Background Obesity is causally associated with atherosclerosis, and adipose tissue ( AT )–derived exosomes may be implicated in the metabolic complications of obesity. However, precise role ‐exosomes atherogenesis remains unclear. We herein aimed to assess effect on macrophage foam cell formation polarization subsequent atherosclerosis development. Methods Results Four types isolated from supernatants ex vivo subcutaneous visceral VAT ) explants that were derived wild‐type mice high‐fat diet...

Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test a common method to measure key parameters Here, we use human cell line HK-2 as an example present procedures quantify oxygen consumption rate using Seahorse XFe96 extracellular flux analyzer. For complete details on and execution this protocol, please refer Ma et al. (2020).

Non-small-cell lung cancer (NSCLC) is the most common type in cancer, a leading cause of cancer-related death worldwide. Our previous study unraveled pro-cancer function IL-33 fueling outgrowth and metastasis human NSCLC cells. Herein, we determined that interfere with activity was an effective strategy for limiting tumor growth using preclinical model xenografts. blockade efficiently inhibited xenografts immune-deficient mice. Mechanistically, suppressed capacity Meanwhile, abrogated...

BACKGROUND: Histone H3 lysine 4 methylation is one of the most abundant epigenetic modifications, which has been recently linked to vascular remodeling in pulmonary hypertension (PH). SET1/MLL methyltransferase complexes comprise main enzymes responsible for methylating 4, yet their roles and PH are not fully understood. We aim assess contribution ASH2L, a core family member, pathogenesis PH. METHODS: Human artery specimens primary cells, smooth muscle cell (SMC)-specific ASH2L-deficient...

The high metastasis rate of lung cancer contributes to its low 5-year survival rate. Bone is a common complication in advanced cancer, adversely affecting postoperative recovery. This study investigates the effects DHA on macrophage polarization and underlying mechanisms. In vitro, was found inhibit M2 while promoting M1 macrophages, thereby reducing invasion migration cells. vivo, inhibited growth bone by modulating M1/M2 both tissues metastatic sites. addition, through CCL2/CCR2 pathway,...

Exposure of HL-60 leukemia cells to either 12-0-tetradecanoylphorbol-13-acetate (TPA), dimethylsulfoxide (DMSO), exogenous gangliosides GM3, GM1, or bovine brain ganglioside mixture (BBG) resulted in a marked inhibition the growth cells.The order inhibitory potency was TPA > GM3 DMSO BBG GMI.In contrast, sulfatides were without effect on cellular replication.Treatment with induced differentiation along monocyte/macrophage lineage, while treatment maturation granulocytic pathway.These effects...

Carnitine-acylcarnitine translocase (CACT) deficiency is a rare autosomal recessive metabolic disorder of mitochondrial long-chain fatty acid oxidation. Newborn screening via tandem mass spectrometry (MS/MS) technology enables early diagnosis. However, previous analyses MS/MS data patients showed that some results were misdiagnosed because they did not show typical acylcarnitine profiles CACT deficiency. This study aimed to identify additional indices assist the diagnosis deficiency.To...

The transcriptomic studies targeting circular RNAs (circRNAs) in bronchoalveolar lavage fluid (BALF) exosomes of acute respiratory distress syndrome (ARDS) patients caused by severe pneumonia have rarely been reported. This study aimed to screen and validate abnormally expressed circRNAs from BALF with ARDS then evaluate the diagnostic values these for ARDS.BALF was collected four healthy subjects. CircRNA expression profile obtained microarray analysis discovery cohort. differentially were...

Mitochondrial DNA (mtDNA) encodes thirteen core components of OXPHOS complexes, and its steady expression is crucial for cellular energy homeostasis. However, the regulation mtDNA machinery, along with sensing mechanism to energetic stresses, not fully understood. Here, we identified SQSTM1/p62 as an important regulator which could effectively induce effects were mediated by p38-dependent upregulation mitochondrial ribosomal protein L12 (MRPL12) in renal tubular epithelial cells (TECs), a...

Risks associated with preterm birth are unevenly distributed across all gestations. At earlier gestational ages, complications such as necrotizing enterocolitis (NEC) and late-onset sepsis (LOS) conditions significantly more common a shift in the composition of gut microbiome. Conventional bacterial culture techniques demonstrate that colonization microbiota infants differs from healthy-term infants. The current study aimed to investigate impact infancy on dynamic changes fecal at different...

Recent genome-wide association studies (GWASs) have identified 15q25.1 as a lung cancer susceptibility locus. Here, we sought to explore the direct carcinogenic effects of genetic variants in this region on risk developing adenocarcinoma (ADC). Five common SNPs (rs8034191, rs16969968, rs1051730, rs938682, and rs8042374) spanning locus were assayed case-control study examining cohort 301 ADCs 318 healthy controls. Stratification analysis by gender, smoking status, tumor, node, metastasis...

TWIST1 is an important basic helix-loop-helix protein linked to multiple physiological and pathological processes. Although believed be involved in vascular pathogenesis, its effects on homeostasis of smooth muscle cells (SMCs) remain poorly understood. Here, we show that levels were significantly elevated during SMC phenotypic switching vivo vitro. overexpression promoted SMCs, while siRNA targeting prevented cell transition. Mechanistically, decreased the level microRNA-143/145, which...

Radiotherapy is a common therapy in head and neck tumors, which may cause side effect radiation bone injury (RBI). Furthermore, it has been investigated that microRNA (miRNA) expression levels were altered after radiotherapy. Exosomes play role formation as miRNA containers, while affects exosomes composition, secretion, function. So, our objective to explore changes during radiotherapy identify the differentially expressed miRNAs (DE-miRs) plasma process of osteogenesis related...

Abstract Background Patients with deletions involving the long arm of chromosome 1 are rare. The PBX1 gene is located on 1q23.3. encodes a transcription factor which promotes protein–protein interaction and plays crucial role in several developmental processes. haploinsufficiency had been reported to lead syndromic congenital anomalies kidney urinary tract (CAKUT) humans. Case presentation In this research, 24-year-old woman (gravida 1, para 0) underwent amniocentesis at 22 weeks’ gestation...

Background and aims Radiotherapy is one of the major remedies for treatment cancer, including nasopharyngeal carcinoma (NPC). Radioresistance occurs very often in target cells that a large drawback cancer treated with radiotherapy. Livin involves over-growth cells. This study to investigate role livin radioresistance formation NPC Methods cell lines were exposed small doses irradiation establish model radioresistance, which development was evaluated. Results The expression observed cells,...

Mitochondrial injury of tubular epithelial cells (TECs) is the key pathogenic event underlying various kidney diseases and a potential intervening target as well. Our previous study demonstrated that ING2 ubiquitously expressed at tubulointerstitial area within kidneys, while its role in regulating TEC mitochondrial respiration not fully elucidated. To clarify roles homeostasis TECs pathogenesis acute ischemic injury, Western blot, PCR, immunofluorescence, immunoprecipitation, oxygen...

Immunofluorescence is a technique to visualize the localization of specific molecule targets within cells using specificity antibodies. Here, we describe protocol detect two different protein components in cell simultaneously. Antibody concentrations be used vary from and should optimized for types. In this protocol, perform co-immunofluorescence mitochondrial ribosomal L7/L12 (MRPL12) nuclear factor erythroid 2-related 2 (Nrf2), potential transcription MRPL12, HK-2 cells, as an example....

Heart failure with preserved ejection fraction (HFpEF) characterized by heart diastolic dysfunction. Epicardial adipose tissue (EAT) has been speculated to play an important role in HFpEF pathophysiological processes. In present study, EAT volume was significantly increased patients and high risk for developing compared healthy controls, it correlated CMR parameters indicative of dysfunction, particularly GLS LA reservoir, conduit, pump strains. These findings provided compelling evidence...

Motivation: Heart failure with preserved ejection fraction (HFpEF) frequently presents atrial fibrillation (AF). But the mechanism of AF in HFpEF are incompletely understood. Epicardial adipose tissue (EAT) is linked left volume and function changes.Goal(s): This study aims to evaluate relationships between EAT volumes patients HFpEF. Approach: All subjects were imaged a 3.0T Philips scanner. CMR parameters quantified stack short axis views perpendicular long ventricle. Results: was an...