A5031761644- Marine Toxins and Detection Methods
- SARS-CoV-2 and COVID-19 Research
- Marine and coastal ecosystems
- Fungal Infections and Studies
- Forensic Toxicology and Drug Analysis
- Peptidase Inhibition and Analysis
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antifungal resistance and susceptibility
- vaccines and immunoinformatics approaches
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- Biosimilars and Bioanalytical Methods
- Antibiotics Pharmacokinetics and Efficacy
- Click Chemistry and Applications
- Biocrusts and Microbial Ecology
- Opioid Use Disorder Treatment
- Inhalation and Respiratory Drug Delivery
- Cyclopropane Reaction Mechanisms
- Marine Biology and Environmental Chemistry
- Nicotinic Acetylcholine Receptors Study
- Veterinary Pharmacology and Anesthesia
- Animal Ecology and Behavior Studies
- Neuropeptides and Animal Physiology
- HIV/AIDS drug development and treatment
Centers for Disease Control and Prevention
2017-2025
Center for Environmental Health
2020-2025
National Center for Environmental Health
2018-2025
Battelle
2017
University of Alabama at Birmingham
2011-2015
Georgia Institute of Technology
2007
Fentanyl, and the numerous drugs derived from it, are contributing to opioid overdose epidemic currently underway in USA. To identify human exposure these growing public health threats, an LC–MS-MS method for 5 μL dried blood spots (DBS) was developed. This developed detect 3-methylfentanyl, alfentanil, α-methylfentanyl, carfentanil, fentanyl, lofentanil, sufentanil, norcarfentanil, norfentanyl, norlofentanil, norsufentanil, using a separate injection, cyclopropylfentanyl, acrylfentanyl,...
Abstract Health-care workers, laboratorians and overdose prevention centers rely on commercial immunoassays to detect the presence of fentanyl; however, cross-reactivity fentanyl analogs with these kits is largely unknown. To address this, we conducted a pilot study evaluating detection 30 metabolites by 19 commercially available (9 lateral flow assays, 7 heterogeneous 3 homogenous immunoassays). The selected for analysis were compiled from Drug Enforcement Administration National Forensic...
Structural variants of the synthetic opioid fentanyl are a major threat to public health. Following an investigation showing that many derivatives poorly detected by commercial lateral flow and related assays, we created hapten conjugate vaccines using immunogenic virus-like particle carrier eight designed mimic structural features several more dangerous analogues. Immunization mice elicited strong antihapten humoral responses, allowing screening hundreds hapten-specific hybridomas for...
The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest ~ 300 amino acid receptor binding domain (RBD) within S1 subunit spike protein because its key interaction with human angiotensin converting enzyme 2 (hACE2) present on many cell types, especially lung epithelial cells. We report here development functional...
Microcystins (MC) and nodularin (NOD) are toxins released by cyanobacteria during harmful algal blooms. They potent inhibitors of protein phosphatases 1 2A (PP1 PP2A) cause a variety adverse symptoms in humans animals if ingested. More than 250 chemically diverse congeners MCs have been identified, but certified reference materials only available for few. A diagnostic test that does not require each material detection is necessary to identify human exposures. To address this need, our lab...
Microcystins are toxins produced by many cyanobacteria species, which often released into waterways during blue-green algal blooms in freshwater and marine habitats. The consumption of microcystin-contaminated water is a public health concern as these recognized tumor promoters hepatotoxic to humans animals. A method confirm human exposures microcystins needed; therefore, our laboratory has developed an immunocapture liquid chromatography tandem mass spectrometry (LC–MS/MS) targeting the...
SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of respiratory disease COVID-19. Detecting antibodies against virus is an essential tool for tracking infections developing vaccines. Such tests, primarily utilizing enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative value representing quantity specific antibodies). Quantitation vital determining stability decline antibody...
Invasive aspergillosis (IA) resulting from infection by Aspergillus fumigatus is a leading cause of death in immunosuppressed populations. There are limited therapeutic options for this disease and currently no vaccine. evidence that some anti-A. mAbs can provide protection against IA. However, vaccine development has been impeded paucity immunological targets on organism demonstrated to protective responses. Sialylated oligosaccharide epitopes found variety pathogens, including fungi group...
Paralytic shellfish toxins (PSTs), including gonyautoxins and saxitoxins, are produced by multiple species of microalgae dinoflagellates, bioaccumulated other animals. Human exposure to PSTs typically occurs through ingestion recreationally harvested contaminated results in nonspecific symptomology. Confirmation has often relied on the measurement saxitoxin, most toxic congener; however, (GTXs), sulfated carbamate derivatives may be present at higher concentrations. To improve identification...
Microcystins are toxic chemicals generated by certain freshwater cyanobacteria. These can accumulate to dangerous levels during harmful algal blooms. When exposed microcystins, humans at risk of hepatic injury, including liver failure. Here, we describe a method detect microcystins in human plasma using immunocapture followed protein phosphatase inhibition assay. At least 279 have been identified, and most these compounds share common amino acid, the Adda side chain. We targeted this chain...
Abstract The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest ~300 amino acid receptor binding domain (RBD) within S1 subunit spike protein because its key interaction with human angiotensin converting enzyme 2 (hACE2) present on many cell types, especially lung epithelial cells. We report here development...
Abstract Aspergillus fumigatus (A.f.) the cause of invasive Aspergillosis (IA) has become a leading death in immunosuppressed populations, due to lack effective therapies or vaccines. Studies using anti-A.f. monoclonal antibodies indicate that they can protect against IA. However, detailed information on antigenic targets protective hampered development an vaccine. It is known sialylated epitopes found variety pathogens including fungi and Group B Streptococci (GBS) are involved attachment...
Abstract Aspergillus fumigatus (A.f.) the cause of invasive Aspergillosis (IA) has become a leading death in immunosuppressed populations, due to lack effective therapies or vaccines. Studies using anti-A.f. monoclonal antibodies indicate that they can protect against IA. However, detailed information on antigenic targets protective hampered development an vaccine. Sialylated epitopes found variety pathogens including fungi and Group B Streptococci (GBS) are thought be major virulence...
Abstract SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of respiratory disease COVID-19. Detecting antibodies against virus is an essential tool for tracking infections developing vaccines. Such tests, primarily utilizing enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative value representing quantity specific antibodies). Quantitation vital determining stability decline...