A5100674349- Microbial Metabolic Engineering and Bioproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Algal biology and biofuel production
- Protein purification and stability
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Nanofabrication and Lithography Techniques
- Advanced Control Systems Optimization
- Microbial Metabolites in Food Biotechnology
- Phytase and its Applications
- Cerebrovascular and genetic disorders
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Birth, Development, and Health
- Immunotherapy and Immune Responses
Wenzhou Medical University
2024
Johns Hopkins University
2018-2023
Anhui Medical University
2023
Affiliated Hospital of Zunyi Medical College
2019
University of Toronto
2002-2018
Hohai University
2017
University of California, Berkeley
2016
China Agricultural University
2011-2015
Hefei University of Technology
2014
Anhui University
2014
A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene pairs for synthetic interactions, generating quantitative profiles approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. network based on reveals a functional cell which similar biological processes cluster together coherent subsets, and highly correlated delineate specific pathways to define gene function. The global identifies cross-connections between bioprocesses, mapping...
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and 350,000 positive interactions. This comprehensive maps interactions essential gene pairs, highlighting genes as densely connected hubs. Genetic profiles enabled assembly of hierarchical model cell function, including modules corresponding to protein complexes pathways, biological processes, cellular compartments. Negative...
Trigenic interactions in yeast link bioprocesses To dissect the genotype-phenotype landscape of a cell, it is necessary to understand between genes. Building on digenic protein-protein interaction network, Kuzmin et al. created trigenic by using synthetic genetic array (see Perspective Walhout). Triple-mutant analyses indicated that majority genes with associations functioned within same biological processes. These converged networks identified landscape. Although overall effects were weaker...
Background Preventing germline stem cell proliferation extends lifespan in nematodes and flies. So far, studies on germline-longevity signaling have focused daf-16/FOXO daf-12/VDR. Here, we report NHR-80/HNF4, a nuclear receptor that specifically mediates longevity induced by depletion of the germ line through mechanism implicates fatty acid monodesaturation. Methods Findings nhr-80/HNF4 is animals lacking required for their extended longevity. Overexpressing increases germline-less animals....
Abstract We present a method for high-resolution genetic mapping that takes advantage of the ordered set viable gene deletion mutants, which form colinear markers covering almost every centimorgan Saccharomyces cerevisiae genome, and synthetic array (SGA) system, automates construction double mutants formed by mating meiotic recombination. The Cbk1 kinase signaling pathway, consists minimally CBK1, MOB2, KIC1, HYM1, TAO3 (PAG1), controls polarized morphogenesis activation Ace2 transcription...
Constraint-based modeling has been applied to analyze metabolism of numerous organisms via flux balance analysis and genome-scale metabolic models, including mammalian cells such as the Chinese hamster ovary (CHO) cells-the principal cell factory platform for therapeutic protein production. Unfortunately, application model methodologies using conventional biomass objective function is challenged by presence overly-restrictive constraints, essential amino acid exchange fluxes that can lead...
Methyltransferase-like protein 7A (METTL7A), an RNA N6-methyladenosine (m6A) methyltransferase, has attracted much attention as it been found to be closely associated with various types of tumorigenesis and progression. This study provides a comprehensive assessment METTL7A from pan-cancer perspective using multi-omics data. The gene ontology enrichment analysis METTL7A-binding proteins revealed close association methylation lipid metabolism. We then explored the expression in normal...
Abstract Nutrient availability is critical for growth of algae and other microbes used generating valuable biochemical products. Determining the optimal levels nutrient supplies to cultures can eliminate feeding excess nutrients, lowering production costs reducing pollution into environment. With advent omics bioinformatics methods, it now possible construct genome-scale models that accurately describe metabolism microorganisms. In this study, a model green alga Chlorella vulgaris ( i CZ946)...
Necroptosis and endoplasmic reticulum (ER) stress has been implicated in acute chronic liver injury. Activated eukaryotic initiation factor 2 alpha (eIF2α) attenuates protein synthesis relieves the load of folding ER. In this study, we aimed to analyze impact eIF2α phosphorylation on hepatocyte necroptosis injury.Male BALB/c mice were injected with tunicamycin or d-galactosamine, LO2 cells incubated induce 4-Phenylbutyric acid (PBA) salubrinal used inhibit ER dephosphorylation, respectively....
Sodium butyrate (NaBu) is not only well-known for enhancing protein production, but also degrades glycan quality. In this study, supplied by the precursor molecule 1,3,4-O-Bu3 ManNAc applied to overcome negative effects of NaBu on quality while simultaneously increasing productivity model recombinant erythropoietin (EPO). The beneficial impact EPO quality, evident in wild-type CHO cells, particularly pronounced glycoengineered cells with stable overexpression β-1,4- and...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus utilizes the extensively glycosylated spike (S) protein protruding from viral envelope to bind angiotensin-converting enzyme-related carboxypeptidase (ACE2) as its primary receptor mediate host-cell entry. Currently, main recombinant S production hosts are Chinese hamster ovary (CHO) and human embryonic kidney (HEK) cells. In this study, a truncated at transmembrane domain engineered express C-terminal trimerization motif...
Abstract Algal cultivations are strongly influenced by light and dark cycles. In this study, genome-scale metabolic models were applied to optimize nutrient supply during alternating cycles of Chlorella vulgaris . This approach lowered the glucose requirement 75% nitrate 23%, respectively, while maintaining high final biomass densities that more than 80% glucose-fed heterotrophic culture. Furthermore, strictly controlling feeding based on model-input, yields biomass, lutein, fatty acids per...
Abstract
In order to improve some characteristics of a β‐1,3‐1,4‐glucanase from Bacillus subtilis MA139, directed evolution was conducted in this study. After error‐prone PCR, the gene, glu‐opt , cloned into vector pBGP1 and transformed Pichia pastoris X‐33 construct mutant library. Three variants named as 7‐32, 7‐87, 7‐115 were screened 8000 colonies. Amino‐acid sequence analysis showed that these mutants had one or two amino‐acid substitutions (7‐32: T113S, 7‐87: M44V/N53H, 7‐115: N157D). The...
In recent years, α-galactosidase has been attracting more and attention because of its potential applications in many aspects. Using reverse transcription-polymerase chain reaction rapid amplification cDNA ends, a full-length sequence composed 2,439 bp was cloned from Penicillium janczewskii zaleski subcloned into pPICZαA transformed Pichia pastoris strain X-33. 10-L fermentor, the recombinant yeast expressed with yield 254 U/mL by methanol induction for 120 h. The enzyme showed optimal...
Abstract Mammalian cell culture processes rely heavily on empirical knowledge in which process control remains a challenge due to the limited characterization/understanding of metabolism and inability predict behaviors. This study facilitates Chinese hamster ovary (CHO) through forecast‐based feeding approach that predicts multiple essential amino acids levels from easily acquired viable density data. Multiple growth behavior forecast extrapolation approaches are considered with logistic...