A5006778688- Vibrio bacteria research studies
- Advanced MRI Techniques and Applications
- Aquaculture disease management and microbiota
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Ubiquitin and proteasome pathways
- Trace Elements in Health
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Microtubule and mitosis dynamics
- Medical Imaging Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- COVID-19 Clinical Research Studies
- Lipoproteins and Cardiovascular Health
- Advanced Proteomics Techniques and Applications
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Clinical Laboratory Practices and Quality Control
- Neurological disorders and treatments
- Antibiotic Resistance in Bacteria
- Mass Spectrometry Techniques and Applications
- Disaster Response and Management
- Mitochondrial Function and Pathology
- Enterobacteriaceae and Cronobacter Research
- interferon and immune responses
Emory University
2019-2024
Centers for Disease Control and Prevention
2021-2023
Ministère de la Santé
2023
Center for Surveillance, Epidemiology, and Laboratory Services
2023
CDC Foundation
2022
National Center for Emerging and Zoonotic Infectious Diseases
2022
University of Chicago
2011-2019
Johns Hopkins University
2016-2018
Royal Adelaide Hospital
2002-2008
University of Wisconsin–Madison
2007
Late endosome-resident interferon-induced transmembrane protein 3 (IFITM3) inhibits fusion of diverse viruses, including Influenza A virus (IAV), by a poorly understood mechanism. Despite the broad antiviral activity IFITM3, viruses like Lassa (LASV), are fully resistant to its inhibitory effects. It is currently unclear whether resistance arises from highly efficient machinery that capable overcoming IFITM3 restriction or ability enter cellular sites devoid this factor. Here, we constructed...
Genomic DNA was extracted and sequenced on MiSeq instruments (Illumina) following Nextera XT or Prep
On September 30, 2022, after >3 years with no confirmed cholera cases (1), the Directorate of Epidemiology, Laboratories and Research (DELR) Haitian Ministry Public Health Population (Ministère de la Santé Publique et [MSPP]) was notified two patients acute, watery diarrhea in metropolitan area Port-au-Prince. Within 2 days, Haiti's National Laboratory bacterium Vibrio cholerae O1 specimens from suspected infection, an outbreak investigation began immediately. As January 3, 2023, >20,000 had...
Functional magnetic resonance imaging (fMRI) of the brain is often degraded by bulk head motion. Algorithms that address this retrospective re-registration images in an fMRI time series are all fundamentally limited any motion occurs through-plane. Here, a technique described can account for such prospective correction real time. A navigator echo used before every image acquisition to detect superior/inferior displacements head. The displacement information then adjust plane excitation...
There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all these diseases, mutant protein aggregates into neuronal inclusions; it generally, although not universally, believed aggregation underlying cause observed degeneration. effort to examine role in facilitating aggregation, investigators have used synthetic peptides as model systems. Analysis kinetic data led...
Abstract Since February 2022, Malawi has experienced a cholera outbreak of >54,000 cases. We investigated 6 cases in South Africa and found that isolates linked to the were Vibrio cholerae O1 serotype Ogawa from seventh pandemic El Tor sublineage AFR15, indicating new introduction into south Asia.
Abstract Global head motion occurring between successive image acquisitions during a functional MRI time series can corrupt the signal of physiologic brain activation, potentially invalidating interpretation final activation map from that particular fMRI series. By approximating as rigid body, multiaxial global be decomposed into orthogonal linear and rotational components. This paper describes method using orbital navigator echoes to provide prospective correction for both through–plane...
The Anaphase Promoting Complex/Cyclosome (APC/C) is a ubiquitin E3 ligase that functions as the gatekeeper to mitotic exit. APC/C activity controlled by an interplay of multiple pathways during mitosis, including spindle assembly checkpoint (SAC), are not yet fully understood. Here, we show sumoylation APC4 subunit peaks mitosis and critical for timely activation anaphase onset. We have also identified functionally important SUMO interacting motif in cullin-homology domain APC2 located near...
Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks occurred at universities during Fall 2020, but little is known about risk factors for campus-associated infections or immunity provided by anti-SARS-CoV-2 antibodies in young adults.
Objective Characterize college student COVID-19 behaviors and attitudes during the early pandemic. Participants: Students on two university campuses in Wisconsin. Methods Surveys administered September November 2020. Results Few students (3–19%) participated most in-person activities semester, with eating at restaurants as exception (72–80%) attending work (35%) parties (33%) also reported more frequently. The majority wore masks public (94–99%), but comparatively fewer (42%) did so parties....
A number of bacteria with close resemblance to Vibrio cholerae have been isolated over the years by Centres for Disease Control and Prevention (CDC), which could not be assigned a proper taxonomic designation on basis results from preliminary identification methods. Nine such isolates found share 16S rRNA gene identity exceeding 99 % V. cholerae, yet DNA–DNA hybridization (60.4–62.1 %) average nucleotide values (94.4–95.1 were below species cut-off, indicating potentially novel species....
Abstract Background The extent to which vaccinated persons who become infected with SARS-CoV-2 contribute transmission is unclear. During a Delta variant outbreak among incarcerated high vaccination rates in federal prison, we assessed markers of viral shedding and unvaccinated persons. Methods Consenting confirmed infection provided mid-turbinate nasal specimens daily for 10 consecutive days reported symptom data via questionnaire. Real-time reverse transcription-polymerase chain reaction...
Abstract Objectives Vibrio cholerae remains a significant public health threat in Africa, with antimicrobial resistance (AMR) complicating treatment. This study leverages whole-genome sequencing (WGS) of V. isolates from Côte d’Ivoire, Ghana, Zambia and South Africa to assess genomic diversity, AMR profiles, virulence, demonstrating the utility WGS for enhanced surveillance within PulseNet network. Methods We analysed clinical environmental sources (2010–2024) using Oxford Nanopore hybracter...
Objective: To assess the level of preparedness Australian hospitals, as perceived by senior emergency department physicians, for chemical, biological and radiological (CBR) incidents, well resources training available to their departments. Methods: Detailed questionnaires were mailed directors 86 hospital departments (EDs) in Australia accredited Australasian College Emergency Medicine. Questions covered planning, training, preparedness. Results: Responses received from 76 (88%): 73 reported...
Abstract Background We compared the rates of intraoperative parathyroid hormone (PTH) decline using Siemens Immulite® Turbo PTH and Roche Elecsys® short turnaround time assays in 95 consecutive surgical patients to investigate analytical (TAT) differences between tests performed operating room (OR) vs central clinical chemistry laboratory (CCL). Methods Serial blood samples from undergoing parathyroidectomy were collected measured 2 immunoassays. Specimens first 15 simultaneously OR CCL used...
Microcystins are toxic chemicals generated by certain freshwater cyanobacteria. These can accumulate to dangerous levels during harmful algal blooms. When exposed microcystins, humans at risk of hepatic injury, including liver failure. Here, we describe a method detect microcystins in human plasma using immunocapture followed protein phosphatase inhibition assay. At least 279 have been identified, and most these compounds share common amino acid, the Adda side chain. We targeted this chain...
To investigate the mechanisms underlining impaired intestinal absorption of lipids in zinc deficiency, apo-B content and chemical composition chylomicrons from marginally zinc-deficient rats fed 2.8 ppm dietary (ZD) were compared with those pair-fed (PF) ad libitum control (CT) groups an adequate level (30.8 ppm) zinc. Chylomicrons, obtained by cannulating mesenteric lymph, isolated ultracentrifugation at 1.3 X 10(6) g/min 12 C purified 2% agarose column chromatography. Apolipoprotein- (apo)...