A5020353082- Adipose Tissue and Metabolism
- Thyroid Cancer Diagnosis and Treatment
- Thyroid Disorders and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Retinal Development and Disorders
- Cardiovascular Disease and Adiposity
- Photoreceptor and optogenetics research
- Birth, Development, and Health
- Cancer, Hypoxia, and Metabolism
- FOXO transcription factor regulation
- Regulation of Appetite and Obesity
- Electromagnetic Fields and Biological Effects
- Muscle metabolism and nutrition
- Microfluidic and Capillary Electrophoresis Applications
- Metabolism and Genetic Disorders
- Neuroendocrine Tumor Research Advances
- Cancer-related Molecular Pathways
- Analytical Chemistry and Chromatography
- Glaucoma and retinal disorders
- Photosynthetic Processes and Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Receptor Mechanisms and Signaling
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Proteomics Techniques and Applications
- Circular RNAs in diseases
German Center for Diabetes Research
2023-2025
University Hospital Leipzig
2014-2025
Deutsches Diabetes-Zentrum e.V.
2024-2025
Heinrich Heine University Düsseldorf
2024-2025
Leipzig University
2010-2024
Klinik und Poliklinik für Neurologie
2024
IFB Adiposity Diseases
2012
Berliner Hochschule für Technik
2009-2010
Hochschule Aalen
2002-2010
Klinik und Poliklinik für Augenheilkunde Universitätsklinikum Regensburg
2001
Obesity is a major health problem associated with global metabolic dysfunction and increased inflammation. It thus critical to identify the mechanisms underlying crosstalk between immune cells adipose tissue that drive cardiovascular in obesity. Expression of kallikrein-related serine protease 7 (KLK7) linked inflammation insulin resistance high fat diet (HFD)-fed mice. Here, we engineered mice macrophage-specific KLK7 knockout (KLK7MKO) investigate how loss impacts cell function...
The tumour suppressor protein p53 has functions in controlling the G(1)/S and G(2)/M transitions. Central regulators for progression from G(2) to mitosis are B-type cyclins complexed with cdc2 kinase. In mammals two cyclin B proteins found, B1 B2. We show that upon treatment of HepG2 cells 5-fluorouracil or methotrexate, levels increase while concentrations B2 mRNA, measured by RT-PCR LightCycler system, reduced. DLD-1 colorectal adenocarcinoma (DLD-1-tet-off-p53) mRNA drop after expression...
BACKGROUND: The protein p73, the first identified homologue of tumor suppressor gene p53 (also known as TP53), has been shown to induce apoptosis (programmed cell death), but its function in development not established. This study was undertaken investigate expression p73 liver tissue patients with hepatocellular carcinoma (HCC) and determine whether this any impact on prognosis. METHODS: In situ hybridization immunohistochemistry for detection RNA transcripts protein, respectively, were...
Abstract Recent studies have demonstrated that 1,25-dihydroxyvitamin D3 (calcitriol), the most biologically active metabolite of vitamin D, is a potent inhibitor both lectin- and antigen-driven human T lymphocyte proliferation. To better characterize this effect, we performed cell cycle analysis untreated calcitriol-treated peripheral blood mononuclear cells after PHA stimulation. By using metachromatic dye acridine orange flow cytometry, found calcitriol blocks transition from early, low...
The present study aimed to determine the effect of thyroid hormone dysfunction on brown adipose tissue activity and white browning in mice. Twenty randomized female C57BL/6NTac mice per treatment group housed at room temperature were rendered hypothyroid or hyperthyroid. In-vivo small animal 18F-FDG PET/MRI was performed effects hypo- hyperthyroidism BAT mass activity. Ex-vivo14C-acetate loading assay assessment thermogenic gene protein expression permitted analysis oxidative capacities WAT...
Obesity is one of the diseases with severe health consequences and rapidly increasing worldwide prevalence. Understanding complex network food intake energy balance regulation an essential prerequisite for pharmacological intervention obesity. G protein-coupled receptors (GPCRs) are among main modulators metabolism balance. They, instance, regulate appetite satiety in certain hypothalamic neurons, as well glucose lipid hormone secretion from adipocytes. Mutations some GPCRs, such...
Rather than a traditional growth factor, fibroblast factor-21 (FGF21) is considered to be metabolic hormone. In the current study, we investigated serum FGF21 levels in self-contained population of Sorbs. Serum concentrations were quantified by ELISA and correlated with IGF1 as well metabolic, renal, hepatic, inflammatory, cardiovascular parameters 913 Sorbs from Germany. Moreover, human protein secretion was FGF21-stimulated HepG2 cells. Median 2.1-fold higher subjects type 2 diabetes...
Abstract The functional and metabolic characteristics of specific adipose tissue (AT) depots seem to be determined by intrinsic mechanisms. We performed a comprehensive transcriptome profiling human AT from distinct fat unravel their unique features potentially explaining molecular mechanisms underlying distribution contribution health disease. Post-mortem samples five body donors 15 anatomical locations were collected. Global mRNA expression was measured Illumina® Human HT-12 v4 Expression...
Somatostatin receptors (SSTR) are expressed in various endocrine tumours. The expression of SSTR at the tumour cell surface confers possibility for diagnostic imaging and therapy tumours using radiolabeled somatostatin analogues. majority currently available analogues show a higher binding affinity SSTR2 subtype. To date, precise pattern subtypes 1–5 thyroid epithelial remains to be determined. We investigated mRNA SSTR1-5 benign malignant [20 cold nodules (CTNs), 20 toxic (TTNs), papillary,...
Objective Circulating levels of the adipokine adipocyte fatty acid-binding protein (AFABP) are increased in obesity. However, influence circulating AFABP on insulin sensitivity vivo remains unclear. Methods C57BL/6NTac mice (10 weeks) were treated over 8 weeks i.p. with saline (control) or recombinant (0.5 mg/kg/d). A comprehensive characterization metabolic parameters, body composition, and energy expenditure was performed. Furthermore, effect pancreatic β-cell responsiveness, hepatic...
The forkhead box transcription factor FOXO3a has recently been identified as central mediator of the cellular response to oxidative stress inducing cell cycle arrest or apoptosis. aim our study was investigate regulation in thyroid and determine whether alterations activity occur carcinogenesis. In vitro, we demonstrate that is negatively regulated by PI3K/Akt cascade promoting increased phosphorylation cytoplasmatic accumulation with decreased target genes p27kip (CDKN1B) Bim (BCL2L11), but...
We have recently found an increased expression of amyloid precursor protein (APP) in cold thyroid nodules that are difficult to classify as a truly benign neoplasm or lesion with the potential for further dedifferentiation. Since differences APP activity been other human cancers, we asked whether carcinogenesis might be associated altered and function. regulation was studied vitro differentiated (FRTL-5) dedifferentiated follicular carcinomas (FTC-133) cells after specific inhibition...
Abstract Objective Maternal hormonal status can have long-term effects on offspring metabolic health and is likely regulated via epigenetic mechanisms. We elucidated the of maternal thyroid hormones regulation leptin ( Lep ) transcription in adipose tissue (AT) subsequently investigated role DNA methylation at a upstream enhancer (UE) adipocyte biology. Results Pregnant mice treated with triiodothyronine (T3) produced reduced body weight, total fat mass, gonadal white (gWAT) mass 6 months...
Enantioseparations in nonaqueous capillary electrochromatography (CEC) are reported this study for the first time, using wide-pore aminopropyl silica gel coated with helically chiral poly(diphenyl-2-pyridylmethyl methacrylate) (PDPM) as stationary phase (CSP). The anodic electroosmotic flow (EOF) a methanolic solution of ammonium acetate was used migration neutral analytes through packed bed capillaries. Four different techniques, high-performance liquid chromatography (HPLC) common-size...
Several studies have demonstrated anti-diabetic and anti-obesogenic properties of visceral adipose tissue-derived serine protease inhibitor (vaspin) so evoked its potential use for treatment obesity-related diseases. The aim the study was to unravel physiological regulators vaspin expression secretion with a particular focus on role in brown tissue (BAT) biology. We analyzed effects obesogenic diets cold exposure liver white (AT) plasma levels. Vaspin isolated adipocytes during adipogenesis...
Vaspin is an adipokine which improves glucose metabolism and insulin sensitivity in obesity. Kallikrein 7 (KLK7) the first known protease target inhibited by vaspin a potential for treatment of metabolic disorders. Here, we tested hypothesis that inhibition KLK7 adipose tissue may beneficially affect function. Therefore, have inactivated Klk7 gene using conditional gene-targeting strategies mice. Klk7-deficient mice (ATKlk7 −/−) exhibited less weight gain, predominant expansion subcutaneous...
Phosphatase and tensin homolog (PTEN) hamartoma tumour syndrome (PHTS) is a rare disorder caused by germline mutations in the suppressor gene PTEN, key negative regulator of phosphatidylinositol 3-kinase (PI3K)/AKT signalling. Children with PHTS often develop lipomas, for which only surgical resection available as treatment. We investigated effects selective PI3K-inhibitor alpelisib on Pten-deficient lipomas. After incubation or non-selective PI3K inhibitor wortmannin, we analysed histology,...
Cold thyroid nodules (CTNs) represent a frequent endocrine disorder accounting for up to 85% of in population living an iodine-deficient area. Benign CTNs need be distinguished from cancer, which is relatively rare. The molecular etiology benign unresolved. To obtain novel insights into their pathogenesis, protein expression profiling was performed series 27 solitary (10 follicular adenoma and 20 adenomatous nodules) surrounding normal tissues using two-dimensional gel electrophoresis...