A5029454626- Veterinary Oncology Research
- Microbial Inactivation Methods
- Glioma Diagnosis and Treatment
- Microfluidic and Bio-sensing Technologies
- Veterinary Orthopedics and Neurology
- Virus-based gene therapy research
- Cancer Research and Treatments
- Toxin Mechanisms and Immunotoxins
- Hedgehog Signaling Pathway Studies
- Ultrasound and Hyperthermia Applications
- Neuroscience and Neural Engineering
- Head and Neck Surgical Oncology
- Epilepsy research and treatment
- Neuroblastoma Research and Treatments
- Epigenetics and DNA Methylation
- Advancements in Transdermal Drug Delivery
- Angiogenesis and VEGF in Cancer
- Glycogen Storage Diseases and Myoclonus
- Neurological diseases and metabolism
- Thyroid Disorders and Treatments
- Barrier Structure and Function Studies
- Bone Tumor Diagnosis and Treatments
- Neurological disorders and treatments
- Neonatal and fetal brain pathology
- Chemokine receptors and signaling
Virginia Tech
2016-2025
Virginia–Maryland College of Veterinary Medicine
2016-2025
Atrium Health Wake Forest Baptist
2017-2024
Wake Forest Baptist Comprehensive Cancer Center
2022-2024
Wake Forest University
2015-2023
Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences
2013-2023
John Wiley & Sons (United States)
2022
The University of Texas at Austin
2021
Auburn University
2016
National Cancer Institute
2016
Abstract Background Therapeutic irreversible electroporation (IRE) is an emerging technology for the non-thermal ablation of tumors. The technique involves delivering a series unipolar electric pulses to permanently destabilize plasma membrane cancer cells through increase in transmembrane potential, which leads development tissue lesion. Clinically, IRE requires administration paralytic agents prevent muscle contractions during treatment that are associated with delivery pulses. This study...
The updated VCOG-CTCAE v2 guidelines contain several important updates and additions since the last update (v1.1) was released in 2011 published within Veterinary Comparative Oncology 2016. As Cooperative Group (VCOG) is no longer an active entity, original authors contributors to v1.0 v1.1 were consulted for input, additional co-authors sought expansion refinement of adverse event (AE) categories. includes expanded neurology, cardiac immunologic AE sections, addition procedural-specific...
BackgroundDespite promising treatments for breast cancer, mortality rates remain high and metastatic disease are limited. High-frequency irreversible electroporation (H-FIRE) is a novel tumor ablation technique that utilizes high-frequency bipolar electric pulses to destabilize cancer cell membranes induce death. However, there currently paucity of data pertaining immune system activation following H-FIRE other based techniques.MethodsHere, we utilized the mouse 4T1 mammary model evaluate...
Abstract Background Irreversible electroporation (IRE) is a new minimally invasive technique to kill undesirable tissue in non-thermal manner. In order maximize the benefits from an IRE procedure, pulse parameters and electrode configuration must be optimized achieve complete coverage of targeted while preventing thermal damage due excessive Joule heating. Methods We developed numerical simulations typical protocols based on previously published computed tomographic (CT) guided vivo...
Non-thermal irreversible electroporation (N-TIRE) has shown promise as an ablative therapy for a variety of soft-tissue neoplasms. Here we describe the therapeutic planning aspects and first clinical application N-TIRE treatment inoperable, spontaneous malignant intracranial glioma in canine patient. The ablation was performed safely, effectively reduced tumor volume associated hypertension, provided sufficient improvement neurological function patient to safely undergo adjunctive...
Sporadic gliomas in companion dogs provide a window on the interaction between tumorigenic mechanisms and host environment. We compared molecular profiles of canine with those human pediatric adult to characterize evolutionarily conserved mammalian mutational processes gliomagenesis. Employing whole-genome, exome, transcriptome, methylation sequencing 83 gliomas, we found alterations shared such as receptor tyrosine kinases, TP53 cell-cycle pathways, IDH1 R132. Canine showed high similarity...
Astrocytomas and oligodendrogliomas represent one third of histologically confirmed canine brain tumors. Our purpose was to describe the magnetic resonance (MR) imaging features intracranial astrocytomas examine for MR that differentiate these tumor types. Thirty animals with astrocytoma (14) or oligodendroglioma (16) were studied. All 12 located in cerebrum thalamus, remainder cerebellum caudal brainstem. Most (27/30) tumors associated both gray white matter. The signal characteristics...
Nonthermal irreversible electroporation (NTIRE) is a novel, minimally invasive technique to treat cancer, which unique because of its nonthermal mechanism tumor ablation. This paper evaluates the safety an NTIRE procedure lesion normal canine brain tissue.The involved placing electrodes into targeted area in 3 dogs and delivering series short intense electric pulses. The voltages pulses applied were varied between dogs. Another dog was used as sham control. One additional treated at extreme...
Abstract Treatment of glioblastoma multiforme (GBM) is especially challenging due to a shortage methods preferentially target diffuse infiltrative cells and therapy-resistant glioma stem cell populations. Here we report physical treatment method based on electrical disruption cells, whose action depends strongly cellular morphology. Interestingly, numerical modeling suggests that while outer lipid bilayer induced by long pulses (~100 μs) enhanced for larger short (~1 result in high fields...
Irreversible electroporation (IRE) is a non-thermal focal ablation technique that uses series of brief but intense electric pulses delivered into targeted region tissue, killing the cells by irrecoverably disrupting cellular membrane integrity. This study investigates if there an improved local anti-tumor response in immunocompetent (IC) BALB/c versus immunodeficient (ID) nude mice, including potential for systemic protective effect against rechallenge. Subcutaneous murine renal carcinoma...
Irreversible electroporation (IRE) is a novel nonthermal ablation technique that has been used for the treatment of solid cancers. However, it not evaluated use in brain tumors. Here, authors report on safety and feasibility using NanoKnife IRE system spontaneous intracranial gliomas dogs.Client-owned dogs with telencephalic glioma shown MRI were eligible. Dog-specific plans generated by MRI-based tissue segmentation, volumetric meshing, finite element modeling. After biopsy confirmation...
High-frequency irreversible electroporation is a nonthermal method of tissue ablation that uses bursts 0.5- to 2.0-microsecond bipolar electric pulses permeabilize cell membranes and induce death. has potential advantages for use in neurosurgery, including the ability deliver without inducing muscle contraction, inherent selectivity against malignant cells, capability simultaneously opening blood–brain barrier surrounding regions ablation. Our objective was determine whether high-frequency...
Magnetic resonance imaging (MRI) is used pervasively in veterinary practice for the antemortem diagnosis of intracranial tumors. Here, we provide an illustrated summary published MRI features primary and secondary tumors dogs cats, following PRISMA scoping review guidelines. The PubMed Web Science databases were searched relevant records, input from stakeholders was solicited to select data extraction. Sixty-seven studies moderate low-level evidence quality describing pathologically...
To analyze survival time and identify prognostic factors associated with outcome following discharge in dogs primary brain tumors treated palliatively.Prospective case series.51 5 histopathologic types of tumors.Owners examined from 2004 to 2008 were invited participate if had CT or MRI evidence a mass that was histopathologically confirmed as neoplasm upon death, survived for ≥ 48 hours after hospital discharge, treatments limited administration prednisone phenobarbital. Prognostic factors,...
To report the frequency, types, and risk factors for acute perioperative adverse events (AEs) in dogs that had ventral slot decompression (VSD) cervical intervertebral disc disease (IVDD).Retrospective, case-control study.Dogs (n = 546) with IVDD treated by VSD; 54 cases experiencing AE 492 controls.Historical, clinical, diagnostic, operative, outcome data were collected. graded using a Spine Adverse Events Severity (SAVES) system. Associations between development of hypothesized analyzed...
Background Intramedullary neoplasms of the canine spinal cord are infrequently reported. Objective To describe distribution, clinicopathologic characteristics, radiographic findings, and clinical features intramedullary tumors. Methods Retrospective series histologically confirmed Contingency tables were generated for categorical variables (breed, sex, treatment, pain, chief complaint, localization, histology, imaging, site). Associations assessed by F isher's exact, W ilcoxon rank sum test,...
Purpose Irreversible electroporation (IRE) has been developed as a promising minimally invasive treatment to ablate spontaneous brain tumors with pulsed electric fields in canine patients. The purpose of the study is determine Peleg‐Fermi parameters needed incorporate pulse number and duration into therapeutic planning IRE. Methods Seven patients were treated IRE for malignant glioma MRI‐based planning. method consists building patient‐specific finite element models using them compute used...
Background Irreversible electroporation (IRE) has been previously investigated in preclinical trials as a treatment for intracranial malignancies. Here, we investigate next generation high-frequency irreversible (H-FIRE), both monotherapy and combinatorial therapy, the of malignant gliomas. Methods Hydrogel tissue scaffolds numerical modeling were used to inform in-vivo H-FIRE pulsing parameters our orthotopic tumor-bearing glioma model. Fischer rats separated into five cohorts including...
The blood-brain-barrier (BBB) presents a significant obstacle to the delivery of systemically administered chemotherapeutics for treatment brain cancer. Irreversible electroporation (IRE) is an emerging technology that uses pulsed electric fields non-thermal ablation tumors. We hypothesized there minimal field at which BBB disruption occurs surrounding IRE-induced zone and this transient response can be measured using gadolinium (Gd) uptake as surrogate marker disruption. study was performed...