A5040983615- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Bacterial Identification and Susceptibility Testing
- Nail Diseases and Treatments
- Education, sociology, and vocational training
- French Urban and Social Studies
- Psychoanalysis and Psychopathology Research
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Linguistics and Discourse Analysis
- Mycotoxins in Agriculture and Food
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Infections and bacterial resistance
- Social Sciences and Governance
- Transplantation: Methods and Outcomes
- Diphtheria, Corynebacterium, and Tetanus
- Nosocomial Infections in ICU
- Medical Malpractice and Liability Issues
- Streptococcal Infections and Treatments
- Long-Term Effects of COVID-19
- Health, Medicine and Society
Hôpital Bichat-Claude-Bernard
2015-2025
Assistance Publique – Hôpitaux de Paris
2016-2025
Université Paris Cité
2008-2024
Université Claude Bernard Lyon 1
2013-2024
Mère et Enfant en Milieu Tropical
2024
DYNAMYC MYCOLOGY
2024
Dynamique des Génomes et Adaptation Microbienne
2024
Institut de Recherche pour le Développement
2024
Sorbonne Université
2008-2023
Pitié-Salpêtrière Hospital
2015-2023
In recent years, we have moved from the sporadic description of terbinafine-resistant (TerR) Trichophyton spp. isolates to Indian outbreak due T. indotineae. Population flows spread TerR worldwide, altering local epidemiology. We conducted a prospective multicentric study determine relative frequency in France (Paris area) and newly introduced indotineae species. were screened by terbinafine-containing-agar-medium (TCAM) method confirmed EUCAST. Sequencing methods used identify...
Laboratory diagnosis of histoplasmosis is based on various methods, including microscopy, culture, antigen, and DNA detection Histoplasma capsulatum var. or duboisii. To improve sensitivity existing real-time quantitative PCR (qPCR) assays, we developed a new RT-qPCR assay that allows amplification whole nucleic acids spp. validated suspected cases. The limit was 20 copies, the specificity against 114 fungal isolates/species restricted to Whole 1319 prospectively collected consecutive...
Trichophyton indotineae is an emerging pathogen which recently spread from India to Europe and that more prone than other species of the mentagrophytes complex show resistance terbinafine, resulting in necessity rapid identification. Here, we improved online MSI-2 MALDI-TOF identification tool order identify T. indotineae. By multiplying culture conditions (2 media 6 stages growth) prior protein extractions for both test isolates reference strains, added 142 references corresponding 12...
We report the first identification of a fluconazole-resistant Candida parapsilosis (FR- Cp ) strain in our hospital, which subsequently caused an outbreak involving 17 patients (12 deaths) within 26-bed French intensive care unit. Microsatellite genotyping confirmed that all FR- isolates belonged to same clone.
The taxonomy of Aspergillus species has recently been revolutionized with the introduction cryptic and section concepts. However, their species-level identification in routine laboratories remains a challenge. aim this study was to prospectively assess accuracy various using mass spectrometry (MSI) platform, an independent freely accessible online database. Over 12-month period, when select set MSI users identified species, they were contacted requested send isolates our laboratory for...
The aim of this study was to evaluate diagnostic means, host factors, delay occurrence, and outcome patients with COVID-19 pneumonia fungal coinfections in the intensive care unit (ICU). From 1 February 31 May 2020, we anonymously recorded COVID-19-associated pulmonary aspergillosis (CAPA), fungemia (CA-fungemia), pneumocystosis (CA-PCP) from 36 centers, including results on biomarkers respiratory specimens serum. We collected data 154 episodes CAPA, 81 CA-fungemia, 17 CA-PCP, 5 other mold...
Bacterial identification and antibiotic susceptibility testing currently require 48 h when a first blood culture (BC) is positive for clustered gram-positive cocci on direct smear examination (DSE). Meanwhile, treatment often inadequate, reducing the chances of effective or creating unnecessary selective pressure. A new real-time PCR (RT-PCR) technique that differentiates Staphylococcus aureus from coagulase-negative staphylococci (CoNS) detects methicillin resistance in 90 min BC bottles...
Aspergillus spp. of section Usti (A. ustus) represent a rare cause invasive aspergillosis (IA). This multicenter study describes the epidemiology and outcome A. ustus infections.Patients with isolated from any clinical specimen were retrospectively identified in 22 hospitals 8 countries. When available, isolates sent for species identification (BenA/CaM sequencing) antifungal susceptibility testing. Additional cases by review literature. Cases classified as proven/probable IA or no...
The antifungal susceptibility of
Aspergillus cryptic species are increasingly recognised causes of diseases, including life-threatening invasive aspergillosis (IA). However, as their accurate identification remains challenging in a routine practice, few is known from clinical and epidemiological perspective. Recently, the MSI application has emerged powerful tool for detection species. We aimed to use network users conduct multicentre prospective screening species-related IA analyse epidemiological, mycological...
Abstract Background While invasive fusariosis and lomentosporiosis are known to be associated with fungemia, overall data on mold-related fungemia limited, hampering early management. This study aimed describe the epidemiology of mold-positive blood cultures. Methods Epidemiological clinical cultures from 2012 2022 were obtained RESSIF database. Pseudofungemia was excluded using modified Duthie Denning criteria. Univariable multivariable Firth logistical regression used factors 90-day...
Abstract Aspergillus fumigatus is associated with various invasive, chronic and allergic fungal diseases. The emergence of environmental azole-resistant strains complicates the treatment these infections. use whole-genome sequencing (WGS), which widely used to study bacterial viral outbreaks, could be beneficial for characterizing A. outbreaks. Here, nine cyp51A TR34/L98H isolated during an outbreak in a French hospital between November 2021 October 2022 were studied. genetic relatedness was...
Invasive fungal infections (IFI) are complications after liver transplantation involving high morbidity and mortality. (1,3)-β-d-glucan (BG) is a biomarker for IFI, but its utility remains uncertain. This study was designed to evaluate the impact of BG following their diagnosis. Between January 2013 May 2016, 271 transplants were performed in our institution. Serum samples tested (Fungitell®, Associates Cape Code Inc., Falmouth, MA, USA) at least weekly between discharge patients. Nineteen...
Background Influenza-vaccination rates among healthcare workers (HCW) remain low worldwide, even during the 2009 A(H1N1) pandemic. In France, this vaccination is free but administered on a voluntary basis. We investigated factors influencing HCW influenza vaccination. Methods June–July 2010, from wards of five French hospitals completed cross-sectional survey. A multifaceted campaign aimed at improving coverage in hospital group was conducted before and Using an anonymous self-administered...
ABSTRACT Aspergillus section Terrei is a species complex currently comprised of 14 cryptic whose prevalence in clinical samples as well antifungal susceptibility are poorly known. The aims this study were to investigate A. isolates at the level and perform analyses by reference commercial methods. Eighty-two collected from 8 French university hospitals. Molecular identification was performed sequencing parts beta-tubulin calmodulin genes. MICs or minimum effective concentrations (MECs)...
Fusarium spp. are widespread environmental fungi as well pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order allow a fast, accurate free-access identification by matrix-assisted laser desorption ionization—time flight (MALDI-TOF) mass spectrometry. Taking advantage MSI user network, we aim study species...
We determined the susceptibility of 182 Fusarium species isolates to five antifungal drugs (amphotericin B, voriconazole, posaconazole, isavuconazole, and terbinafine) by EUCAST method. Based on latest taxonomic insights, collected from 20 European centers were distributed into seven complexes 27 species.