It has been established that reactive oxygen species (ROS) such as H2O2 or superoxide anion is involved in bone loss-related diseases by stimulating osteoclast differentiation and resorption receptor activator of NF-κB ligand (RANKL) a critical osteoclastogenic factor expressed on stromal/osteoblastic cells. However, the roles ROS RANKL expression signaling mechanisms through which regulates genes are not known. Here we report increased intracellular levels xanthine/xanthine...

Increasing evidence suggests that N6-methyladenosine(m6A) has a vital role in cancer progression. Therefore, we aimed to explore the prognostic relevance of m6A-related genes oral squamous cell carcinoma (OSCC). First, Expression profiles were downloaded from The Cancer Genome Atlas (TCGA) and extracted afterwards. Then, cluster analysis principal component (PCA) used analyze genes. And differentially-expressed was performed R software. Furthermore, risk model constructed, crucial m6A...

The widespread prevalence of

Abstract Reactive oxygen species (ROSs) are involved in osteoporosis by inhibiting osteoblastic differentiation and stimulating osteoclastgenesis. Little is known about the role how ROS controls proliferation of osteoblasts. Mammalian target rapamycin, mTOR, a central regulator cell growth proliferation. Here, we report for first time that 5–200 μM hydrogen peroxide (H 2 O ) dose‐ time‐dependently suppressed without affecting viability mouse osteoblast line, MC3T3‐E1, human osteoblast‐like...

Tumstatin (Tum) is a powerful angiostatin that inhibits proliferation and induces apoptosis of tumorous vascular endothelial cells. A nonpathogenic anaerobic bacterium, Bifidobacterium longum ( BL), selectively localizes to proliferates in the hypoxia location within solid tumor. The aims this study were develop novel delivery system for Tum using engineered investigate inhibitory effect on tumor mice. vector enabled expression under control pBBADs promoter BL was constructed transformed...

Plenty of studies showed that the immune system was associated with cancer initiation and progression. This study aimed to explore prognostic biomarkers from immune-related genes (IRGs) in oral squamous cell carcinoma (OSCC).RNA-seq data were downloaded The Cancer Genome Atlas (TCGA) IRGs transcription factors (TFs) extracted. Then, co-expression network between TFs constructed using "WGCNA" package R software. Furthermore, a gene expression signature according predict OSCC prognosis its...

Abstract Background Colonoscopy is a classic diagnostic method with possible complications including abdominal pain and diarrhoea. In this study, gut microbiota dynamics related metabolic products during after colonoscopy were explored to accelerate microbiome balance through probiotics. Methods The fecal short-chain fatty acids (SCFAs) analyzed in four healthy subjects before colonoscopy, along seven individuals supplemented Clostridium butyricum . We employed 16S rRNA sequencing GC–MS...

Fibroblast activation protein (FAP) acts as a tumor promoter via epithelial-mesenchymal transition (EMT) in human oral squamous cell carcinoma (OSCC). The present study was designed to investigate the FAP targeting proteins and explore precise mechanism by which promotes EMT OSCC.Proteins interacting with were found filtered immunoprecipitation-mass spectrometry (IP-MS). Both DPP9 mRNA examined 90 paired OSCC samples matched normal tissue. knockdown conducted determine its function vitro...

Nuclear factor- κB (NF- κB) plays a pivotal role in the regulation of immune and inflammatory responses. The real-time expression level NF- reflects development ulcerative colitis (UC). Polydatin has vast pharmacological activities, including inhibiting production mediators, inducing antioxidants, regulating function, etc. purpose this study was to investigate potential inhibitory effects polydatin on pathway activation mouse UC model. results showed that treatment downregulated p65 activity...

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) the etiology of which has not yet been fully clarified. Cytokine interleukin-10 (IL-10) plays a central role in downregulating cascade UC and likely candidate for therapeutic intervention. However, its intravenous administration costly inconvenient. Therefore, we established novel IL-10 delivery system by transforming hIL-10-containing plasmid into B. longum (BL-hIL-10) investigated effects on 5% dextran sulfate sodium...

Background: The relationships among intestinal dysbiosis, bile acid (BA) metabolism disorders, and ulcerative colitis pathogenesis are now recognized. However, how specific strains regulate BA to alleviate is still unclear. This study investigated the effects of Bacteroides dorei on development acute elucidated underlying mechanisms. Methods: safety BDX-01 was evaluated in vitro vivo . 2.5% dextran sulfate sodium (DSS) induced C57BL/6 mice, Caco-2, J774A.1 cells were used evaluate...

Abstract Oral squamous cell carcinoma (OSCC) is the most common oral cancer. The molecular mechanisms of this disease are not fully understood. Our previous studies confirmed that dysregulated function long non-coding RNA (lncRNA) AC007271.3 was associated with a poor prognosis and overexpression promoted proliferation, migration, invasion, inhibited apoptosis in vitro, tumor growth vivo. However, underlying dysregulation remained obscure. In study, our investigation showed functioned as...

Conjugated linoleic acid (CLA) can prevent fatty accumulation induced by a high-fructose diet and improve lipid metabolism disorders in patients.We aimed to investigate the effect of CLA on obesity its possible mechanism.Eight-month-old male BKS.Cg-Dock7m +/+ Leprdb /JNju (db/db) mice (n = 12) were fed mix composed equivalent c9, t11-CLA t10, c12-CLA for 1 month. The body weight, water food intake, triglyceride (TG) total cholesterol (TC) levels was investigated. PPARα, PPARγ CD36 expression...

CD80 is an important co-stimulatory molecule that participates in the immune response. Soluble can induce T cell activation and overcome PDL1-mediated suppression. In this study, we aimed to construct recombinant

Intestinal probiotics are a primary focus area of current medical research. Probiotics such as bifidobacteria and lactobacilli can positively impact obesity other metabolic diseases by directly or indirectly affecting lipid metabolism. However, the precise mechanisms these effects remain unclear. In our previous work, novel strain Lactobacillus reuteri HI120 was isolated identified. expresses high levels linoleic isomerase, resulting in production large amounts conjugated acid (CLA) when...

Background: Head and neck squamous carcinoma (HNSC) poses a major threat to human life. The role of papillomavirus (HPV) infection in the initiation progression HNSC is becoming more widely accepted. HPV-positive (HPV+) has shown unique responses cancer therapies, which may be due differences immune cell infiltration. It critical determine how HPV are regulated. Methods: Transcriptome data from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) database were analyzed. Then, CIBERSORT...

To analyze the effect of conjugated linoleic acid (CLA) on glucose and lipid metabolism in obese diabetic (db/db) mice.db/db mice were randomized for treatment with saline or CLA mixture administered intragastrically. The changes body weight, dietary intake, water oral tolerance, triglyceride total cholesterol recorded after treatments. HE staining oil red O used to assess liver pathologies fatty content. expression levels PPARα, PPARγ, CD36, CHREBP SREBP-1c detected using real-time PCR...

Summary Many anticarcinogenic drugs kill tumour cells by inducing apoptosis. We examined the effects of hydrogen peroxide (H 2 O ) on arsenic trioxide (As 3 )‐induced cell killing. Low concentrations H (200 μ mol/l) inhibited ability As to induce apoptosis in Burkitt's lymphoma line Raji. altered form death from pyknosis/necrosis and also lowered degree killing . was capable preventing caspase‐3 activation induced Raji cells. Incubation with a phosphoinositide‐3 kinase (PI‐3K) inhibitor,...

Abstract The pathogenic mechanisms of Helicobacter pylori infection remain to be defined, and potential interventional microbiota are just beginning identified. In this study, gene‐set enrichment analysis (GSEA) was used integrate three H. microarray data sets from the gene expression omnibus database identified ten hallmark 35 Kyoto encyclopedia genes genomes (KEGG) pathways that differed between healthy ‐infected individuals. Weighted co‐expression network (WGCNA) performed on two key...

The aim of the present study was to determine relationship between p53 gene codon‑72 polymorphisms and hypertrophic scar formation following caesarean section (CS). Blood samples from 260 female patients were collected one week a CS for detection using molecular beacon‑coupled quantitative polymerase chain reaction technique. Patients had follow‑ups 12‑18 months observe formation. From these observations, occurrence investigated. Among with CCC/CCC genotype, nine scars 46 showed normal...