A5036594335- Bone Metabolism and Diseases
- Parathyroid Disorders and Treatments
- Bone health and treatments
- Bone health and osteoporosis research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Estrogen and related hormone effects
- Magnesium in Health and Disease
- Natural product bioactivities and synthesis
- Phytoestrogen effects and research
- Ion Transport and Channel Regulation
- Vitamin D Research Studies
- Metabolism, Diabetes, and Cancer
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Bone and Joint Diseases
- Growth Hormone and Insulin-like Growth Factors
- TGF-β signaling in diseases
- Inflammatory mediators and NSAID effects
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience and Neuropharmacology Research
- Bone Tissue Engineering Materials
- Phytochemistry and biological activity of medicinal plants
- Cytokine Signaling Pathways and Interactions
- Osteoarthritis Treatment and Mechanisms
Central Drug Research Institute
2016-2025
Council of Scientific and Industrial Research
2016-2025
Academy of Scientific and Innovative Research
2016-2025
University of Lucknow
2016-2021
Indian Institute of Technology Kanpur
2016
Endocrinology Research Center
2010-2012
Indian Institute of Toxicology Research
2011
Weatherford College
2010
Brigham and Women's Hospital
1999-2008
Harvard University
1999-2008
Th17 cells produce IL-17, and the latter promotes bone loss in collagen-induced arthritis mice. Blocking IL-17 action mouse model of rheumatoid reduces disease symptoms. These observations suggest that may be involved pathogenesis loss. However, role cell estrogen (E2) deficiency-induced is still not very clear. We investigated effect E2 on differentiation vivo mediated regulation osteoclast osteoblast differentiation. Additionally, functional block under was studied. In murine marrow cells,...
Aging is associated with changes in circulating levels of various molecules, some which remain undefined. We find that concentrations taurine decline aging mice, monkeys, and humans. A reversal this through supplementation increased the health span (the period healthy living) life mice monkeys. Mechanistically, reduced cellular senescence, protected against telomerase deficiency, suppressed mitochondrial dysfunction, decreased DNA damage, attenuated inflammaging. In humans, lower correlated...
We previously identified transcripts encoding a G protein-coupled, extracellular calcium/polyvalent cation-sensing receptor, RaKCaR, in rat kidney (D. Riccardi, J. Park, W.-S. Lee, G. Gamba, E. M. Brown, and S. C. Hebert. Proc. Natl. Acad. Sci. USA 92: 131–135, 1994), which was proposed to provide the mechanism for modulating variety of renal functions response changes Ca 2+ (E. Brown. In: Handbook Physiology. Bethesda, MD: Am. Physiol. Soc., 1992, sect. 8, vol. 2, chapt. 39, p. 1841–1916;...
The calcium-sensing receptor (CaR) is a G protein-coupled that plays key roles in extracellular calcium ion (Ca2+o) homeostasis parathyroid gland and kidney. Osteoblasts appear at sites of osteoclastic bone resorption during remodeling the "reversal" phase following preceding formation. Bone produces substantial local increases Ca2+o could provide signal for osteoblasts vicinity, leading us to determine whether such express CaR. In this study, we used mouse osteoblastic, clonal cell line...
The extracellular calcium ([Formula: see text])-sensing receptor (CaR) plays vital roles in [Formula: text] homeostasis, but no data are available on its expression small and large intestine. Polymerase chain reaction products amplified from reverse-transcribed duodenal RNA using CaR-specific primers showed >99% homology with the rat kidney CaR. Northern analysis a cRNA probe demonstrated 4.1- 7.5-kb transcripts all intestinal segments. Immunohistochemistry antisera clear basal staining...
Intracellular transduction pathways that are dependent on activation of the CaR by Ca(o)2+ have been studied extensively in parathyroid and other cell types, include cytosolic calcium, phospholipases C, A2, D, protein kinase C isoforms cAMP/protein A system. In this study, using bone marrow cells isolated from CaR-/- mice as well DN-CaR-transfected RAW 264.7 cells, we provide evidence expression plays an important role osteoclast differentiation. We also establish resultant stimulation PLC...
Metastasis of breast cancer to bone occurs with advanced disease and produces substantial morbidity. Secretion PTH-related peptide (PTHrP) from cells is thought play a key role in osteolytic metastases increased by transforming growth factor-beta (TGFbeta), which released resorbed bone. Elevated extracellular calcium (Ca2+(o)) also stimulates PTHrP secretion various normal malignant cells, an action that could potentially be mediated the Ca2+(o)-sensing receptor (CaR) originally cloned...
Abstract The cloning of a G protein–coupled, extracellular calcium (Ca2+e)-sensing receptor (CaR) from bovine parathyroid provided direct evidence that Ca2+e-sensing can occur through receptor-mediated activation proteins and their associated downstream regulators cellular function. CaR transcripts protein are present in various tissues humans other mammals involved Ca2+e homeostasis, including parathyroid, kidney, thyroidal C-cells. study was performed to determine whether bone marrow cells...
The G protein-coupled, extracellular calcium-sensing receptor (CaR) regulates parathyroid hormone secretion and cellular proliferation as well the functions of diverse other cell types. CaR resides in caveolae-plasma membrane microdomains containing receptors associated signaling molecules that are thought to serve "message centers." An additional mechanism for coordinating is presence scaffold proteins bind organize components signal transduction cascades. With use yeast two-hybrid system,...
Activated T cell has a key role in the interaction between bone and immune system. cells produce proinflammatory cytokines, including receptor activator of NF-κB ligand (RANKL), tumor necrosis factor α (TNF-α), interleukin 17 (IL-17), all which augment osteoclastogenesis. RANKL TNF-α are targeted by inhibitors such as denosumab, human monoclonal antibody, infliximab, neutralizes TNF-α. IL-17 is also an important mediator loss, antibody against undergoing phase II clinical trial for...
Discoveries in the last few years have emphasized existence of an enormous breadth communication between osteo-immune systems. These discoveries fuel novel approaches for treatment several bone pathologies including osteoporosis.
The parathyroid calcium-sensing receptor (CaR) plays a nonredundant role in systemic calcium homeostasis. In bone, Ca(2+)(o), major extracellular factor the bone microenvironment during remodeling, could potentially serve as an first messenger, acting via CaR, that stimulates proliferation of preosteoblasts and their differentiation to osteoblasts (OBs). Primary digests rat calvarial OBs express CaR assessed by RT-PCR, Northern, Western blot analysis, immunocolocalization with OB marker...
Abstract We recently reported that extracts made from the stem bark of Ulmus wallichiana promoted peak bone mass achievement in growing rats and preserved trabecular cortical strength ovariectomized (OVX) rats. Further, 6-C-β-D-glucopyranosyl-(2S,3S)-(+)-3',4',5,7-tetrahydroxyflavanol (GTDF), a novel flavonol-C-glucoside isolated extracts, had nonestrogenic bone-sparing effect on OVX Here we studied effects GTDF osteoblast function its mode action vivo osteogenic effect. stimulated...
Abstract Following a lead obtained from stem‐bark extract of Butea monosperma , two structurally related methoxyisoflavones; cajanin and isoformononetin were studied for their effects in osteoblasts. Cajanin had strong mitogenic as well differentiation‐promoting on osteoblasts that involved subsequent activation MEK‐Erk Akt pathways. On the other hand, exhibited potent anti‐apoptotic effect addition to promoting osteoblast differentiation parallel Unlike genistein or daidzein, none these...
Summary Isoflavones, a group of flavonoids, restricted almost exclusively to family Leguminosae are known exhibit anticancerous and anti‐osteoporotic activities in animal systems have been target for metabolic engineering commonly consumed food crops. Earlier efforts based on the expression legume isoflavone synthase ( IFS ) genes nonlegume plant species led limited success terms content transgenic tissue due limitation substrate enzyme. In this work overcome limitation, activation multiple...
Recently, we reported that extract of Dalbergia sissoo made from leaves and pods have antiresorptive bone-forming effects. The positive skeletal effect attributed because active molecules present in the sissoo. Caviunin 7-O-[β-D-apiofuranosyl-(1-6)-β-D-glucopyranoside] (CAFG), a novel isoflavonoid show higher percentage extract. Here, osteogenic potential CAFG as an alternative for anabolic therapy treatment osteoporosis by stimulating bone morphogenetic protein 2 (BMP2) Wnt/β-catenin...