Extracellular vesicles are released by various cell types, particularly tumor cells, and may be potential targets for blood-based cancer diagnosis. However, studies performed on blood-borne to date have been limited lack of effective, standardized purification strategies. Using in situ prepared nanoporous membranes, we present a simple strategy employing microfluidic filtration system isolate from whole blood samples. This method can applied purify nano-sized particles allowing isolation...

Artificial exosomes of ~100 nm diameter, enclosed with lipid bilayers, are fabricated from living cells and transfer biological signal components such as encapsulated RNAs proteins, plasma membrane or both.

We propose a microfluidic system that generates nanovesicles (NVs) by slicing living cell membrane with microfabricated 500 nm-thick silicon nitride (SixNy) blades. Living cells were sliced the blades while flowing through microchannels lined Plasma fragments from self-assembled into spherical NVs of ~100-300 nm in diameter. During self-assembly, plasma enveloped exogenous materials (here, polystyrene latex beads) buffer solution. About 30% beads encapsulated NVs, and generated delivered...

We present a simple and rapid method to isolate extracellular vesicles (EVs) by using polyethylene glycol/dextran aqueous two-phase system (ATPS). This isolated more than ~75% of melanoma-derived EVs from mixture serum proteins. To increase the purity EVs, batch procedure was combined as additional steps remove protein contaminants, removed ~95% contaminants. also performed RT-PCR western blotting verify diagnostic applicability detected mRNA derived melanoma cells CD81 in EVs.

Abstract On‐skin healthcare patch‐type devices have great technological challenges in monitoring full‐day activities and wearing for multiple days without detachment. These can be overcome when the sensor is air permeable but waterproof. This study presents a light‐weight, highly stable, stretchable Au electrode that fabricated by sputtering on an imidized nanofiber mat. The contact surface of hydro‐wetting outer hydrophobic, so porous simultaneously has excellent sweat permeability...

Abstract In implantable bioelectronics, which aim for semipermanent use of devices, biosafe energy sources and packaging materials to protect devices are essential elements. However, research so far has been conducted in a direction where they cannot coexist. Here, the development capacitance‐matched triboelectric implants driven is reported by ultrasound under 500 mW cm −2 safe intensity realize battery‐free, miniatured, wireless neurostimulator with full titanium (Ti) packaging. The...

A new polar ammonium tungsten tellurite, (NH4)2Te2WO8, has been synthesized and structurally characterized. The material was hydrothermally, using NH4OH, WO3, TeO2 as reagents, characterized by single-crystal X-ray diffraction. (NH4)2Te2WO8 crystallizes in the noncentrosymmetric (NCS) monoclinic space group P21 (No. 4) with a = 6.9716(9) Å, b 7.0279(9) c 9.4593(13) β 99.188(2)°. exhibits two-dimensional structure consisting of WO6 octahedra connected to TeO4 polyhedra. Both W6+ Te4+ cations...

Recently, the number of patients infected by drug-resistant pathogenic microbes has increased remarkably worldwide, and a studies have reported new antibiotics from natural sources. Among them, chitosan, with high molecular weight α-conformation, exhibits potent antimicrobial activity, but useful applications as an antibiotic are limited its cytotoxicity insolubility at physiological pH. In present study, antibacterial activity low water-soluble (LMWS) α-chitosan (α1k, α5k, α10k masses 1, 5,...

The combination of hMSCs with 3D scaffolds has become an important approach to creating functional bone constructs. Bioreactors are tools mitigate mass transfer limitations and provide controlled physiochemical biomechanical environments for the construct development. Media flow in bioreactor systems is generally either parallel or transverse respect construct, different cellular microenvironments In this study, a custom designed modular perfusion system was operated under flow. influence...

In this paper, a hybrid-type converter, which can overcome the drawbacks of conventional phase-shifted full-bridge (PSFB) such as narrow zero-voltage-switching range, conduction loss by circulating current, and large output filter inductor, is analyzed evaluated. The converter discussed in paper consists PSFB with rectifier half-bridge LLC series-resonant (SRC) voltage-doubler (VDR), sharing lagging-leg switches integrating VDR SRC into node secondary-side converter. This structure allows...

The use of autologous nerve grafts remains the gold standard for treating defects, but current repair techniques are limited by donor tissue availability and morbidity associated with loss. Recently, conduits in injury repair, made possible engineering, has shown therapeutic potential. We manufactured a biodegradable, collagen-based conduit containing decellularized sciatic matrix compared this silicone peripheral regeneration using rat model. contains growth factor, brain-derived...

Implantable medical devices (IMDs) enable patients to monitor their health anytime and receive treatment anywhere. However, due the limited capacity of a battery, functionalities are restricted, may not achieve intended potential fully. The most promising way solve this problem is wireless power transfer (WPT) technology. In study, WPT based implantable electrocardiogram (ECG) monitoring device that continuously records ECG data has been proposed, its effectiveness verified through an animal...

Sodium nitroprusside is a nitric oxide donor involved in the regulation of motility, hyperactivation, capacitation, and acrosome reaction (AR) spermatozoa. However, molecular mechanism underlying this has not yet been elucidated. Therefore, study was designed to evaluate basis for effects sodium on different processes spermatozoa its consequences subsequent oocyte fertilization embryo development. In vitro study, mouse were incubated with various concentrations (1, 10, 100 μM) 90 min. Our...

Since robust osteogenic differentiation and mineralization are integral to the engineering of bone constructs, understanding impact cellular microenvironments on human mesenchymal stem cell (hMSCs) is crucial optimize bioreactor strategy. Two perfusion flow conditions were utilized in order understand configuration hMSC construct development during both pre-culture (PC) growth media its subsequent induction (OI). The in-house was controlled perfuse either around (termed parallel [PF])...

Extracellular vesicles can enhance cell proliferation by stimulating signal transduction and delivering genetic materials, thus may have applications in regenerative medicine other therapeutic applications. The processes employed to isolate extracellular vesicles, however, are complex achieve low yield. To overcome these obstacles, a large-scale, micropore device for generating vesicle-mimetic nanovesicles that characteristics similar those of is fabricated. generated through the...

Wireless power transfer systems are increasingly used as a means of charging implantable medical devices. However, the heat or thermal radiation from wireless system can be harmful to biological tissue. In this research, we designed and implemented system-based device with low radiation, achieving 44.5% coil-to-coil efficiency. To suppress transmitting coil during charging, minimized ESR value coil. increase efficiency, ferrite film was applied on receiving part. Based analyses, fabricated...

This paper presents the novel control method of Four-Switch Buck-Boost (FSBB) converter for Super Capacitor Pre-Charger (SCPC) in hydrogen electric vehicles. The is proposed to improve voltage regulation characteristic during super capacitor pre-charging, and able maintain continuity duty ratio conversion ratio. Even though operation mode changes achieving unity gain, additional switch state diminishes fluctuation at transition point compared conventional method. Also, size passive...

This paper proposes a novel soft-switched auxiliary resonant circuit to provide zero-voltage transition at turn on for conventional pulsewidth-modulated boost converter in power factor correction (PFC) application. The proposed enables main switch of the under switching condition and simultaneously achieves both off. Moreover, purpose an intelligent multichip module fabrication, is designed satisfy several design constraints, including space saving, low cost, easy fabrication. As result,...

For the first time, under actual three‐phase 765 kV power transmission lines, electric field energy is harvested and Zigbee‐based sensor node powered by operated autonomously. A conductor tube placed between lines earth surface for harvesting. The voltage difference rectified stored in a storage capacitor. self‐sustaining management circuit adopting low‐power comparator with hysteresis devised to monitor operate autonomously according energy. Experimental results show that data transfer...