A5055593652- Veterinary Orthopedics and Neurology
- Muscle Physiology and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Veterinary Equine Medical Research
- Veterinary Oncology Research
- Herpesvirus Infections and Treatments
- Molecular Biology Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Human-Animal Interaction Studies
- Virus-based gene therapy research
- Machine Learning in Bioinformatics
- Microbial infections and disease research
- Lung Cancer Diagnosis and Treatment
- Spine and Intervertebral Disc Pathology
- Rabies epidemiology and control
- Bacterial Infections and Vaccines
- Autoimmune Neurological Disorders and Treatments
- Congenital heart defects research
- Head and Neck Surgical Oncology
- Vagus Nerve Stimulation Research
- Dupuytren's Contracture and Treatments
- Medical Imaging Techniques and Applications
- Cervical and Thoracic Myelopathy
- Neuroscience and Neural Engineering
- Antifungal resistance and susceptibility
Royal Veterinary College
2016-2025
University of London
2016-2025
University of Cambridge
2013-2024
Wellcome/MRC Cambridge Stem Cell Institute
2014-2024
University Hospitals Bristol and Weston NHS Foundation Trust
2023
University Hospitals Bristol NHS Foundation Trust
2023
Medical Research Council
2016
Wellcome Trust
2014
Addenbrooke's Hospital
2014
Stem Cell Institute
2014
The efficiency of central nervous system remyelination declines with age. This is in part due to an age-associated decline the phagocytic removal myelin debris, which contains inhibitors oligodendrocyte progenitor cell differentiation. In this study, we show that expression genes involved retinoid X receptor pathway are decreased ageing both myelin-phagocytosing human monocytes and mouse macrophages using a combination vivo vitro approaches. Disruption function young macrophages, antagonist...
Oligodendrocyte progenitors (OPs) arise from distinct ventral and dorsal domains within the ventricular germinal zones of embryonic CNS. The functional significance, if any, these different populations is not known. Using dual-color reporter mice to distinguish ventrally dorsally derived OPs, we show that, in response focal demyelination young adult spinal cord or corpus callosum, OPs undergo enhanced proliferation, recruitment, differentiation as compared with their counterparts, making a...
Remyelination is the regenerative response to demyelination. Although oligodendrocyte progenitor established as major source of remyelinating cells, there no conclusive evidence on whether mature, differentiated oligodendrocytes can also contribute remyelination. Using two different inducible myelin-CreER mouse strains in which mature were prelabeled by expression membrane-bound Green fluorescent protein, we found that after focal spinal cord demyelination, surrounding surviving labeled did...
In the mouse embryonic forebrain, developmentally distinct oligodendrocyte progenitor cell populations and their progeny, oligodendrocytes, emerge from three regions in a spatiotemporal gradient ventral to dorsal. However, functional importance of this developmental heterogeneity is unknown. Using genetic strategy ablate dorsally derived lineage cells (OLCs), we show here that areas which OLCs normally reside adult central nervous system become populated myelinated by origin. These ectopic...
Significance Bioelectronic modulation of the autonomic nervous system innervating spleen represents a new therapeutic avenue. Studies in rodents suffer from limitation that stimulation parameters and anatomy are not directly applicable to humans. This work demonstrates translation biological mechanisms large animal model with similar anatomical, histological, functional characteristics for derivation human-relevant parameters. Here, we show scientific process translating bioelectronic...
Feline infectious peritonitis (FIP) is the most common central nervous system (CNS) disease in cat and invariably fatal. Improved means of antemortem diagnosis required to facilitate clinical decision making. Information regarding magnetic resonance imaging (MRI) findings neurologic FIP currently limited, resulting need for better descriptions optimize its use as a diagnostic tool.To describe clinicopathologic features MRI cases confirmed FIP.Twenty-four client-owned cats with...
Bacterial meningitis (BM) and meningoencephalitis (BMEM) are associated with high case fatality rates neurologic sequelae in people, but limited data exists on outcome dogs.To report the clinicopathologic features, treatment of BM/BMEM dogs, a focus clinical presentation, relapse long-term neurological deficits.Twenty-four client-owned dogs diagnosed without empyema.Retrospective series from 5 veterinary referral hospitals between January 2010 August 2020.Twenty-four were included. Median...
Myokymia is a form of peripheral nerve hyperexcitability that can be focal or generalized. Information regarding myokymia in veterinary medicine currently limited, resulting need for better understanding this clinical sign. Describe the presentation, diagnostic findings, treatment, and outcomes dogs cats with facial (FM). Seven two clinically confirmed FM. Retrospective study. Clinical records from six referral institutions were reviewed to identify cases Signalment, test results, outcome...
ABSTRACT Background Myasthenia gravis (MG) is categorized into several subgroups, including seronegative MG. Seronegative human patients are well documented, but dogs remain clinically uncharacterized and their prevalence unknown. Objectives This study aims to evaluate the clinical presentation, diagnosis, treatment, outcome of canine MG subgroups. Animals One hundred sixty‐seven owner‐owned diagnosed with from three referral centers. Methods Retrospective case series. We classified...
Duchenne muscular dystrophy (DMD), a fatal musculoskeletal disease, is associated with neurodevelopmental disorders and cognitive impairment caused by brain dystrophin deficiency. Dog models of DMD represent key translational tools to study biology develop novel therapeutics. However, characterisation expression function in the canine lacking. We studied DE50-MD model that has missense mutation donor splice site exon 50. Using battery tests, we detected neurocognitive phenotype dogs,...
<ns3:p><ns3:bold>Background:</ns3:bold> The dystrophin gene has multiple isoforms: full-length (dp427) is principally known for its expression in skeletal and cardiac muscle, but also expressed the brain, several internal promoters give rise to shorter, N-terminally truncated isoforms with wider tissue patterns (dp260 retina, dp140 brain dp71 many tissues). These are believed play unique cellular roles both during embryogenesis adulthood, their shared sequence identity at mRNA protein levels...
<ns4:p><ns4:bold>Background:</ns4:bold> The dystrophin gene has multiple isoforms: full-length (dp427) is principally known for its expression in skeletal and cardiac muscle, but also expressed the brain, several internal promoters give rise to shorter, N-terminally truncated isoforms with wider tissue patterns (dp260 retina, dp140 brain dp71 many tissues). These are believed play unique cellular roles both during embryogenesis adulthood, their shared sequence identity at mRNA protein levels...
Intracranial EEG is the current gold standard technique for localizing seizures surgery, but it can be insensitive to tangential dipole or distant sources. Electrical Impedance Tomography (EIT) offers a novel method improve coverage and seizure onset localization. The feasibility of EIT has been previously assessed in computer simulation, which revealed an improved accuracy detection with compared intracranial EEG. In this study, slow impedance changes, evoked by cell swelling occurring over...
Abstract Background The information relating to the outcome specifically for juvenile dogs with meningoencephalitis of unknown etiology (MUE) is lacking. Objectives To describe clinical presentation, diagnostic findings, treatment, and in a cohort MUE <52 weeks old. Animals Thirty‐four client‐owned dogs. Methods Multicenter retrospective case series. Records from 5 referral centers were searched. Data was extracted medical records referring veterinarians contacted survival data if this...
Objectives To describe the MRI findings in a UK referral population of dogs with steroid‐responsive meningitis‐arteritis and to determine if they were associated any specific clinical features or outcomes. Materials Methods We performed multi‐centre retrospective case series diagnosed that underwent MRI. Blinded consensus review studies was described. The presence absence abnormalities analysed for significant associations presenting signs, results investigations Results Fifty‐three...
Pneumonectomy is the resection of all lung lobes from one side thorax. The clinical findings, treatment and outcome four cases feline chronic pyothorax managed with exploratory thoracotomy pneumonectomy are reported. All were initially medically thoracic drain placement antibiosis. However, resolution was not achieved medical therapy diagnostic imaging findings consistent an area abscessation or marked lobe consolidation identified, supporting a decision for surgical management. Surgical...
To date, few studies have investigated the clinical characteristics of thoracolumbar intervertebral disc protrusion (IVDP). The aim this retrospective study was to evaluate presentation and outcome dogs receiving medical or surgical treatment for IVDP. Eighty-four were included, with a median age 9.4 years. German shepherd Staffordshire bull terriers most common breeds. Significantly more surgically treated (n=53) had neurological deficits non-ambulatory, compared medically (n=31). Outcome...
Objectives To identify the prevalence of recurrence clinical signs after initial successful decompressive surgery for intervertebral disc extrusion in French bulldogs. Materials and Methods A medical record search was performed to bulldogs that experienced an outcome thoracolumbar or cervical extrusion. Collected information included signalment, neurological examination findings, location, presence vertebral malformations, kyphosis, type extent surgery. Decompressive not followed by...