The diverse color patterns of cichlid fishes play an important role in mate choice and speciation. Here we develop the Nile tilapia (Oreochromis niloticus) as a model system for studying developmental genetics patterns. We identified 4 types pigment cells: melanophores, xanthophores, iridophores erythrophores, characterized their first appearance wild-type fish. mutated 25 genes involved melanogenesis, pteridine metabolism, carotenoid absorption cleavage pathways. Among genes, 13 had...

Excess abdominal fat is a common phenomenon in broiler chickens. Gut microbiota could regulate lipid metabolism through their effects on short-chain fatty acids (SCFAs) production. This study was conducted to investigate the potential relationship between development and cecal microorganism populations. Abdominal cecum contents were collected at 3, 7, 14, 21, 28, 35, 42 d of age. The results showed that weight increased with percentage higher 7 21 age than 3 (P < 0.05), it again 28 0.05)....

We report stimuli-responsive core–satellite assemblies of binary gold nanoparticles, linked by i-motif DNA, for live cell plasmonic imaging pH changes in the endocytic pathway.

Premelanosome protein (pmel) is a key gene for melanogenesis. Mutations in this are responsible white plumage chicken, but its role pigmentation of fish remains to be demonstrated. In study, we found that most fishes have 2 pmel genes arising from the teleost-specific whole-genome duplication. Both pmela and pmelb were expressed at high levels eyes skin Nile tilapia. We mutated both tilapia using CRISPR/Cas9. Homozygous mutation resulted yellowish body color with weak vertical bars...

There has been controversy over whether the chromogenic substances of xanthophores and erythrophores are pteridines or carotenoids in fish. In this study, we first extracted quantified from tilapia, respectively. The results showed that were significantly higher than those xanthophores, both types cells. Then, established homozygous mutant lines key genes for pteridine synthesis (xdh) carotenoid metabolism (plin6) Nile tilapia. Compared with wild type (WT), there no significant changes body...

Nile tilapia (Oreochromis niloticus) is an important species in aquaculture and excellent model fish for studying teleost color patterns. Colony-stimulating factor 1 receptor a (csf1ra) critical xanthophores development body formation zebrafish guppy. In this study, we established csf1ra mutant lines with two different targets by CRISPR/Cas9 tilapia. Unlike the wild type (WT) vertical bars, homozygous mutants showed patterns, either uniformly gray (GT) or black tail (BT). Compared WT, had...

The main objective of this study was to explore the effects broiler embryonic injection Lactobacillus Plantarum on growth performance, lipid metabolism serum and liver, microbial diversity, short-chain fatty acids intestines after hatching. On d 14 incubation, 720 eggs Arbor Acres were randomly divided into 4 experimental groups: no treatment control (C), Treatments injected with stroke-physiological saline solution (S), Supernatant MRS medium culture lactobacillus (Q) spp. (J). Hatch rate...

Recently, molecular keypad locks have received increasing attention. As a new subgroup of smart biosensors, they show great potential for protecting information as security data processor, rather than merely recognition and quantitation. Herein, label-free electrochemically transduced Ag+ cysteine (Cys) sensors were developed. A lock model with reset function was successfully realized based on the balanced interaction metal ion its nucleic acid chemical ligands. The correct input "1-2-3"...

The effects of Dendrobium polysaccharides (PDC) on the functions human skin fibroblasts (HSFs) and expression matrix metalloproteinase-2 under high-glucose conditions exploration underlying mechanism remain unclear. We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis flow cytometry to evaluate cell viability apoptosis. collagen levels were determined by Sircol™ Collagen Assay. Real-time quantitative polymerase chain reaction (RT-PCR) was detect (MMP-2)...

Abstract Glioblastoma (GBM) is a highly malignant brain tumor, and conventional treatments such as surgical resection chemotherapy have limited effectiveness, with high rate of recurrence poor prognosis for patients. In 2011, the FDA approved novel therapy recurrent GBM known Tumor Treating Fields (TTFields). TTFields use alternating intermediate-frequency (200 kHz) low-intensity (1-3 V/cm) electric fields to interfere tumor cells, inducing apoptosis inhibiting growth. This study aimed...

Abstract Mpv17 (mitochondrial inner membrane protein MPV17) deficiency causes severe mitochondrial DNA depletion syndrome in mammals and loss of pigmentation iridophores a significant decrease melanophores zebrafish. The reasons for this are still unclear. In study, we established an mpv17 homozygous mutant line Nile tilapia. developing mutants transparent due to the aggregation pigment granules disappearance vertical bars on side fish. Transcriptome analysis using skin fish at 30 dpf (days...

Gastric cancer is the most prevalent solid tumor form. Even after standard treatment, recurrence and malignant progression are nearly unavoidable in some cases of stomach cancer. GLIS Family Zinc Finger 3 (GLIS3) has received scant attention gastric research. Therefore, we sought to examine prognostic significance GLIS3 its association with immune infiltration Using public data from The Cancer Genome Atlas (TCGA), investigated whether gene expression was linked prognosis patients (STAD)....

This study plans to investigate whether miR-214-3p could bind CPLX2 regulate temozolomide (TMZ) resistance in glioma.The differential expression of and was determined by qRT-PCR Western blotting TMZ-resistant glioma tissues. Then, cells (U87/TMZ U251/TMZ) were established transfected with mimic, inhibitor, pcDNA3.1-CPLX2 or plus mimic evaluate the impact on proliferation, apoptosis TMZ U87/TMZ U251/TMZ cells. The binding relationship between reported dual-luciferase reporter assay.Higher...

Abstract Premelanosome protein (pmel) is a key gene for melanogenesis in vertebrates. Mutations this are responsible white plumage chicken, but its role pigmentation of fish remains to be demonstrated. In study we found that most fishes have two pmel genes arising from the teleost-specific whole genome duplication. Both pmela and pmelb were expressed at high levels eyes skin Nile tilapia. We mutated both tilapia using CRISPR/Cas9 editing. Homozygous mutation resulted yellowish body color...