A5085357988- Sinusitis and nasal conditions
- Cancer, Hypoxia, and Metabolism
- Obstructive Sleep Apnea Research
- Asthma and respiratory diseases
- Epigenetics and DNA Methylation
- Allergic Rhinitis and Sensitization
- Advanced Chemical Sensor Technologies
- Head and Neck Surgical Oncology
- Extracellular vesicles in disease
- Analytical Chemistry and Sensors
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Gas Sensing Nanomaterials and Sensors
- Neuroscience of respiration and sleep
- Cancer Research and Treatments
- RNA modifications and cancer
- MicroRNA in disease regulation
- Nasal Surgery and Airway Studies
- Infectious Diseases and Mycology
- Cancer, Stress, Anesthesia, and Immune Response
- High Altitude and Hypoxia
- Engineering Applied Research
- Eicosanoids and Hypertension Pharmacology
- Cancer Genomics and Diagnostics
- Oral and Maxillofacial Pathology
Seoul National University
2016-2025
Korea Innotech (South Korea)
2023-2025
Seoul National University Hospital
2009-2024
Pohang University of Science and Technology
2015-2024
Sungkyunkwan University
2018-2023
Yeouido St. Mary's Hospital
2023
Catholic University of Korea
2023
Kangbuk Samsung Hospital
2018-2023
New Generation University College
2015-2021
National University College
2016-2020
Abstract Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne have been hindered by the lack of purification strategies. In this study, efficient EV isolation method was developed using polyethylene glycol/dextran aqueous two phase system (ATPS). This provides high...
Nasal polyposis implies a refractory clinical course in case of chronic rhinosinusitis (CRS). Although hypoxia is believed to be associated with nasal polyposis, little known about the mechanism underlying polypogenesis.To determine if drives by epithelial-to-mesenchymal transition (EMT).Immunoblotting, immunofluorescence, flow cytometry, and real-time polymerase chain reaction were performed evaluate EMT hypoxic markers human epithelial cells (hNECs) sinonasal tissues from patients CRS or...
Abstract Extracellular vesicles (EVs) may play an important role in cancer development and progression. We aimed to investigate the prognostic potential of prostate-specific EVs prostate (PCa) patients. Plasma tissue were collected from patients who underwent surgery for PCa (n = 82) or benign prostatic hyperplasia (BPH, n 28). To analyze quantity prostate, we performed transmission electron microscopy (TEM), immuno-TEM with CD63 membrane antigen (PSMA), immunofluorescence staining. After EV...
Background Studies on the pathophysiology of nasal polyps in human subjects have been limited; thus an animal model is needed. There increasing evidence supporting role Staphylococcus aureus enterotoxin B (SEB) pathogenesis polyposis. The aim this study was to investigate histological and immunologic effects SEB formation polypoid lesions allergic rhinosinusitis murine model. Methods After induction ovalbumin (OVA)-induced rhinosinusitis, OVA with (5 or 500 ng) instilled into cavity mice for...
Abstract Metformin is a first-line drug for treating type 2 diabetes. Although metformin known to phosphorylate AMP-activated protein kinase (AMPK), it unclear how the glucose-lowering effect of related AMPK activation. The aim this study was identify urinary endogenous metabolites affected by and novel underlying molecular mechanisms its anti-diabetic effect. Fourteen healthy male subjects were orally administered (1000 mg) once. First morning urine samples taken before after administration...
Abstract Despite many efforts to develop hormone therapy and chemotherapy, no effective strategy suppress prostate cancer metastasis has been established because the is not well understood. We here investigate a role of CBP/p300-interacting transactivator with E/D-rich carboxy-terminal domain-2 (CITED2) in metastasis. CITED2 highly expressed metastatic cancer, its expression correlated poor survival. The gene activated by ETS-related that overexpressed due chromosomal translocation. acts as...
We present a simple and rapid method to isolate extracellular vesicles (EVs) by using polyethylene glycol/dextran aqueous two-phase system (ATPS). This isolated more than ~75% of melanoma-derived EVs from mixture serum proteins. To increase the purity EVs, batch procedure was combined as additional steps remove protein contaminants, removed ~95% contaminants. also performed RT-PCR western blotting verify diagnostic applicability detected mRNA derived melanoma cells CD81 in EVs.
MicroRNAs (miRNAs) of urine exosomes have emerged as biomarkers for urological cancers, owing to their high stability. MiRNAs been linked factors associated with aggressive prostate cancer such biochemical recurrence (BCR) and metastasis. In this study, we aimed identify urinary exosomal miRNAs prognostic markers BCR in intermediate-risk cancer. We profiled the expression levels via next generation sequencing from 21 non-BCR patients 6 A total were found be differentially expressed (>...
Despite the worldwide effect of coronavirus disease 2019 (COVID-19) pandemic, underlying mechanisms fatal viral pneumonia remain elusive. Here, we show that critical COVID-19 is associated with enhanced eosinophil-mediated inflammation when compared to non-critical cases. In addition, confirm increased T helper (Th)2-biased adaptive immune responses, accompanying overt complement activation, in group. Moreover, antibody responses and activation are pathogenesis as evidenced by formation...
This study investigated the clinical implications of neutrophil extracellular trap (NET) formation (NETosis) and eosinophil (EET) (EETosis) regarding refractoriness in chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP).Nasal polyp specimens were obtained from 117 patients CRSwNP who received endoscopic sinus surgery. Disease control status at postoperative 1 year was assessed. Refractory cases defined as partly controlled or uncontrolled according to EPOS 2020 guidelines. NETosis...
Background The enhancement of cell motility is a critical event during tumor spreading. Since myosin light chain kinase (MLCK) regulates behavior, it regarded as promising target in terms preventing invasion and metastasis. MLCK was identified to be associated with human arrest defective-1 (hARD1) through yeast two-hybrid screening, we here tested the possibility that hARD1 acts regulator by so doing controls motility. Methodology/Principal Findings physical interaction between confirmed...
Chronic rhinosinusitis (CRS) is a heterogeneous and multifactorial inflammatory disease characterized by involvement of diverse types cells. Asian CRS patients frequently show infiltration neutrophils an elevated level interferon (IFN)-γ; contrast, western exhibit eosinophil enhanced levels Th2-related cytokines. Neutrophilia in tissues decreases sensitivity to corticosteroids, but the mechanisms underlying progression neutrophilic are unclear. In this study, we investigated role IFN-γ with...
The prolyl hydroxylase domain-containing proteins (PHD1-3) and the asparaginyl hydroxlyase factor inhibiting HIF (FIH) are oxygen sensors for hypoxia-inducible factor-driven transcription of hypoxia-induced genes, but whether these affect oxygen-dependent epigenetic regulation more broadly is not known. Here, we show that FIH exerts an additional role as sensor in control by histone lysine methyltransferases G9a GLP. hydroxylated inhibited GLP under normoxia. When reaction was limited...
The endocranial morphology of the skull base is an important factor in determining craniofacial asymmetry. Moreover, although patency cranial suture would be first factor, unicoronal, unilambdoid synostosis and deformational plagiocephaly can also differentiated by means axis angulation anterior posterior fossa. These findings indicate importance authors hypothesized that distraction might cause stress on could modify deformities.This study compared technique with traditional bone...
Abstract Background Since the recent establishment of a murine model eosinophilic chronic rhinosinusitis with nasal polyps ( CRS w NP ), both development new drugs for treatment or prevention and elucidation their pathogenesis have been feasible. We investigated therapeutic effects resveratrol on its mechanism action using model. Methods After induction , were tested compared those triamcinolone acetonide. Histopathologic changes evaluated hematoxylin eosin overall inflammation, Sirius red...