A5075390964- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- interferon and immune responses
- Virus-based gene therapy research
- Edible Oils Quality and Analysis
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- HIV Research and Treatment
- Virology and Viral Diseases
- RNA regulation and disease
- Viral Infections and Immunology Research
- Cytomegalovirus and herpesvirus research
- Autophagy in Disease and Therapy
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Immune Response and Inflammation
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- Enzyme Catalysis and Immobilization
- Cholesterol and Lipid Metabolism
- Food Chemistry and Fat Analysis
- Animal Disease Management and Epidemiology
Henan Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Ministry of Education of the People's Republic of China
2024
Henan University
2024
Henan Institute of Education
2022-2023
Education Department of Henan Province
2022-2023
Shanghai Institutes for Biological Sciences
2009-2019
Chinese Academy of Sciences
2009-2019
Center for Excellence in Molecular Cell Science
2009-2019
Abertay University
2019
Chromatin dynamics regulated by epigenetic modification is crucial in genome stability and gene expression. Various mechanisms have been identified the pathogenesis of human diseases. Here, we examined effects ten agents on pseudorabies virus (PRV) infection using GFP-reporter assays. Inhibitors bromodomain protein 4 (BRD4), which receives much more attention cancer than viral infection, was found to exhibit substantial anti-viral activity against PRV as well a range DNA RNA viruses. We...
Alphaherpesvirus infection results in severe health consequences a wide range of hosts. USPs are the largest subfamily deubiquitinating enzymes that play critical roles immunity and other cellular functions. To investigate role alphaherpesvirus replication, we assessed 13 USP inhibitors for PRV replication. Our data showed all tested compounds inhibited with USP14 inhibitor b-AP15 exhibiting most dramatic effect. Ablation also influenced whereas replenishment null cells restored viral...
African swine fever virus (ASFV) is responsible for enormous economic losses in the global industry. The ASFV genome encodes approximate 160 proteins, most of whose functions remain largely unknown. In this study, we examined roles K205R endoplasmic reticulum (ER) stress, autophagy, and inflammation. We observed that was located both cytosolic membrane fractions, formed stress granules cells. Furthermore, triggered ER activated unfolded protein response through activating transcription...
Autophagy maintains cellular homeostasis by degrading organelles, proteins, and lipids in lysosomes. is involved the innate adaptive immune responses to a variety of pathogens. Some viruses can hijack host autophagy enhance their replication. However, role porcine reproductive respiratory syndrome virus (PRRSV) infection unclear. Here, we show that N-Myc downstream-regulated gene 1 (NDRG1) deficiency induced autophagy, which facilitated PRRSV replication regulating lipid metabolism. NDRG1...
Porcine reproductive and respiratory syndrome virus (PRRSV) infection leads to late-term failure illness that affect the global swine industry. Epigallocatechin gallate (EGCG) is a polyphenolic compound from green tea exerts antiviral activity against diverse viruses. This study aimed report an uncharacterized mechanism of how EGCG restricted PRRSV proliferation. showed no significant effects on cell viability, cycle progression, apoptosis in porcine alveolar macrophages MARC-145 cells. The...
Porcine reproductive and respiratory syndrome virus (PRRSV) presents a significant economic concern for the global swine industry due to its connection serious production losses increased mortality rates. There is currently no specific treatment PRRSV. Previously, we had uncovered that PRRSV-activated lipophagy facilitate viral replication. However, precise mechanism PRRSV used trigger autophagy remained unclear. Here, found GP5 enhanced mitochondrial Ca
RAB GTPases (RABs) control intracellular membrane trafficking with high precision. In the present study, we carried out a short hairpin RNA (shRNA) screen focused on library of 62 RABs during infection porcine reproductive and respiratory syndrome virus 2 (PRRSV-2), member family Arteriviridae . We found that 13 negatively affect yield PRRSV-2 progeny virus, whereas 29 have positive impact virus. Further analysis revealed transcriptionally regulated RAB18 through RIG-I/MAVS-mediated...
Viral infection is a significant risk factor for fertility issues. Here, we demonstrated that by neurotropic alphaherpesviruses, such as pseudorabies virus (PRV), could impair female disrupting the hypothalamus-pituitary-ovary axis (HPOA), reducing progesterone (P4) levels, and consequently lowering pregnancy rates. Our study revealed PRV exploited transient receptor potential mucolipin 1 (TRPML1) its lipid activator, phosphatidylinositol 3,5-bisphosphate (PI(3,5)P 2 ), to facilitate viral...
Niemann-Pick C1-like 1 (NPC1L1) plays a critical role in the enterohepatic absorption of free cholesterol. Cellular cholesterol depletion induces transport NPC1L1 from endocytic recycling compartment to plasma membrane (PM), and replenishment causes internalization together with via clathrin-mediated endocytosis. Although has been characterized, other proteins involved are largely unknown. Most vesicular trafficking events dependent on cytoskeleton motor proteins. Here, we investigated roles...
The Niemann-Pick C1 Like 1 (NPC1L1) is a predicted polytopic membrane protein that critical for cholesterol absorption. NPC1L1 takes up free into cells through vesicular endocytosis. Ezetimibe, clinically used absorption inhibitor, blocks the endocytosis of thereby inhibiting uptake. Human 1,332-amino acid with putative sterol-sensing domain (SSD) shows sequence homo-logy to HMG-CoA reductase (HMGCR), (NPC1), and SREBP cleavage-activating (SCAP). Here, we use protease protection...
Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25. The aim this study was to examine antiviral effect CH25H on pseudorabies virus (PRV), swine pathogen that can cause devastating disease and economic losses worldwide. results showed porcine ch25h induced either interferon or PRV infection. infection alveolar macrophages (3D4/21 cells) attenuated overexpression enhanced silencing CH25H....
The M2 isoform of pyruvate kinase (PKM2), which has been identified as the predominant cause Warburg effect in cancer cells, is essential tumor metabolism and growth. However, role PKM2 autophagy remains to be elucidated. present study investigated knockdown on apoptotic cell death human A549 alveolar adenocarcinoma cells. Two short hairpin (sh)RNAs targeting mRNA were designed lentiviral vectors constructed. cells infected with lentiviruses, containing shRNAs against PKM2, expression was...
Pseudorabies virus (PRV) is recognized as the aetiological agent responsible for Aujeszky's disease, or pseudorabies, in swine populations. Rab6, a member of small GTPase family, implicated various membrane trafficking processes, particularly exocytosis regulation. Its involvement PRV infection, however, has not been documented previously. In our study, we observed significant increase Rab6 mRNA and protein levels both PK-15 porcine kidney epithelial cells alveolar macrophages, well lungs...
Interferon-inducible transmembrane proteins (IFITMs) restrict infection by several viruses, such as influenza A virus, West Nile virus and dengue virus. It has not been determined whether porcine IFITMs (pIFITMs) inhibit pseudorabies (PRV), an enveloped, double-stranded DNA which is the etiological agent of Aujeszky's disease in pigs. Here, we report that PRV elicited pIFITM1 expression PK15 kidney epithelial cells 3D4/21 alveolar macrophages. pIFITM2 pIFITM3 was only elevated during...
Emerging viral pathogens cause substantial morbidity and pose a severe threat to health worldwide. However, universal antiviral strategy for producing safe immunogenic inactivated vaccines is lacking. Here, we report an using the novel singlet oxygen (1O2)-generating agent LJ002 inactivate enveloped viruses provide effective protection against infection. Our results demonstrated that efficiently generated 1O2 in solution living cells. Nevertheless, exhibited no signs of acute toxicity vitro...
Pseudorabies virus (PRV) is a double-stranded DNA that causes Aujeszky's disease and responsible for economic loss worldwide. Transmembrane protein 41B (TMEM41B) novel endoplasmic reticulum (ER)-localized regulator of autophagosome biogenesis lipid mobilization; however, the role TMEM41B in regulating PRV replication remains undocumented. In this study, infection was found to upregulate mRNA levels both vitro vivo. For first time, we could be induced by interferon (IFN), suggesting an...
Myostatin (Mstn) is postulated to be a key determinant of muscle loss and cachexia in cancer. However, no experimental evidence supports role for Mstn cancer, particularly regulating the survival growth cancer cells. In this study, we showed that expression was significantly increased different tumor tissues human knockdown inhibited proliferation A knockout (KO) created by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 (CRISPR/Cas9)...