A5027604191- Clinical Nutrition and Gastroenterology
- Organ Transplantation Techniques and Outcomes
- Cardiac Ischemia and Reperfusion
- Anesthesia and Neurotoxicity Research
- Barrier Structure and Function Studies
- Diet and metabolism studies
- Cardiac, Anesthesia and Surgical Outcomes
- Transplantation: Methods and Outcomes
- Metabolism and Genetic Disorders
- Intensive Care Unit Cognitive Disorders
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cancer-related molecular mechanisms research
- Gastroesophageal reflux and treatments
- Ethics and Legal Issues in Pediatric Healthcare
- Mitochondrial Function and Pathology
- Diabetes Treatment and Management
- Gastric Cancer Management and Outcomes
- Muscle metabolism and nutrition
- Renal Transplantation Outcomes and Treatments
- Family and Disability Support Research
- Amino Acid Enzymes and Metabolism
- MicroRNA in disease regulation
- Gut microbiota and health
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
Maastricht University Medical Centre
2012-2021
Maastricht University
2013-2021
University Medical Center
2017
Glucagon-like peptide 1 (GLP-1) is a hormone released from enteroendocrine L cells. Although first described as glucoregulatory incretin hormone, GLP-1 also suppresses inflammation and promotes mucosal integrity. Here, we demonstrate that plasma levels are rapidly increased by lipopolysaccharide (LPS) administration in mice via Toll-like receptor 4 (TLR4)-dependent mechanism. Experimental manipulation of gut barrier integrity after dextran sodium sulfate treatment, or ischemia/reperfusion...
<h3>Objective</h3> Colonic ischaemia is frequently observed in clinical practice. This study provides a novel insight into the pathophysiology of colon ischaemia/reperfusion (IR) using newly developed human and rat experimental model. <h3>Design</h3> In 10 patients small part that had to be removed for surgical reasons was isolated exposed 60 min (60I) with/without different periods reperfusion (30R 60R). Tissue not IR served as control. rats, 60I, 60I/30R, 60I/120R or 60I/240R (n=7 per...
To assess intestinal barrier function during human ischemia and reperfusion (IR).In a experimental model, 6 cm of jejunum was selectively exposed to 30 min (I) followed by 120 (R). A sham procedure also performed. Blood tissue sampled at all-time points. Functional assessed using dual-sugar absorption tests with lactulose (L) rhamnose Plasma concentrations citrulline, an amino acid described as marker for enterocyte were measured metabolic enterocytes restoration. Damage the epithelial...
Plasma levels of several amino acids are correlated with metabolic dysregulation in obesity and type 2 diabetes. To increase our understanding human amino-acid metabolism, we aimed to determine splanchnic interorgan handling. Twenty patients planned undergo a pylorus preserving pancreatico-duodenectomy were included this study. Blood was sampled from the portal vein, hepatic superior mesenteric inferior splenic renal radial artery during surgery. The difference between arterial venous...
Aim of this study was to draw comparisons between human colonic and jejunal ischemia-reperfusion sequelae in a vivo experimental model.In patients, generally has milder course than small intestinal ischemia-reperfusion. It is unclear which pathophysiologic processes are responsible for difference.In 10 patients undergoing surgery pancreaticoduodenectomy, 6 cm colon or jejunum isolated exposed 60 minutes ischemia followed by various reperfusion periods. Morphology (hematoxylin eosin),...
Sex differences in responses to intestinal ischemia-reperfusion (IR) have been recognized animal studies. We aimed investigate sexual dimorphism human small mucosal IR.In 16 patients (8 men and 8 women) undergoing pancreaticoduodenectomy, an isolated part of jejunum was subjected IR. In each patient, tissue blood collected directly after 45 minutes ischemia without reperfusion (45I-0R), 30 (45I-30R), 120 (45I-120R), as well a control sample not exposed IR, assess epithelial damage, unfolded...
Intestinal ischemia-reperfusion (IR) injury is a severe clinical condition, and unraveling its pathophysiology crucial to improve therapeutic strategies reduce the high morbidity mortality rates. Here, we studied dynamic proteome phosphoproteome in human intestine during ischemia reperfusion, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis gain quantitative information of thousands proteins phosphorylation sites, as well imaging (MSI) obtain spatial information. We...
Background. Ischemia-reperfusion injury is inevitable during intestinal transplantation (ITx) and executes a key role in the evolution towards rejection. Paneth cells (PCs) are crucial for epithelial immune defense highly vulnerable to ischemia-reperfusion injury. We investigated effect of ITx on PC after reperfusion (T0), follow-up, Moreover, we whether loss was associated with impaired graft homeostasis. Methods. Endoscopic biopsies, collected according center protocol at rejection...
Introduction: Intestinal transplantation (ITx) has become an accepted treatment for patients with irreversible intestinal failure, but remains a challenging procedure. Acute rejection is the most common complication. Ischemia reperfusion injury (IRI) plays pivotal role in cascade leading to rejection. We have shown that Paneth cells, important gatekeepers of crypts, are highly susceptible IRI humans. Therefore, our aim was study cell homeostasis and ITx patients. Methods: Archived endoscopic...
Introduction: Outcome after intestinal transplantation (ITx) has improved, however acute cellular rejection (ACR) remains a frequent challenge restricting long-term results. The gold standard for diagnosis of ACR is histological analysis graft biopsies. diagnostic criteria were defined during the 2003 VIIIth International Small Bowel Transplant Symposium. Although this report provided consensus international reporting, it not been validated in clinical setting. aim our study was to evaluate...
Background We developed a jejunal and colonic experimental human ischemia-reperfusion (IR) model to study pathophysiological intestinal IR mechanisms potential new ischemia biomarkers. Our objective was evaluate the safety of these models by comparing patients undergoing surgery with without in vivo IR. Methods A retrospective performed complication rates severity, based on Clavien-Dindo classification system, pancreatoduodenectomy (n = 10) 20 matched controls) or colorectal colon Secondary...