A5005617827- Cellular Mechanics and Interactions
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Chromosomal and Genetic Variations
- melanin and skin pigmentation
- Cellular transport and secretion
- Caveolin-1 and cellular processes
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Protease and Inhibitor Mechanisms
- CRISPR and Genetic Engineering
- Hippo pathway signaling and YAP/TAZ
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Calcium signaling and nucleotide metabolism
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Ion Channels and Receptors
- Cold Atom Physics and Bose-Einstein Condensates
- Nuclear Structure and Function
Institut Curie
2020-2025
Centre National de la Recherche Scientifique
2020-2025
Université Paris Sciences et Lettres
2020-2025
Sorbonne Université
2025
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2021
Institut National des Sciences Appliquées Rouen Normandie
2012
Hungarian Academy of Sciences
2011
TU Wien
2011
HUN-REN Institute for Nuclear Research
2011
Diploid and stable karyotypes are associated with health fitness in animals. By contrast, whole-genome duplications-doublings of the entire complement chromosomes-are linked to genetic instability frequently found human cancers1-3. It has been established that duplications fuel chromosome through abnormal mitosis4-8; however, immediate consequences tetraploidy first interphase not known. This is a key question because single duplication events such as cytokinesis failure can promote...
Errors during cell division lead to aneuploidy, which is associated with genomic instability and transformation. In response cells activate the tumour suppressor p53 elicit a surveillance mechanism that halts proliferation promotes senescence. The molecular sensors trigger this checkpoint are unclear. Here, using tunable system of chromosome mis-segregation, we show mitotic errors nuclear deformation, softening, lamin heterochromatin alterations, leading rapid p53/p21 activation upon exit in...
Abstract The human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial cells. These cells are responsible for production of a diversity cell types, but successive fate decisions taken by individual progenitors remain unknown. Here we developed semi-automated live/fixed correlative imaging method map division modes in early fetal tissue and cerebral organoids. Through live analysis hundreds dividing progenitors, show...
Extracellular vesicles (EVs) facilitate the transfer of proteins, lipids, and genetic material between cells are recognized as an additional mechanism for sustaining intercellular communication. In epidermis, communication melanocytes keratinocytes is tightly regulated to warrant skin pigmentation. Melanocytes synthesize melanin pigment in melanosomes that transported along dendrites prior keratinocytes. EVs secreted by modulate pigmentation [(A. Lo Cicero et al. , Nat. Commun. 6 7506...
Invadosomes and caveolae are mechanosensitive structures that implicated in metastasis. Here, we describe a unique juxtaposition of caveola clusters matrix degradative invadosomes at contact sites between the plasma membrane cancer cells constricting fibrils both 2D 3D type I collagen environments. Preferential association straight segments fibrils, bent was observed along with remodelling. Caveola recruitment precedes is required for invadosome formation activity. Reciprocally, disruption...
Abstract Tissue homeostasis requires regulation of cell–cell communication, which relies on signaling molecules and cell contacts. In skin epidermis, keratinocytes secrete factors transduced by melanocytes into cues promoting their pigmentation dendrite outgrowth, while transfer melanin pigments to convey photoprotection. How epidermal cells integrate these functions remains poorly characterized. Here, we show that caveolae are asymmetrically distributed in particularly abundant at the...
Migrating cells present a variety of paths, from random to highly directional ones. While movement can be explained by basal intrinsic activity, persistent requires stable polarization. Here, we quantitatively address emergence migration in (hTERT)-immortalizedRPE1 (retinal pigment epithelial) over long timescales. By live cell imaging and dynamic micropatterning, demonstrate that the Nucleus-Golgi axis aligns with direction leading efficient movement. We show polarized trafficking is...
Tumor cells exposed to a physiological matrix of type I collagen fibers form elongated collagenolytic invadopodia, which differ from dotty-like invadopodia forming on the gelatin substratum model. The related scaffold proteins, TKS5 and TKS4, are key components mechanism assembly. molecular events through TKS proteins direct formation poorly defined. Using coimmunoprecipitation experiments, identification bound by mass spectrometry, in vitro pull-down we found an interaction between FGD1,...
Intracellular trafficking is mediated by transport carriers that originate membrane remodeling from donor organelles. Tubular contribute to the flux of lipids and proteins acceptor organelles, but how impose a tubular geometry on incompletely understood. Using imaging approaches cells in vitro systems, we show phosphatidylinositol-4-phosphate (PI4P) biogenesis lysosome-related organelles complex 1 (BLOC-1) govern formation, stability, functions recycling endosomal tubules. In vitro, BLOC-1...
Abstract The strong size increase of the human neocortex is supported both by amplification and basal translocation a neural stem cell population, radial glial cells (or bRG cells). Using live imaging second trimester fetal tissue cortical organoids, we identify two independent modes for colonization neocortex. On top an actomyosin-dependent movement called mitotic somal (MST), microtubule-dependent motion occurring during interphase, that call interphasic (IST). We show IST driven LINC...
Melanin pigments block genotoxic agents by positioning on the sun-exposed side of human skin keratinocytes' nucleus. How this position is regulated and its role in genome photoprotection remains unknown. By developing a model keratinocytes internalizing extracellular melanin into pigment organelles, we show that keratin 5/14 intermediate filaments mechanically control 3D perinuclear pigments, shielding DNA from photodamage. Imaging microrheology disease-related identify structural cages...
Unscheduled whole genome duplication (WGD), also described as unscheduled or non-physiological polyploidy, can lead to genetic instability and is commonly observed in human cancers. WGD generates DNA damage due scaling defects between replication factors content. As a result, repair mechanisms are thought be critical for ensuring cell viability proliferation under these conditions. In this study, we explored the role of homologous recombination Holliday junction resolution polyploidy vivo....
Abstract Under conditions of starvation, normal and tumor epithelial cells can rewire their metabolism toward the consumption extracellular proteins, including matrix‐derived components as nutrient sources. The mechanism pericellular matrix degradation by starved has been largely overlooked. Here it is shown that breast pancreatic patient‐derived xenograft explants increases one order magnitude upon amino acid growth factor deprivation. In addition, found collagenolysis requires invadopodia...
Abstract The human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial (bRG) cells. These cells are responsible for production of a diversity cell types, but successive fate decisions taken by individual progenitors remains unknown. Here, we developed semi-automated live/fixed correlative imaging method generate map bRG division modes in early fetal tissue and cerebral organoids. Through analysis over 1,000 dividing...
The phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway is frequently hyperactivated in triple-negative breast cancers (TNBCs) associated with poor prognosis and a therapeutic cancer management. Here, we describe the effects repression mTOR-containing complex 1 (mTORC1) through knockdown several key mTORC1 components or mTOR inhibitors used therapy. results an ∼10-fold increase extracellular matrix proteolytic degradation. Repression TNBC models, including...
The mechanistic target of rapamycin complex 1 (mTORC1) is part the amino acid sensing machinery that becomes activated on endolysosomal surface in response to nutrient cues. Branched actin generated by WASH and Arp2/3 complexes defines microdomains. Here, we find mTORC1 components close proximity We investigated for interactors lysosomal tether, RAGC, proteomics identified multiple filament capping proteins their modulators. Perturbation RAGC function affected size actin, consistent with a...
Abstract Under conditions of starvation, normal and tumor epithelial cells can rewire their metabolism towards the consumption extracellular matrix-derived components as nutrient sources. The mechanism pericellular matrix degradation by starved has been largely overlooked. Here we show that breast pancreatic patient-derived xenograft explants increases one order magnitude upon amino acid growth factor deprivation. In addition, found collagenolysis requires invadopodia components, TKS5...
We present experimental studies of the transmission slow 4.5 keV Ar9+ ions through a single cylindrical‐shaped glass capillary macroscopic dimension with large aspect ratio. find stable micrometer‐scale beam considerable intensity after charge‐up phase, in which self‐organized process leads to formation guiding electric field. show time evolution transmitted sample for various tilt angles.