A5003792089- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Immune Response and Inflammation
- Immune cells in cancer
- Neonatal and fetal brain pathology
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neuropharmacology Research
- S100 Proteins and Annexins
- Neurological Disease Mechanisms and Treatments
- Stress Responses and Cortisol
- Adenosine and Purinergic Signaling
- Tryptophan and brain disorders
- NF-κB Signaling Pathways
- Traumatic Brain Injury and Neurovascular Disturbances
- Pain Mechanisms and Treatments
- Anesthesia and Pain Management
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- Spine and Intervertebral Disc Pathology
- Nuclear Receptors and Signaling
- RNA regulation and disease
- Neuroendocrine regulation and behavior
- RNA Interference and Gene Delivery
Universitat Autònoma de Barcelona
2013-2023
Instituto de Hematología e Inmunología
2022
Inmunal (Spain)
2022
Institute for Biological Research “Siniša Stanković”
2016
Universitat de Barcelona
2016
Karolinska Institutet
2013
Instituto de Biomedicina de Sevilla
2013
Universidad de Sevilla
2013
Celula (United States)
2010
United Nations Economic Commission for Latin America and the Caribbean
2010
This study was designed to demonstrate the localization of poly-N-acetyl lactosamine residues in postnatal and adult rat brain, visualized by their specific binding a lectin obtained from Lycopersicon esculentum (tomato). Lectin histochemistry carried out on cryostat, paraffin, vibratome sections examined light microscopy. Selected were processed for electron Our results showed that tomato found relation blood vessels glial cells both brain. Since lectin-positive did not show GFAP...
Abstract Entrance of mesodermal precursors into the developing CNS is most well‐accepted origin microglia. However, contribution proliferation and death recruited microglial to final cell population remains be elucidated. To investigate apoptosis during development, we combined proliferating nuclear antigen (PCNA) immunohistochemistry, in situ detection DNA fragmentation (TUNEL), caspase‐3 immunohistochemistry with tomato lectin histochemistry, a selective marker. The study was carried out...
Cytokines are important intercellular messengers involved in neuron-glia interactions and the microglial-astroglial crosstalk, modulating glial response to brain injury lesion outcome. In this study, excitotoxic lesions were induced by injection of N-methyl-D-aspartate postnatal day 9 rats, cytokines interleukin-1 beta (IL-1beta), interleukin-6 (IL-6), tumour necrosis factor alpha (TNFalpha) transforming growth 1 (TGF-beta1) analysed ELISA and/or immunohistochemistry. Moreover,...
During the prenatal development of hippocampus, microglial cell precursors progressively occur in all subfields accordance with known ontogenetic gradients region (Dalmau et al., J. Comp. Neurol. 1997a;377:70–84). The present study follows regional distribution these and their morphological differentiation rat hippocampus from birth to postnatal (P) day 18. results demonstrate that cellular subregional microglia follow specific developmental different parts Ammon's horn dentate gyrus....
Abstract In order to evaluate proinflammatory cytokine levels and their producing cell types in the control aged rat brain after acute excitotoxic damage, both adult male Wistar rats were injected with N‐methyl‐ D ‐aspartate striatum. At different survival times between 6 hr 7 days lesioning, interleukin‐1 beta (IL‐1β), interleukin‐6 (IL‐6), tumor necrosis factor alpha (TNF‐α) analyzed by enzyme‐linked immunosorbent assay double immunofluorescence of cryostat sections using cell‐specific...
Caspase-3 has classically been defined as the main executioner of programmed cell death. However, recent data supports participation this protease in non-apoptotic cellular events including proliferation, cycle regulation, and differentiation. In study, astroglial cleavage caspase-3 was analyzed following excitotoxic damage postnatal rats to determine if its presence is associated with apoptotic death, or cytoskeletal remodeling. A well-characterized vivo model excitotoxicity studied, where...
Experimental autoimmune encephalomyelitis (EAE), a well-established model of multiple sclerosis, is characterised by microglial activation and lymphocyte infiltration. Induction EAE in Lewis rats produces an acute monophasic disease single peak disability followed spontaneous complete recovery subsequent tolerance to further immunizations. In the current study we have performed detailed analysis dynamics different populations cytokine profile along induction, peak, post-recovery phases this...
Interleukin‐10 (IL‐10) is a cytokine that plays crucial role in regulating the inflammatory response and immune reactions. In central nervous system (CNS), IL‐10 mainly produced by astrocytes microglia it upregulated after various insults, such as experimental autoimmune encephalomyelitis, middle cerebral artery occlusion, excitotoxicity traumatic brain injury. To better understand effects of normal injured CNS, we generated transgenic mice (termed GFAP‐IL‐10Tg) expressed murine gene under...
Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system. Interleukin (IL)−6 pleiotropic cytokine with potential role in MS. Here we used transgenic mice astrocyte-targeted production IL-6 (GFAP-IL6Tg) to study effect cuprizone-induced demyelination paradigm, which an experimental model de- and re-myelination, both hallmarks Our results demonstrated that cuprizone-treated GFAP-IL6Tg showed significant reduction astroglial especially microglial...
Microglial research has been constrained by a rolling series of dichotomies such as "resting versus activated" or "M1 M2". This Manichean perspective good bad microglia is in sharp contrast with the wide repertoire microglial functions, exerted from development to aging and diseases, brought light recent years. New designations continuously arising attempt describe different states transcriptomics other readouts may thus easily lead misleading, although unintentional, coupling categories...
The distribution and appearance of microglia cell precursors in the prenatal hippocampus were examined embryonic day 14 (E14) to E21 rats by nucleoside diphosphatase histochemistry. For comparison, differentiation astroglial cells was analyzed from E17 vimentin glial fibrillary acidic protein immunohistochemistry. Based on morphologic features, diphosphatase-positive microglial classified as ameboid primitive ramified cells. Ameboid present hippocampal primordium E14. As developed, however,...
We developed a double staining technique for simultaneous demonstration of astrocytes and microglial cells in histological brain sections cell cultures. The procedure included histochemical stain specific an immunocytochemical astroglial cells, with postponement the final visualization products until both reactions had been performed. First, were specifically but invisibly labeled by reaction nucleoside diphosphatase (NDPase). Then performing first parts glial fibrillary acidic protein...
When the homeostasis of central nervous system (CNS) is altered, microglial cells become activated displaying a wide range phenotypes that depend on specific site, nature activator, and particularly microenvironment generated by lesion. Cytokines are important signals involved in modulation molecular hence play pivotal role orchestrating activation. Among them, interleukin-6 (IL-6) pleiotropic cytokine described pathological conditions as potent inducer modulator activation, but with...
Traumatic brain injury is the greatest cause of disability and death in young adults developed world. The outcome for a TBI patient determined by severity injury, not only from initial insult but, especially, as product secondary injury. It proposed that this directly linked to neuro-inflammation, with production pro-inflammatory mediators, activation resident glial cells infiltration peripheral immune cells. In context, anti-inflammatory treatments are one most promising therapies dampen...
The subgranular zone of the dentate gyrus is an adult neurogenic niche where new neurons are continuously generated. A dramatic hippocampal neurogenesis decline occurs with increasing age, contributing to cognitive deficits. process intimately regulated by microenvironment, inflammation being considered a strong negative factor for this process. Thus, we hypothesize that reduction in aged brain could be attributed age-related microenvironmental changes towards pro-inflammatory status. In...