A5026297073- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Prostate Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Regulation of Appetite and Obesity
- Atherosclerosis and Cardiovascular Diseases
- Gene expression and cancer classification
- Lung Cancer Treatments and Mutations
- Renal cell carcinoma treatment
- Sphingolipid Metabolism and Signaling
- Gastric Cancer Management and Outcomes
- PARP inhibition in cancer therapy
- Machine Learning in Bioinformatics
- Gallbladder and Bile Duct Disorders
- Single-cell and spatial transcriptomics
Seoul National University
2017-2024
Weatherford College
2023
The osteochondrogenic switch of vascular smooth muscle cells (VSMCs) is a pivotal cellular process in atherosclerotic calcification. However, the exact molecular mechanism transition VSMCs remains to be elucidated. Here, we explore regulatory role TXNIP (thioredoxin-interacting protein) phenotypical transitioning toward responsible for calcification.The phenotypes Txnip-/- mice were analyzed combination with single-cell RNA-sequencing. Tagln-Cre; Txnipflox/flox (smooth cell-specific Txnip...
Purpose There have been needs to improve the sensitivity of liquid biopsy. This report aims analytical and clinical validation a next-generation sequencing (NGS)–based circulating tumor DNA (ctDNA) assay.Materials Methods Analytical was conducted in vitro by evaluating limit detection (LOD), precision, specificity for various genomic aberrations. The real-world performance non–small cell lung cancer (NSCLC) assessed comparing results AlphaLiquid100 tissue-based results.Results LODs with 30...
98 Background: Accurate detection of mutations in HRR pathway genes among patients with mCRPC is essential for selecting who would benefit poly (ADP-ribsose) polymerase (PARP) inhibitor therapies. Traditional tissue-based methods can be associated practical challenges as acquisition specimens involve significant risk or high costs. For prostate cancer patients, obtaining adequate tissue samples even more difficult, considering the unique characteristics disease. This study aimed to evaluate...
This study compared plasma cell-free DNA (cfDNA) and tumor tissue (ttDNA) to explore the clinical applicability of cfDNA in patients with metastatic gastric cancer (mGC) receiving palliative second-line paclitaxel + ramucirumab treatment. Targeted sequencing 106 genes was conducted using germline at baseline (baseline-cfDNA) progressive disease (PD-cfDNA). The results were those ttDNA-based panel data. Of 76 consecutive patients, 46 (27 males; median age 57.5 [range, 32-73] years) who had...
This study aimed to identify mutation profile similarities between tissue and circulating tumor DNA (ctDNA) explore driver mutations as potential prognostic or predictive biomarkers druggable targets in patients with advanced biliary tract cancer (BTC). We prospectively enrolled 18 BTC analyzed next-generation sequencing data from 60 ctDNA samples using AlphaLiquid® 100. assay screens up 118 genes for single-nucleotide variants (SNVs) insertion deletions (INDELs), 27 copy number alterations...
Traditional tissue-based assessments of genomic alterations in castration-resistant prostate cancer (CRPC) can be challenging. To evaluate the real-world clinical utility liquid biopsies for evaluation CRPC, we preemptively collected available plasma samples and archival tissue from patients that were being treated clinically confirmed CRPC. The cell-free DNA (cfDNA) tumor analyzed using AlphaLiquid®100-HRR panel. Plasma a total 87 included this study. Somatic mutations cfDNA detected 78...
Studies have indicated that weight regain following loss predisposes obese individuals to metabolic disorders; however, the molecular mechanism of this potential adverse effect is not fully understood. Here we investigated global transcriptome changes and immune response in mouse white adipose tissue caused by regain. We established a diet switch protocol compare effects with those gain without precedent loss, maintenance chow diet. conducted time course analysis gonadal (gWAT) during...
Abstract PDGFRA+ cells have been identified as adipocyte stem (ASCs) that differentiate into beige adipocytes in white adipose tissue (WAT) following thermogenic stimuli. To elucidate the molecular heterogeneity of ASCs, we conducted single-cell transcriptomic profiling isolated from inguinal WAT (iWAT) mice treated with beta3 adrenergic receptor agonist CL316243. Single-cell RNA-seq revealed nine major clusters, which were categorized four groups: resting, proliferating, differentiating,...
Abstract Background: Plasma cell-free DNA (cfDNA) analysis has emerged as an appealing alternative to detect somatic mutations. This study compared cfDNA at baseline (baseline-cfDNA) and progressive disease (PD-cfDNA) tumor tissue (ttDNA) in patients with metastatic gastric cancer who underwent palliative second-line paclitaxel+ramucirumab treatment. Methods: We conducted targeted sequencing of 106 cancer-related genes using germline DNA, baseline-cfDNA, PD-cfDNA samples. The results were...
Abstract Background: Assessment of genomic alterations in castration-resistant prostate cancer (CRPC) patients has become an important process for treatment decisions, but traditional tissue-based evaluation can be challenging. Methods: To evaluate the clinical utility liquid biopsy CRPC, we have prospectively collected plasma samples from being treated clinically confirmed CRPC. The cell-free DNA (cfDNA) extracted were analyzed using AlphaLiquid®100-HRR panel. Available archival tumor...
Genetically engineered mouse models are used in high-throughput phenotyping screens to understand genotype-phenotype associations and their relevance human diseases. However, not all mutant lines with detectable phenotypes associated Here, we propose the "Target gene selection system for models" (TarGo). Using a combination of disease descriptions, network topology, correlations, novel genes that potentially related diseases suggested. We constructed interaction using protein-protein...