A5072191200- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Receptor Mechanisms and Signaling
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- RNA regulation and disease
- Machine Learning in Bioinformatics
- Genetics and Neurodevelopmental Disorders
- Neuropeptides and Animal Physiology
- Nuclear Receptors and Signaling
- Neuroscience and Neuropharmacology Research
- Genetic Syndromes and Imprinting
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Invertebrate Immune Response Mechanisms
- Protein Degradation and Inhibitors
- Insect Resistance and Genetics
- Inflammasome and immune disorders
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Sichuan University
2018-2025
University of Münster
2001-2021
Medizinische Hochschule Brandenburg Theodor Fontane
2019
Universiti Sains Malaysia
2010-2013
Icahn School of Medicine at Mount Sinai
1991
Columbia University
1987
In nerve cells, a specialized protein synthetic machinery is thought to operate in local compartments of dendrites, particular beneath synaptic junctions, and thereby facilitate swift adjustments the postsynaptic repertoire situ. This notion has been supported by identification polyribosomes selected mRNAs those compartments. this study, we report discovery specific RNA polymerase III transcript dendrites. RNA, noncoding, 152-nucleotide-long, single-gene known as BC1 expressed almost...
Knock-in genome targeting risks: Comprehensive locus analysis is essential for precision chromosome-editing identification.
BC1 is a small developmentally regulated RNA that prevalent in nervous tissue. In order to determine if represents the transcriptional by-product of various repetitive brain identifier (ID) elements or independent transcript single few genes, we compared sequences population cDNA clones derived from vitro C-tailed RNA. Each 10 randomly selected revealed 5' domain was identical sequence ID element, followed by an internal region poly(A). 8 clones, found identical, nonrepetitive located at 3'...
Nonprotein-coding RNAs (npcRNAs) represent an important class of regulatory molecules that act in many cellular pathways. Here, we describe the experimental identification and validation small npcRNA transcriptome human malaria parasite Plasmodium falciparum. We identified 630 novel candidates. Based on sequence structural motifs, 43 them belong to C/D H/ACA-box subclasses nucleolar (snoRNAs) Cajal body-specific (scaRNAs). further observed exonization a functional H/ACA snoRNA gene, which...
Alzheimer’s disease (AD) is an age-related detrimental dementia. Amyloid beta peptides (Aβ) play a crucial role in the pathology of AD. In familial AD, Aβ are generated from full-length amyloid precursor protein (APP) via dysregulated proteolytic processing; however, case sporadic mechanism biogenesis remains elusive. circRNAs class transcripts preferentially expressed brain. We identified circRNA harboring Aβ-coding region APP gene termed circAβ-a. This circular RNA was detected brains AD...
Circular RNAs (circRNAs) are an emerging class of RNA molecules that have been linked to human diseases and important regulatory pathways. Their functional roles still under investigation, often hampered by inefficient circRNA formation in ex vivo. We generated intron-mediated enhancement (IME) system that-in comparison previously published methods-increases up 5-fold. This strategy also revealed undetected translation circRNA, e.g., circRtn4. Substantiated Western blots mass spectrometry we...
We experimentally identified and characterized 97 novel, non-protein-coding RNA candidates (npcRNAs) from the human pathogen Salmonella enterica serovar Typhi (hereafter referred to as S. typhi). Three were specific typhi, 22 restricted species 33 differentially expressed during typhi growth. also Pathogenicity Island-derived npcRNAs that might be involved in regulatory mechanisms of virulence, antibiotic resistance pathogenic specificity typhi. An in-depth characterization StyR-3 npcRNA...
New deep RNA sequencing methodologies in transcriptome analyses identified a wealth of novel nonprotein-coding RNAs (npcRNAs). Recently, was used to delineate the small npcRNA human pathogen Vibrio cholerae and 627 candidates were identified. Here, we report detection 223 V. by different cDNA library construction conventional methods. Remarkably, only 39 common both surveys. We therefore examined possible biasing influences analyses. Key steps, including tailing adapter ligations for...
Abstract Serotonin 5-HT2C receptor is a G-protein coupled excitatory that regulates several biochemical pathways and has been implicated in obesity, mental state, sleep cycles, autism, neuropsychiatric disorders neurodegenerative diseases. The activity of 5-HT2CR regulated via alternative splicing A to I editing exon Vb its pre-mRNA. Snord115 small nucleolar RNA expressed mouse neurons displays an 18-nucleotide base complementary For almost two decades this putative guide element wandered...
Mechanisms that trigger Aβ production in sporadic Alzheimer's disease are still obscure. We recently reported the expression of a human circular RNA (circAβ-a) encoded peptide precursor variant (Aβ175). Presently, we demonstrated AAV9 virus-expressed circAβ-a gave rise to extensive extracellular plaque depositions and microglial activation mouse brain; this recapitulates critical pathogenic hallmarks within AD model. Specifically developed antibodies detected robust endogenous Aβ175 HEK293...
Efficient RNA production is essential for therapeutic applications. Currently, mRNA generated by in vitro transcription with polymerases at a constant temperature. The success of these transcriptions depends on various factors including sequence composition and size. Sometimes, especially long RNAs, achieving efficient transcript yields are challenging. We developed novel approach incorporating high-temperature denaturation step. Our protocol includes the following two steps thermal cycling...
G protein-coupled sphingosine-1-phosphate receptor 1 (S1PR1), a drug target for inflammatory bowel disease (IBD), enables immune cells to egress from lymph nodes, but the treatment increases risk of immunosuppression. The functional signaling pathway triggered by S1PR1 activation in endothelial and its therapeutic application remains unclear. Here, we showed that is highly expressed IBD patients positively correlated with markers. Gi-biased agonist-SAR247799 activated reversed pathology male...
AbstractPoly(A) binding protein (PABP) binds non-protein-coding BC1 RNA and BC200 RNA, which contain adenosine-rich domains. Two combinations of the four PABP recognition motifs (RRMs), RRMs 1+2 3+4, bind with very strong affinities to various transcripts long stretches adenosine residues, whereas 2+3 weakly. While preferentially that only adenosines, 3+4 exhibit relatively high towards sequences are interspersed other nucleotides. Binding studies oligoribonucleotide(A)65...
Presence/absence patterns of retroposon insertions at orthologous genomic loci constitute straightforward markers for phylogenetic or population genetic studies. In birds, the convenient identification and utility these has so far been mainly restricted to lineages leading model birds (i.e., chicken zebra finch). We present an easy-to-use, rapid, cost-effective method experimental isolation repeat 1 (CR1) from virtually any bird genome potentially nonavian genomes. The application our little...
Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by dansyl-Edman method. Owing to availability only small quantities purified L31, sequencing methods were scaled down such that entire primary structure could be determined with 700 microgram protein. The techniques employed are described in detail. protein consists a single chain 62 amino acids, calculated molecular weight 6967. Four half-cystine residues identified at positions 16, 18, 37, and 40....
Abstract The third international conference on the genomic impact of eukaryotic transposable elements (TEs) was held 24 to 28 February 2012 at Asilomar Conference Center, Pacific Grove, CA, USA. Sponsored in part by National Institutes Health grant 5 P41 LM006252, goal bring together researchers from around world who study and mechanisms TEs using multiple computational experimental approaches. meeting drew close 170 attendees included invited floor presentations biology their impact, as...
Sperm-oocyte membrane fusion is necessary for mammalian fertilization. The factors that determine the of sperm with oocytes are largely unknown. So far, spermatozoon factor IZUMO1 and counter-receptor JUNO on oocyte has been identified as a protein requiring fusion. Some proteins such FIMP, SPACA6 TEME95, have proved not to directly regulate fusion, but their knockout will affect process oocytes. Here, we novel gene C11orf94 encoding testicular-specific small transmembrane emerges in...