A5054557624- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Bone Tissue Engineering Materials
- Photodynamic Therapy Research Studies
- Nerve injury and regeneration
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Genetics, Aging, and Longevity in Model Organisms
- Skin Protection and Aging
- Pluripotent Stem Cells Research
- bioluminescence and chemiluminescence research
- Muscle Physiology and Disorders
- Animal Genetics and Reproduction
- Antifungal resistance and susceptibility
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Telomeres, Telomerase, and Senescence
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- 3D Printing in Biomedical Research
Austrian Cluster for Tissue Regeneration
2016-2022
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2013-2022
Ludwig Boltzmann Institute for Digital Health and Prevention
2022
Mitochondrial-derived reactive oxygen species have been deemed an important contributor in sepsis pathogenesis. We investigated whether two mitochondria-targeted antioxidants (mtAOX; SkQ1 and MitoTEMPO) improved long-term outcome, lessened inflammation, organ homeostasis polymicrobial murine sepsis. 3-month-old female CD-1 mice (n = 90) underwent cecal ligation puncture (CLP) received (5 nmol/kg), MitoTEMPO (50 or vehicle 5 times post-CLP. Separately, 52 SkQ1-treated CLP were sacrificed at...
Therapeutic compensation of deficient bone regeneration is a challenging task and topic on-going search for novel treatment strategies.One promising approach improvement involves non-viral gene delivery using the morphogenetic protein-2 (BMP-2) to provide transient, local sustained expression growth factor.However, since efficiency low, this study focused on BMP-2 system, aiming poor transfection efficiency.First, native sequence was modified by codon optimisation altered inserting highly...
Tissue regenerative gene therapy requires expression strategies that deliver therapeutic effective amounts of transgenes.As physiological patterns are more complex than high-level a singular gene, we aimed at constitutive or inducible co-expression 2 transgenes simultaneously.Co-expression human bone morphogenetic protein and 7 (BMP2/7) from constitutively expressing doxycycline plasmids was evaluated in vitro C2C12 cells with osteocalcin reporter assays standard for osteogenic...
To improve the outcome after autologous nerve grafting in clinic, it is important to understand limiting variables such as distinct phenotypes of motor and sensory Schwann cells. This study investigated properties phenotypically different autografts a 6 mm femoral defect model rat, where respective branches distally inguinal bifurcation served homotopic or heterotopic autografts. Axonal regeneration target reinnervation was analysed by gait analysis, electrophysiology wet muscle mass...
Aging is accompanied by loss of subcutaneous adipose tissue. This may be due to reduced differentiation capacity or deficiency in DNA damage repair (DDR) factors. Here we investigated the role SNEVhPrp19/hPso4, which was implicated DDR and senescence evasion, adipogenic human stromal cells (hASCs). We showed that SNEV induced during adipogenesis localized both nucleus cytoplasm. Knockdown perturbed led accumulation hASCs upon oxidative stress. In addition, demonstrated required for fat...
Chondrogenic differentiation of adipose-derived stem cells (ASC) is challenging but highly promising for cartilage repair. Large donor variability chondrogenic potential raises the risk transplantation with reduced efficacy and a low potential. Therefore, quick potency assays are required to control isolated before cell transplantation. Current in vitro methods analyze capacity time-consuming, thus, novel enhancer tissue-specific promoter combination was used detection ASC bioassay. Human...
Reconstruction of bone defects and compensation deficient repair mechanisms represent important goals within the field regenerative medicine require novel safe strategies for translation into clinic. A non-viral osteogenic gene therapeutic vector system ('hybrid vectors') was generated, combining an improved morphogenetic protein 2 (BMP2) cassette single pro-osteogenic microRNAs (miR-148b-3p, miR-20-5p, miR-590b-5p), driven by U6 promoter. The vectors were tested in vitro their...
Monitoring of cell differentiation is a crucial aspect cell-based therapeutic strategies depending on tissue maturation. In this study, we have developed noninvasive reporter system to trace murine skeletal muscle differentiation. Either secreted bioluminescent (Metridia luciferase) or fluorescent (green protein [GFP]) was placed under the control truncated creatine kinase (MCK) basal promoter enhanced by variable numbers upstream MCK E-boxes. The engineered pE3MCK vector, coding triple...
Background: To date, the significant osteoinductive potential of bone morphogenetic protein 2 (BMP-2) non-viral gene therapy cannot be fully exploited therapeutically. This is mainly due to weak delivery and brief expression peaks restricting therapeutic effect. Objective: Our objective was test application minicircle DNA, allowing prolonged potential. It offers notable advantages over conventional plasmid DNA. The lack bacterial sequences resulting reduction in size, enables safe usage...
The increase in antibiotic resistance represents a major global challenge for our health systems and calls alternative treatment options, such as antimicrobial light-based therapies. Blue light has shown promising results regarding the inactivation of variety microorganisms; however, most often, blue (aBL) therapy is performed using wavelengths close to UV range. Here we investigated whether was possible with wavelength 475 nm. Both Gram-positive -negative bacterial strains were treated...