A5044059436- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Spinal Cord Injury Research
- Calcium signaling and nucleotide metabolism
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Adenosine and Purinergic Signaling
- Multiple Sclerosis Research Studies
- Axon Guidance and Neuronal Signaling
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Genetic Neurodegenerative Diseases
- Congenital heart defects research
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Zebrafish Biomedical Research Applications
- Renal and related cancers
- Photoreceptor and optogenetics research
- Renin-Angiotensin System Studies
- Signaling Pathways in Disease
- Adipose Tissue and Metabolism
- Immune cells in cancer
Karolinska Institutet
2015-2024
The Nature Conservancy
2024
Laboratory of Molecular Genetics
2019
German Center for Neurodegenerative Diseases
2014
University of Tübingen
2011-2012
Hertie Institute for Clinical Brain Research
2011-2012
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2005-2006
Oligodendrocytes have been considered as a functionally homogeneous population in the central nervous system (CNS). We performed single-cell RNA sequencing on 5072 cells of oligodendrocyte lineage from 10 regions mouse juvenile and adult CNS. Thirteen distinct populations were identified, 12 which represent continuum Pdgfra(+) precursor (OPCs) to mature oligodendrocytes. Initial stages differentiation similar across CNS, whereas subsets oligodendrocytes enriched specific brain. Newly formed...
Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis the central nervous system (CNS). It is unclear whether there a correlation between these and different (OL) states at adult stages. Here, we present bulk single-cell transcriptomics resources providing insights on how transitions occur. We found that post-natal OPCs from brain spinal cord similar transcriptional signatures. Moreover, OPC progeny of E13.5 electrophysiological profiles to...
Abstract Mature oligodendrocytes (MOLs) show transcriptional heterogeneity, the functional consequences of which are unclear. MOL heterogeneity might correlate with local environment or their interactions different neuron types. Here, we that distinct populations have spatial preference in mammalian central nervous system (CNS). We found type 2 (MOL2) is enriched spinal cord when compared to brain, while types 5 and 6 (MOL5/6) increase contribution OL lineage age all analyzed regions. MOL2...
In the mouse embryonic forebrain, developmentally distinct oligodendrocyte progenitor cell populations and their progeny, oligodendrocytes, emerge from three regions in a spatiotemporal gradient ventral to dorsal. However, functional importance of this developmental heterogeneity is unknown. Using genetic strategy ablate dorsally derived lineage cells (OLCs), we show here that areas which OLCs normally reside adult central nervous system become populated myelinated by origin. These ectopic...
Stem cells have a high therapeutic potential for the treatment of spinal cord injury (SCI). We shown previously that endogenous stem cell is confined to ependymal in adult which could be targeted non-invasive SCI therapy. However, are an understudied population. Taking advantage transgenic lines, we characterize appearance and during development. show vitro contained within these by birth. Moreover, juvenile cultures generate more neurospheres oligodendrocytes than ones. Interestingly, vivo...
New neurons generated in the adult dentate gyrus are constantly integrated into hippocampal circuitry and activated during encoding recall of new memories. Despite identification extracellular signals that regulate survival integration adult-born such as neurotrophins neurotransmitters, nature intracellular modulators required to transduce those remains elusive. Here, we provide evidence expression transcriptional activity nuclear factor T cell c4 (NFATc4) progenitor cells. We show NFATc4...
Following spinal trauma, the limited physiological axonal sprouting that contributes to partial recovery of function is dependent upon intrinsic properties neurons as well inhibitory glial environment. The transcription factor p53 involved in DNA repair, cell cycle, survival, and outgrowth, suggesting key modifier responses influencing functional following injury. Indeed, a cord dorsal hemisection injury model, we observed significant impairment locomotor −/− versus wild-type mice. cords...
Development of the spinal cord requires dynamic and tightly controlled expression numerous transcription factors. Forkhead Box protein J1 (FoxJ1) is a factor involved in ciliogenesis specifically expressed ependymal cells (ECs) adult central nervous system. However, using FoxJ1 fate-mapping mouse lines, we observed that also transiently by progenitors other neural subtypes during development. Moreover, knock-in line, discovered essential for embryonic to follow normal developmental...
NAADP is a second messenger that releases Ca2+ from intracellular stores. Surprisingly, it has been recently shown extracellular application of capable inducing release. This particularly important since the only mammalian enzymes known to catalyze synthesis this are located extracellularly. In present manuscript, we have investigated whether cells possess transport system transporting highly charged molecule into cells. Indeed, in RBL-2H3 cells, rat basophilic cell line, and SK-N-BE...
After an acute central nervous system injury, axonal regeneration is limited as the result of a lack neuronal intrinsic competence and presence extrinsic inhibitory signals. The injury fragments myelin insulating layer, releasing molecules to signal through membrane–bound Nogo receptor (NgR) complex. In this paper, we show that transcriptional pathway can interfere with myelin-dependent signaling, thereby promoting neurite outgrowth. Specifically, retinoic acid (RA), acting RA β (RAR-β),...
Axons in the central nervous system (CNS) do not regenerate while those peripheral (PNS) to a limited extent after injury (Teng et al., 2006). It is recognized that transcriptional programs essential for neurite and axonal outgrowth are reactivated upon PNS (Makwana 2005). However tools available analyze neuronal gene regulation vivo often challenging. The dorsal root ganglia (DRG) offer an excellent model because both CNS innervated by bifurcated axon originating from same soma. represent...
Summary Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis the central nervous system (CNS). It is unclear whether there a correlation between these and different transcriptional (OL) states at adult stages. Here we present bulk single-cell transcriptomics resource providing insights on how transitions occur. We show that E13.5 populations are not OPCs, exhibiting instead hallmarks of neural progenitors. A subset progenitors, which refer...
Abstract Oligodendrocytes (OLs), the myelinating cells of central nervous system, are transcriptionally heterogeneous, 1 origin and functional consequences which unknown. Functional heterogeneity MOLs might correlate with local environment or their interactions different neuron types. 2 Here, we show that distinct MOL populations have spatial preference in mammalian system differential susceptibility to traumatic spinal cord injury. We also generation is independent OPC developmental origin....
Axons in the central nervous system (CNS) do not regenerate while those peripheral (PNS) to a limited extent after injury (Teng et al., 2006). It is recognized that transcriptional programs essential for neurite and axonal outgrowth are reactivated upon PNS (Makwana 2005). However tools available analyze neuronal gene regulation vivo often challenging. The dorsal root ganglia (DRG) offer an excellent model because both CNS innervated by bifurcated axon originating from same soma. represent...