A5090154847- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 detection and testing
- Full-Duplex Wireless Communications
- Reproductive tract infections research
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Viral-associated cancers and disorders
- COVID-19 Clinical Research Studies
- Energy Harvesting in Wireless Networks
- Urinary Tract Infections Management
- Cervical Cancer and HPV Research
- Viral gastroenteritis research and epidemiology
- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Parvovirus B19 Infection Studies
- Pneumonia and Respiratory Infections
- Facial Nerve Paralysis Treatment and Research
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Antenna Design and Analysis
- Genital Health and Disease
- Influenza Virus Research Studies
University of Helsinki
2016-2025
Helsinki University Hospital
2016-2025
Sydney Local Health District
2023
University of Ljubljana
2023
University Medical Center Hamburg-Eppendorf
2023
Universität Hamburg
2023
Hospital District of Helsinki and Uusimaa
2010
Borealis (Finland)
2009
North Karelia Central Hospital
1998
The first case of coronavirus disease (COVID-19) in Finland was confirmed on 29 January 2020. No secondary cases were detected. We describe the clinical picture and laboratory findings 3-23 days since symptoms. SARS-CoV-2/Finland/1/2020 virus strain isolated, genome showing a single nucleotide substitution to reference from Wuhan. Neutralising antibody response appeared within 9 along with specific IgM IgG response, targeting particularly nucleocapsid spike proteins.
Antibody-screening methods to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) need be validated. We evaluated SARS-CoV-2 IgG and IgA ELISAs in conjunction with the EUROLabworkstation (Euroimmun, Lübeck, Germany). Overall specificities were 91.9% 73.0% for ELISAs, respectively. Of 39 disease patients, 13 positive 11 alone at sampling. IgGs IgAs respectively detected a median of 12 days after symptom onset.
Background Understanding the false negative rates of SARS-CoV-2 RT-PCR testing is pivotal for management COVID-19 pandemic and it has implications patient management. Our aim was to determine real-life clinical sensitivity RT-PCR. Methods This population-based retrospective study conducted in March–April 2020 Helsinki Capital Region, Finland. Adults who were clinically suspected infection underwent testing, with sufficient data their medical records grading suspicion eligible. In addition...
Central nervous system (CNS) infections such as meningitis and encephalitis are life-threatening conditions that demand hospital care prompt identification of the causative agent. Since 2015, there has been only one CE-IVD-marked rapid multiplexed diagnostic assay in cassette format for bacterial viral detection from cerebrospinal fluid (CSF): BioFire FilmArray meningitis/encephalitis (ME) panel. In beginning 2022, Qiagen introduced QIAstat-Dx It is a multiplex PCR test intended suspected...
JC polyomavirus (JCPyV) persists asymptomatic in more than half of the human population. Immunocompromising conditions may cause reactivation and acquisition neurotropic rearrangements viral genome, especially non-coding control region (NCCR). Such rearranged JCPyV strains are strongly associated with development progressive multifocal leukoencephalopathy (PML). Using next-generation sequencing (NGS) bioinformatics tools, NCCR was characterized cerebrospinal fluid (CSF; N=21) brain tissue...
To assess the diagnostic potential of polymerase chain reaction (PCR) in herpes simplex virus (HSV) encephalitis.Samples CSF from 516 patients with encephalitis were studied for HSV-DNA by PCR.Samples taken one to 29 days onset symptoms 38 (7.4%) positive, 32 (6.2%) HSV-1 and six (1.2%) HSV-2. At follow up, eight 28 still HSV-PCR positive. A serum:CSF antibody ratio HSV but not other viruses was detected 25 positive thus supporting initial PCR findings. Patients concentrated age group > or =...
In Finland, the first infections caused by 2009 pandemic influenza A(H1N1) virus were identified on May 10. During next three months almost all found from patients who had recently traveled abroad. September started to spread in general population, leading localized outbreaks and peak epidemic activity was reached during weeks 43-48.The nucleotide sequences of hemagglutinin (HA) neuraminidase (NA) genes viruses collected 138 determined. The analyzed represented mild severe different...
We report an adenovirus outbreak with unusually severe clinical presentation, particularly in military conscripts and their close contacts. During 1 February–30 June 2024, 129 patients infection were hospitalised, 30 admitted to ICU, 10 required ECMO treatment six died. Cases consisted of 75 (58.1%) 54 civilians (41.9%). Most samples type 7 (97/108; 89.8%); all 24 sequenced subtype 7d. August–30 November 274 additional hospitalised cases identified from registries.
Human herpesvirus 6 DNA was detected by PCR in the tear fluid of 7 (35%) 20 patients with Bell's palsy and 1 (5%) healthy controls. Varicella-zoster virus 2 but none fluids from These findings suggest an association between human herpesviruses palsy.
Abstract Cytomegalovirus (CMV) replication in organ transplant recipients is commonly diagnosed by quantitative PCR methods. However, there has been a poor inter‐laboratory correlation of viral load values due to the lack an international reference standard. In recent study, COBAS® AmpliPrep/COBAS® TaqMan® (CAP/CTM) CMV test calibrated 1st WHO standard, showed good reproducibility across multiple laboratories. Fifty‐seven follow‐up plasma specimens from 10 kidney with were examined using new...
The aim of this study was to improve detection Mycoplasma pneumoniae and Chlamydia in clinical specimens by developing a multiplex real-time PCR assay that includes identification macrolide-resistant M. pneumoniae. Novel assays targeting conserved hypothetical protein gene, 23S rRNA gene mutations associated with macrolide resistance human β-globin (an endogenous internal control) were designed combined previously published C. ompA gene. resulting quadraplex validated panel supplemented...
This study characterizes a large Mycoplasma pneumoniae outbreak observed in Kymenlaakso Southeastern Finland during August 2017-January 2018. The first part of the investigation included 327 patients, who sought healthcare consultation at local GPs or hospitals due to clinical symptoms, and were tested for M. antibodies (Patient cohort). second investigation, conducted approximately 4 weeks after peak outbreak, consisted school screening pupils (N = 239) three different buildings by PCR on...
In 2019, more than 200 cases of Chlamydia trachomatis negative/equivocal by the Aptima Combo 2 assay (AC2, target: 23S rRNA) with slightly elevated relative light units (RLUs), but positive (ACT, 16S have been detected in Finland To identify cause AC2 CT false-negative specimens, we sequenced parts rRNA gene 40 specimens that were ACT positive. A single nucleotide polymorphism (SNP; C1515T C. gene) was revealed 39 AC2/ACT discordant specimens. No decrease number mandatorily notified observed...
The aim was to examine the prevalence of Mycoplasma genitalium and determine mutations leading resistance macrolides fluoroquinolones in a sexually transmitted infection clinic setting Finland, as service evaluation, validate performance commercial Aptima® assay. Urogenital samples were studied for M. with an automated assay on Panther® system (Hologic), in-house real-time polymerase chain reaction (PCR) (mgpB). Positive specimens further associated macrolide within 23S rRNA gene known...
Mitigation of the ongoing coronavirus disease 2019 (COVID-19) pandemic requires reliable and accessible laboratory diagnostic services. In this study, performance one laboratory-developed test (LDT) two commercial tests, cobas SARS-CoV-2 (Roche) Amplidiag COVID-19 (Mobidiag), were evaluated for detection severe acute respiratory syndrome 2 (SARS-CoV-2) RNA in specimens. A total 183 specimens collected from suspected patients studied with all three methods to compare their performance....
A 23-month-old girl died after 2 days' illness with rash, fever, and convulsions. Neuropathologic findings were consistent viral hemorrhagic encephalitis in pontine tegmentum medial thalamic areas. Human herpesvirus 6 (HHV-6) DNA was detected nuclei by situ hybridization. In addition, polymerase chain reaction for HHV-6 of serum paraffin-embedded tissue positive, serology indicated an acute primary infection. This is the first case showing brain cells immunocompetent patient encephalitis.