A5101714461- Plant-Microbe Interactions and Immunity
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- Extracellular vesicles in disease
- Aquatic Ecosystems and Phytoplankton Dynamics
- Osteoarthritis Treatment and Mechanisms
- Phytase and its Applications
- Bone health and treatments
- Bone Tissue Engineering Materials
- Synthesis and Characterization of Heterocyclic Compounds
- Soil Carbon and Nitrogen Dynamics
- Nematode management and characterization studies
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
Nanyang Normal University
2018-2025
Chinese Academy of Sciences
2018-2023
Institute of Genetics and Developmental Biology
2018-2023
Southwest Hospital
2019-2022
Army Medical University
2019-2022
Southwest University
2019-2022
Burnaby Hospital
2020
University of Chinese Academy of Sciences
2020
Plants recognize pathogens and activate immune responses, which usually involve massive transcriptional reprogramming. The evolutionarily conserved kinase, Sucrose non-fermenting-related kinase 1 (SnRK1), functions as a metabolic regulator that is essential for plant growth stress responses. Here, we identify barley SnRK1 WRKY3 transcription factor by screening cDNA library. interacts with in yeast, confirmed pull-down luciferase complementation assays. Förster resonance energy transfer...
Summary Powdery mildew disease, elicited by the obligate fungal pathogen Blumeria graminis f.sp. tritici ( Bgt ), causes widespread yield losses in global wheat crop. However, molecular mechanisms governing defense to are still not well understood. Here we found that TuACO3 , encoding 1‐aminocyclopropane‐1‐carboxylic acid (ACC) oxidase functioning ethylene (ET) biosynthesis, was induced infection of einkorn Triticum urartu which accompanied increased ET content. Silencing decreased...
Angiogenesis is essential for successful bone defect repair. In normal tissue repair, the physiological inflammatory response main regulator of angiogenesis through activity macrophages and cytokines secreted by them. particular, M2 which secrete high levels PDGF-BB are typically considered to promote angiogenesis. A hexapeptide [WKYMVm, (Trp-Lys-Tyr-Met-Val-D-Met-NH2)] has been reported modulate activities. However, underlying mechanisms WKYMVm regulates remain unclear. this study, possible...
Viruses often usurp host machineries for their amplification, but it remains unclear if hosts may subvert virus proteins to regulate viral proliferation. Here, we show that the 17K protein, an important virulence factor conserved in barley yellow dwarf viruses (BYDVs) and related poleroviruses, is phosphorylated by GRIK1-SnRK1 kinases, with (P17K) capable of enhancing abundance virus-derived small interfering RNAs (vsiRNAs) thus antiviral RNAi. Furthermore, P17K interacts RNA-degrading...
The invasion of osteoclasts into the cartilage via blood vessels advances process endochondral ossification, and dysregulation dynamic intercellular interactions results in skeletal dysplasias. Although regulation by growth plate chondrocytes has been reported detail, effect on remains to be determined. In this study, ATDC5 cells bone marrow mesenchymal stem were differentiated treated with conditioned medium obtained from macrophages osteoclast precursors osteoclasts. Exosomes inhibited or...
Freshwater reservoirs serve as vital water sources for numerous residential areas. However, the excessive presence of nutrients, such nitrogen and phosphorus, stimulates rapid algal proliferation, leading to occurrence blooms. To prevent this phenomenon, it is imperative conduct regular ecological surveys aimed at assessing quality monitoring dynamic composition aquatic biological communities within reservoir’s ecosystem. In study, seasonal changes in parameters spatial temporal distribution...
Abstract The balance between bone formation and resorption is closely related to homeostasis. Osteoclasts, originating from the monocyte/macrophage lineage, are only cell type possessing ability. Osteoclast overactivity thought be major reason underlying osteoclast‐related osteolytic problems, such as Paget's disease, aseptic loosening of prostheses inflammatory osteolysis; therefore, disruption osteoclastogenesis considered a crucial treatment option for these issues. WKYMVm, synthetic...
Summary The LRK10‐like receptor kinases (LRK10L‐RLKs) are ubiquitously present in higher plants, but knowledge of their expression and function is still limited. Here, we report functional analysis TtdLRK10L‐1, a typical LRK10L‐RLK durum wheat ( Triticum turgidum L. ssp. ). introns TtdLRK10L‐1 contained multiple kinds predicted cis ‐elements. To investigate the potential effect these ‐elements on function, two types transgenic lines were prepared, which expressed GFP‐tagged protein...
Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance model plant species. However, the functions of MAPK signaling pathways crop are largely unknown. Here we report function HvMKK1-HvMPK4-HvWRKY1 module barley immune system. HvMPK4 is identified to a negative role response against Bgh, as virus-induced gene silencing (VIGS) results enhanced whilst stably overexpressing leads super-susceptibility Bgh infection. Furthermore, HvMKK1 found specifically...
Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options promoting vasculogenesis within artificial grafts except exogenous EPC transplantation, which suffers from the source EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous recruitment regeneration using a bioinspired EPC-induced graft. The graft was created immobilizing...
Many animal viral proteins, e.g., Vpr of HIV-1, disrupt host mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. However, it is unknown whether plant viruses may use this mechanism in their pathogenesis. Here, we report that 17K protein, encoded barley yellow dwarf and related poleroviruses, delays G
To study the effect of fertilization on soil nematode communities in a paddy-upland rotation system, an ongoing thirty-three years long-term fertilizer experiment is conducted which includes seven treatments; unfertilized treatment (control), nitrogen (N), phosphorus (P), potassium (K) treatments (N, NP, NPK) and organic manure (M) combined with chemical (MN, MNP, MNPK). The community structure crop yields were determined 2012 2013. Overall total abundance was increased by incremental...
Triticum urartu (AA, 2n = 2x 14), a wild grass endemic to the Fertile Crescent (FC), is progenitor of A subgenome in common wheat. It belongs primary gene pool for wheat improvement. Here, we evaluated yellow rust (caused by Puccinia striiformis f. sp. tritici, Pst) reactions 147 T. accessions collected from different parts FC. The varied susceptibility strong resistance. In general, there were more with stronger resistance race CYR33 than CYR 32. most cases main form defense was moderate...
Transient gene expression via biolistic particle delivery is a widely used technique for functional analysis in plants. In this protocol we describe modified single-cell transient assay through transformation with inflow gun of the model PDS-1000/He system (Bio-Rad). This was originally optimized analyzing cell death activity and disease resistance function barley MLA (mildew locus A) proteins against powdery mildew fungus, which can be further adopted other purposes types plant some...
Abstract Background Inflammatory bowel diseases (IBD) are chronic gastrointestinal disorders that affect more than 270,000 Canadians, including over 7,000 children. Inflammation arising from IBD severely damages intestinal epithelial cells (IECs), however their role in disease pathogenesis is not fully understood, part due to a lack of vitro systems recapitulate the epithelium’s complex cellular composition. Enteroids an model where primary IECs isolated and cultured as 3D ‘mini guts.’ They...
Abstract Angiogenesis is essential for successful bone defect repair. In normal tissue repair, the physiological inflammatory response main regulator of angiogenesis through activity macrophages and cytokines secreted by them. particular, M2 which secrete high levels PDGF-BB are typically considered to promote angiogenesis. A hexapeptide [WKYMVm, (Trp-Lys-Tyr-Met-Val-D-Met-NH2)] has been reported modulate activities. However, underlying mechanisms WKYMVm regulates remain unclear. this study,...
Abstract Angiogenesis is essential for successful bone defect repair. In normal tissue repair, the physiological inflammatory response main regulator of angiogenesis through activity macrophages and cytokines secreted by them. particular, M2 which secrete high levels PDGF-BB are typically considered to promote angiogenesis. A hexapeptide [WKYMVm, (Trp-Lys-Tyr-Met-Val-D-Met-NH2)] has been reported modulate activities. However, underlying mechanisms WKYMVm regulates remain unclear. this study,...