Zhongde Wang
A5103065061- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Pluripotent Stem Cells Research
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Renal and related cancers
- Long-Term Effects of COVID-19
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Sperm and Testicular Function
- Viral Infections and Immunology Research
- interferon and immune responses
- Virology and Viral Diseases
Utah State University
2016-2025
Tianjin Medical University
2023
The Fourth People's Hospital of Zibo City
2014-2016
Yangzhou University
2010-2015
University of Sioux Falls
2007-2015
City of Hope
2002-2010
Beckman Research Institute
2002-2010
Whitehead Institute for Biomedical Research
2004-2010
City Of Hope National Medical Center
2002-2007
University of Illinois System
2007
Cigarette smoke exposure is the major cause of chronic obstructive pulmonary disease (COPD). However, only a minority smokers develop significant COPD, and patients with asthma or asthma-like airway hyperresponsiveness eosinophilia experience accelerated loss lung function after cigarette exposure. Pulmonary inflammation characteristic feature lungs from COPD. Surprisingly, mediators this their contributions to pathogenesis varied natural history COPD are not well defined. Here we show that...
The recent emergence of B.1.1.529, the Omicron variant
Emergence of SARS-CoV-2 causing COVID-19 has resulted in hundreds thousands deaths. In search for key targets effective therapeutics, robust animal models mimicking humans are urgently needed. Here, we show that Syrian hamsters, contrast to mice, highly permissive and develop bronchopneumonia strong inflammatory responses the lungs with neutrophil infiltration edema, further confirmed as consolidations visualized by micro-CT alike clinical practice. Moreover, identify an exuberant innate...
Following emergence in late 2019, SARS-CoV-2 rapidly became pandemic and is presently responsible for millions of infections hundreds thousands deaths worldwide. There currently no approved vaccine to halt the spread only very few treatment options are available manage COVID-19 patients. For development preclinical countermeasures, reliable well-characterized small animal disease models will be paramount importance. Here we show that intranasal inoculation into Syrian hamsters consistently...
The expanding pandemic of coronavirus disease 2019 (COVID-19) requires the development safe, efficacious and fast-acting vaccines. Several vaccine platforms are being leveraged for a rapid emergency response1. Here we describe candidate (YF-S0) severe acute respiratory syndrome 2 (SARS-CoV-2) that uses live-attenuated yellow fever 17D (YF17D) as vector to express noncleavable prefusion form SARS-CoV-2 spike antigen. We assess safety, immunogenicity efficacy in several animal models. YF-S0...
Chronic inflammation containing CD8(+) lymphocytes, neutrophils, and macrophages, pulmonary emphysema coexist in lungs from patients with chronic obstructive disease. Although this inflammatory response is believed to cause the remodeling that seen these tissues, mechanism(s) by which causes have not been defined. Here we demonstrate interferon gamma (IFN-gamma), a prominent product of cells, alveolar enlargement, enhanced lung volumes, compliance, macrophage- neutrophil-rich when inducibly...
Reprogramming of a differentiated cell nucleus by somatic nuclear transplantation is an inefficient process. Following transfer, the donor often fails to express early embryonic genes and establish normal pattern chromatin modifications. These defects correlate with low number cloned embryos able produce stem cells or develop into adult animals. Here, we show that differentiation methylation state influence efficiency genomic reprogramming. First, neural cells, when used as donors for...
Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It involved in modest weight loss and improves glucose intolerance. However, the direct effects mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In current study, we demonstrated promoted Human Umbilical Vein Endothelial Cell (HUVEC) proliferation. was further this pro-proliferation effect mediated by irisin-induced activation extracellular signal–related kinase (ERK)...
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. The major cause of limited life span patients progressive lung disease. models have been generated 4 species (mice, rats, ferrets, and pigs) to enhance our understanding pathogenesis. Sheep may be particularly relevant animal model humans due similarities anatomy development two species. Here, we describe generation sheep for using CRISPR/Cas9 genome editing somatic cell...
ABSTRACT Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease endemic in parts of Asia. The etiologic agent, SFTS virus (SFTSV; family Bunyaviridae , genus Phlebovirus ) has caused significant morbidity and mortality China, South Korea, Japan, key features being intense fever, thrombocytopenia, leukopenia. Case fatality rates are estimated to be the 30% range, no antivirals or vaccines approved for use treatment prevention SFTS. There evidence that human...
Abstract Despite the development and deployment of antibody vaccine countermeasures, rapidly-spreading SARS-CoV-2 variants with mutations at key antigenic sites in spike protein jeopardize their efficacy. The recent emergence B.1.1.529, Omicron variant1,2, which has more than 30 protein, raised concerns for escape from protection by vaccines therapeutic antibodies. A test potential countermeasures against B.1.1.529 is activity pre-clinical rodent models respiratory tract disease. Here, using...
Abstract Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen causing febrile illness in humans, which can progress to manifestations, multi-organ failure, and death. Current mouse models of CCHFV infection reliably succumb challenge but vary their ability reflect signs disease similar humans. In this study, we established signal transducer activator transcription 2 ( STAT 2) knockout hamster model expand the repertoire animal pathogenesis that be used for therapeutic...
Animal models recapitulating human COVID-19 disease, especially severe are urgently needed to understand pathogenesis and evaluate candidate vaccines therapeutics. Here, we develop novel severe-disease animal for involving disruption of adaptive immunity in Syrian hamsters. Cyclophosphamide (CyP) immunosuppressed or
The prevalence of the Omicron subvariant BA.2.75 rapidly increased in India and Nepal during summer 2022, spread globally. However, virological features are largely unknown. Here, we evaluated replicative ability pathogenicity clinical isolates Syrian hamsters. Although found no substantial differences weight change among hamsters infected with BA.2, BA.5, or BA.2.75, lungs is higher than that BA.2 BA.5. Of note, causes focal viral pneumonia hamsters, characterized by patchy inflammation...
The golden Syrian hamster is the model of choice or only rodent for studying many human diseases. However, lack gene targeting tools in hamsters severely limits their use biomedical research. Here, we report first successful application CRISPR/Cas9 system to efficiently conduct hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three coding sequences different functional domains STAT2 protein, one KCNQ1, and PPP1R12C—and demonstrated that highly efficient introducing...
Introductory paragraph Since the emergence of SARS-CoV-2 causing COVID-19, world is being shaken to its core with numerous hospitalizations and hundreds thousands deaths. In search for key targets effective therapeutics, robust animal models mimicking COVID-19 in humans are urgently needed. Here, we show that productive infection lungs mice limited restricted by early type I interferon responses. contrast, Syrian hamsters highly permissive SARS- CoV-2 develop bronchopneumonia a strong...
Abstract During the Omicron wave, previous variants such as BA.2, BA.4, and BA.5 were replaced by newer with additional mutations in spike protein. These variants, BA.4.6, BQ.1.1, XBB, have spread different countries degrees of success. Here, we evaluated replicative ability pathogenicity BQ1.1, XBB clinical isolates male Syrian hamsters. Although found no substantial differences weight change among hamsters infected these subvariants, BQ.1.1 lung tissue was higher than that BA.4.6 BA.5. Of...
Abstract Recombinant modified vaccinia Ankara- and peptide-based IFN-γ ELISPOT assays were used to detect measure human CMV (HCMV)-specific CD8+ T cell responses the pp65 (UL83) immediate early protein 1 (IE1; UL123) gene products in 16 HCMV-infected infants children. Age at study ranged from birth 2 years. HCMV-specific cells detected 14 (88%) of children frequencies ranging 60 >2000 spots/million PBMC. Responses as day age with documented congenital infection. Nine responded both...