A5006080567- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Sepsis Diagnosis and Treatment
- Luminescence Properties of Advanced Materials
- COVID-19 Clinical Research Studies
- Helicobacter pylori-related gastroenterology studies
- Cancer, Lipids, and Metabolism
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Seaweed-derived Bioactive Compounds
- Cancer-related gene regulation
- COVID-19 diagnosis using AI
- Bacterial Identification and Susceptibility Testing
- Pharmacological Effects of Natural Compounds
- Clostridium difficile and Clostridium perfringens research
- Bladder and Urothelial Cancer Treatments
- Fungal Infections and Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Nosocomial Infections in ICU
- Phytochemicals and Antioxidant Activities
- Antibiotic Resistance in Bacteria
- Gastrointestinal disorders and treatments
- Cholesterol and Lipid Metabolism
- Gut microbiota and health
Yonsei University
2016-2025
Incheon National University
2021-2025
George Mason University
2025
Gangnam Severance Hospital
2014-2024
Soongsil University
2024
Seoul National University Hospital
2009-2024
Sanofi (South Korea)
2021-2022
Severance Hospital
2018
Gachon University Gil Medical Center
2014-2015
Gachon University
2014
We have investigated the cytoprotective effect of eckol, which was isolated from Ecklonia cava , against oxidative stress induced cell damage in Chinese hamster lung fibroblast (V79‐4) cells. Eckol found to scavenge 1,1‐diphenyl‐2‐picrylhydrazyl radical, hydrogen peroxide (H 2 O ), hydroxy intracellular reactive oxygen species (ROS), and thus prevented lipid peroxidation. As a result, eckol reduced H death V79‐4 In addition, inhibited by serum starvation radiation scavenging ROS. increase...
Both p53 and the Wnt signaling pathway play important roles in regulating differentiation of mouse embryonic stem cells (mESCs). However, it is not known whether they directly and/or functionally crosstalk mESCs. Here we report a surprising antidifferentiation function mESCs through pathway. A chromatin-immunoprecipitation-based microarray (ChIP-chip) gene expression assays reveal that significantly (P value, 0.000048) overrepresented p53-regulated genes The five ligand robustly induced by...
A red-emitting phosphor, CaY1-xEuxAl3O7 (CYA:Eu3+), was prepared by conventional solid-state reaction, and its structural properties were investigated means of Rietveld refinement method maximum entropy using an X-ray source. XRD patterns confirm the tetragonal phase CYA:Eu3+ phosphors. The photoluminescence samples for application to white light-emitting diodes (WLEDs). emission spectrum is dominated a red peak located at 616 nm due 5D0 → 7F2 electric dipole transition Eu3+ ions....
We assessed the safety and immunogenicity of two recombinant DNA vaccines for COVID-19: GX-19 containing plasmid encoding SARS-CoV-2 spike protein, GX-19N receptor-binding domain (RBD) foldon, nucleocapsid protein.Two open-label non-randomised phase 1 trials, one other were done at hospitals in South Korea. enrolled healthy adults aged 19-49 years trial 19-54 trial. Participants who tested positive by serological testing excluded. At 4-week intervals, participants received vaccine doses...
Abstract We investigated the cytoprotective effect of phloroglucinol, which was isolated from Ecklonia cava (brown alga), against oxidative stress induced cell damage in Chinese hamster lung fibroblast (V79‐4) cells. Phloroglucinol found to scavenge 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical, hydrogen peroxide (H 2 O ), hydroxy intracellular reactive oxygen species (ROS), and thus prevented lipid peroxidation. As a result, phloroglucinol reduced H apoptotic cells formation V79‐4 In...
Runt-related transcription factor 2 (Runx2) transactivates many genes required for osteoblast differentiation. The role of N-α-acetyltransferase 10 (NAA10, arrest-defective-1), originally identified in yeast, remains poorly understood mammals. Here we report a new NAA10 function Runx2-mediated osteogenesis. Runx2 stabilizes osteoblasts during BMP-2-induced differentiation, and turn controls this differentiation by inhibiting Runx2. delays bone healing rat calvarial defect model development...
To gain further insights into the biological functions of Dna2, previously known as a cellular replicative helicase in Saccharomyces cerevisiae, we examined biochemical properties recombinant Dna2 protein purified to homogeneity. Besides single-stranded (ss) DNA-dependent ATPase activity reported previously, were able demonstrate that ssDNA-specific endonuclease is intrinsically associated with Dna2. Moreover, was capable degrading duplex DNA an ATP-dependent fashion. ATP and dATP, only...
AbstractIn the present study, triphlorethol-A, a phlorotannin, was isolated from Ecklonia cava and its antioxidant properties were investigated. Triphlorethol-A found to scavenge intracellular reactive oxygen species (ROS) 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, thus prevented lipid peroxidation. The radical scavenging activity of triphlorethol-A protected Chinese hamster lung fibroblast (V79-4) cells exposed hydrogen peroxide (H2O2) against cell death, via activation ERK protein....
Triphlorethol‐A, phlorotannin found in Ecklonia cava , induced heme oxygenase‐1 (HO‐1) expression at mRNA and protein levels, leading to increased HO‐1 activity. Transcription factor NF‐E2 related 2 (Nrf2) regulates antioxidant response element (ARE) of phase detoxifying enzymes. Triphlorethol‐A nuclear translocation, ARE binding, transcriptional activity Nrf2. exhibited activation ERK U0126, inhibitor kinase, suppressed triphlorethol‐A Nrf2, finally decreased level. Taken together, these...
Background The enhancement of cell motility is a critical event during tumor spreading. Since myosin light chain kinase (MLCK) regulates behavior, it regarded as promising target in terms preventing invasion and metastasis. MLCK was identified to be associated with human arrest defective-1 (hARD1) through yeast two-hybrid screening, we here tested the possibility that hARD1 acts regulator by so doing controls motility. Methodology/Principal Findings physical interaction between confirmed...
Purpose: The present study aimed to evaluate the effect of central line (CL) bundle compliance on line-associated bloodstream infections (CLABSIs) in different departments same hospital, including intensive care unit (ICU) and other departments. Materials Methods:The four components CL were hand hygiene, use maximal sterile barrier precautions, chlorhexidine use, selection an appropriate site for venous access.Compliance CLABSIs measured every department [emergency room (ER), ICU, general...
In this report, we investigated the phenotypes caused by temperature-sensitive (ts) mutant alleles of dna2(+) Schizosaccharomyces pombe, a homologue DNA2 budding yeast, in an attempt to further define its function vivo with respect lagging-strand synthesis during S-phase cell cycle. At restrictive temperature, dna2 cells arrested at late but were unaffected bulk DNA synthesis. Moreover, they exhibited aberrant mitosis when combined checkpoint mutations, keeping role for Dna2 Okazaki fragment...
The tumor suppressor p53 is a critical regulator of apoptosis and cell cycle arrest/pro-survival. Upon DNA damage, evokes both arrest/pro-survival transcriptional programs. ultimate cellular outcome depends on the balance these two However, downstream targets that mediate this fate decision remain to be identified. Using an integrative genomic approach, we identify Rap2b as conserved p53-activated gene counters p53-mediated after damage. directly binds promoter activates its transcription....
Elevated lipid metabolism promotes cancer cell proliferation. Clear renal carcinoma (ccRCC) is the most common subtype of kidney cancers, characterized by ectopic accumulation. However, relationship between aberrant and tumorigenesis in ccRCC not thoroughly understood. Here, we demonstrate that ring finger protein 20 (RNF20) acts as a tumor suppressor ccRCC. RNF20 overexpression repressed lipogenesis proliferation inhibiting sterol regulatory element-binding 1c (SREBP1c), SREBP1 suppression,...
Histone modifications on major transcription factor target genes are one of the regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and known to demethylate histone H3K9, repressive gene marker. To better understand function PHF2 in adipocyte differentiation, we constructed stable knock-down cells by using mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, showed reduced lipid accumulation compared...
We examined the anti-cancer effects and molecular mechanism of simvastatin in human castration-resistant prostate cancer (CRPC) cells, particularly focused on LIN28B its target molecule, let-7 microRNA (miRNA) among various genes NF-κB. A CRPC cell line (PC3) was used current study. Gene expression patterns were evaluated using real time-PCR western blot analysis. CCK-8 assay for assessing viability proliferation, a clonogenic adopted to evaluate clonal proliferative capabilities. Induction...
Background A latent cytomegalovirus (CMV) cause chronic inflammation through undesirable inflation of cell-mediated immune response. CMV immunoglobulin G has been associated with cardiovascular disease and type 1 diabetes mellitus. We evaluated impact diseases on new-onset 2 mellitus (T2DM). Methods From the Korean Health Insurance Review Assessment Service claim database entire population 50 million, we retrieved 576 adult case group diagnosed International Statistical Classification...