
2017-2022
Institut de Recherche pour le Développement
2016-2022
Service de Santé des Armées
2019-2022
Assistance Publique Hôpitaux de Marseille
2017-2020
Université de Versailles Saint-Quentin-en-Yvelines
2020
Bacteria are nonsexual organisms but capable of exchanging DNA at diverse degrees through homologous recombination. Intriguingly, the rates recombination vary immensely across lineages where some species have been described as purely clonal and others "quasi-sexual." However, estimating has proven a difficult endeavor estimates often substantially studies. It is unclear whether these variations reflect natural populations or due to differences in methodologies. Consequently, impact on...
Background: Microbial culturomics represents an ongoing revolution in the characterization of environmental and human microbiome.Methods: By using three media containing high salt concentration (100, 150, 200 g/L), halophilic microbial culturome a commercial table was determined.Results: Eighteen species belonging to Terrabacteria group were isolated including eight moderate 10 halotolerant bacteria.Gracilibacillus massiliensis sp.nov., type strain Awa-1 T ( 0CSUR P14410DSM 29726), is...
In West Africa, as in many other estuaries, enormous volumes of marine water are entering the continent. Fresh discharge is very low, and it commonly strongly linked to rainfall level. Some these estuaries inverse estuaries. During Great Sahelian Drought (1968–1993), their hyperhaline feature was exacerbated. This paper aims describe evolution two main African those Saloum River Casamance River, since end drought. Water salinity measurements were carried out over three five years according...
Abstract Background Although originally thought to evolve clonally, studies have revealed that most bacteria exchange DNA. However, it remains unclear what extent gene flow shapes the evolution of bacterial genomes and maintains cohesion species. Results Here, we analyze patterns within between >2600 Our results show fewer than 10% species are truly clonal, indicating purely asexual rare in nature. We further demonstrate taxonomic criterion ~95% genome sequence identity routinely used...
Over recent years, genomic information has increasingly been used for prokaryotic species definition and classification. Genome sequence-based alternatives to the gold standard DNA–DNA hybridization (DDH) relatedness have developed, notably average nucleotide identity (ANI), which is one of most useful measurements delineation in era. However, strictly intracellar lifestyle, few measurable phenotypic properties low level genetic heterogeneity made current criteria bacterial inapplicable...
A new bacterium, strain AT3T, was isolated by microbial culturomics from a faecal sample Frenchman after bariatric surgery. The isolate exhibited 96.6% 16S ribosomal RNA gene nucleotide sequence similarity with Anaerotruncus colihominis WAL 14565T = CCUG 45055T CIP 107754T. Phenotypic and genomic characteristics showed that the represents novel species, for which name massiliensis sp. nov. is proposed. type AT3T CSUR P2007T DSM 100567T.
Coxiella burnetii is the etiological agent of Q fever, a worldwide zoonosis able to cause large outbreaks. The disease polymorphic. Symptomatic primary infection named acute fever and associated with hepatitis, pneumonia, auto-immune complications while persistent focalized infections, mainly endocarditis, vascular occur in minority patients but are potentially lethal. In order evaluate genomic features, genetic diversity, evolution, as well determinants antibiotic resistance, pathogenicity,...
Microbial culturomics is a new subfield of postgenomic medicine and omics biotechnology application that has broadened our awareness on bacterial diversity the human microbiome, including vaginal flora diversity. Using culturomics, obligate anaerobic Gram-stain-negative rod-shaped bacterium designated strain khD1T was isolated in vagina patient with vaginosis characterized using taxonogenomics. The most abundant cellular fatty acids were C15:0 anteiso (36%), C16:0 (19%), iso (10%). Based an...
Abstract Three previously unidentified Gram‐positive anaerobic coccoid bacteria, strains KhD‐2 T , KHD 4 and Kh‐D5 isolated from a vaginal swab, were characterized using the taxonogenomics concept. The phylogenic analysis, phenotypic characteristics, genotypic data presented in this report attest that these three bacteria are distinct known bacterial species with standing nomenclature represent new Peptoniphilus species. Strain is most closely related to sp. DNF 00840 harei (99.7% 98.2%...
As part of the culturomics project aiming at describing human microbiota, we report in this study description new bacterial genus Raoultibacter gen. nov. that includes two species, is, R. massiliensis sp. and timonensis The type strain Marseille-P2849T was isolated from fecal specimen a healthy 19-year-old Saudi Bedouin, while Marseille-P3277T feces an 11-year-old pygmy female living Congo. Strain exhibited 91.4% 16S rRNA sequence similarity with Gordonibacter urolithinfaciens, its...
Abstract Homologous recombination is a key evolutionary force that varies considerably across bacterial species. However, how the landscape of homologous genes and within individual genomes has only been studied in few Here, we used Approximate Bayesian Computation to estimate rate along 145 Our results show greatly shapes many aspects genome architecture evolution. The genomic presents several signatures: rates are highest near origin replication most species, patterns generally appear...
Bartonella mastomydis sp. nov. strain 008 is the type of B. nov., a new species within genus Bartonella. This was isolated from Mastomys erythroleucus rodents trapped in Sine-Saloum region Senegal. Here we describe features this organism, together with complete genome sequence and its annotation. The 2 044 960 bp long genomes 38.44% G + C content contains 1674 protein-coding 42 RNA genes, including three rRNA genes.
Abstract Strain Marseille‐P2341 T , a nonmotile, nonspore‐forming, Gram‐stain‐positive anaerobic coccus, was isolated in the vaginal specimen of patient with bacterial vaginosis using culturomics. Its growth occurred at temperatures ranging from 25 to 42°C, pH between 6.5 and 8.5, NaCl concentrations lower than 5%. The major fatty acids were C 18:1n9 (27.7%) 16:0 (24.4%). genome is 1,671,491 bp long 49.48 mol% G+C content. It composed 1,501 genes: 1,446 protein‐coding genes 55 RNA s. shared...
Strain AUS118T was isolated from an Argas lagenoplastis tick collected the nest of a Petrochelidon ariel (fairy martin) in Australia 2013. Microscopic observation infected cell cultures indicated this strain had morphology and intracellular location typical Rickettsia species. Phylogenetic analysis based firstly on multi-locus sequence subsequently whole genome demonstrated that most closely related to, but divergent japonica heilongjiangensis . We therefore propose creation novel species,...
A strictly anaerobic, Gram-stain-positive, non motile and non-spore-forming rod-shaped bacterium, strain Marseille-P2666T, was isolated using the culturomics approach from a vaginal sample of French patient suffering bacterial vaginosis. Cells were saccharolytic negative for catalase, oxidase, urease, nitrate reduction, indole production, hydrolysis aesculin gelatin. Strain Marseille-P2666T exhibited 97.04 % 16S rRNA sequence similarity to Collinsella tanakaei type YIT 12063T,...
Butyricimonas phoceensis strain AT9 (= CSUR 2478 = DSM 100838) was isolated from a stool sample morbidly obese French patient living in Marseille using the culturomics approach. The genome of this Gram-negative-staining, anaerobic and non–spore forming rod bacillus is 4 736 949 bp long contains 3947 protein-coding genes. Genomic analysis identified 173 genes as ORFans (4.5%) 1650 orthologous proteins (42%) not shared with closest phylogenetic species, virosa. Its major fatty acid branched...