A5064916866- Chromatin Remodeling and Cancer
- Hedgehog Signaling Pathway Studies
- Neuroblastoma Research and Treatments
- Kruppel-like factors research
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Genetic Syndromes and Imprinting
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Retinoids in leukemia and cellular processes
- Sarcoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Caveolin-1 and cellular processes
- Escherichia coli research studies
- Heat shock proteins research
Shanghai Jiao Tong University
2013-2025
Dongguan People’s Hospital
2025
Yunnan Agricultural University
2025
State Key Laboratory of Oncogene and Related Genes
2022-2024
Shanghai Children's Hospital
2022
Emory University
2008-2011
Shandong University
2003-2004
Chinese Academy of Sciences
2004
Scripps Research Institute
2004
Shanghai Institutes for Biological Sciences
2004
Abstract Osteosarcoma (OS) is a primary malignant bone tumor that most commonly affects children, adolescents, and young adults. Here, we comprehensively analyze genomic, epigenomic transcriptomic data from 121 OS patients. Somatic mutations are diverse within the cohort, only TP53 significantly mutated. Through unsupervised integrative clustering of multi-omics data, classify into four subtypes with distinct molecular features clinical prognosis: (1) Immune activated (S-IA), (2) suppressed...
Chemoresistance contributes to cancer relapse and increased mortality in a variety of types, raising pressing need better understand the underlying mechanism. MUC1 is abnormally overexpressed numerous carcinomas associated with poor prognosis. However, functional significance chemoresistance has not been fully elucidated. Here, we showed that expression was considerably induced cells had acquired at both transcriptional post-translational levels. Using gain- loss-of function approaches,...
Abstract Long noncoding RNA KCNQ1OT1 participates in the regulation of imprinted genes within kcnq1 domain. But its roles carcinogenesis and metastasis remain largely elusive. Herein, we evaluated potential non‐small‐cell lung cancer (NSCLC) progression. We demonstrated that level was upregulated NSCLC tissues cell lines. High correlated with poor overall progression‐free survival patients. facilitated proliferation, migration, invasion H460 cells. Furthermore, knockdown reduced expression...
During epithelial homeostasis, stem cells divide to produce progenitor cells, which not only proliferate generate the cell mass but also respond cellular signaling transition from a proliferative state differentiation state. Such involves functional alterations of transcriptional factors, yet underlying molecular mechanisms are poorly understood. Recent studies have implicated Kruppel-like factors (KLFs) including KLF5 in renewal and maintenance stem/progenitor cells. Here we demonstrate...
KLF5 plays important roles in a variety of cellular processes including proliferation and differentiation. Recently was shown to reverse its function proliferative p15 regulation upon transforming growth factor-β (TGFβ)-mediated acetylation. To understand how acetylation functions TGFβ-induced transcription, we characterized the interactions with other transcription factors promoter DNA elements context TGFβ. interacted Smad2–4 Miz-1 TGFβ-independent manner, but Myc only when TGFβ activated,...
Suppressors of cytokine signaling, SOCS1 and SOCS3, are important negative regulators Janus kinase 2/signal transducers activators transcription which is constitutively activated in myeloproliferative neoplasms (MPNs) leukemia. Curcumin has been shown to possess anticancer activity through different mechanisms. However, whether curcumin can regulate the expression SOCS3 still unknown. Here, we found that elevated via triggering acetylation histone regions promoter K562 HEL cells. As a novel...
Hypoxia-inducible factor-1 (HIF-1), consisting of oxygen-sensitive HIF-1alpha and constitutively expressed HIF-1beta subunits, is a master transcriptional activator for cellular response to hypoxia. To explore direct HIF-1 targets, here we used differential gel electrophoresis (DIGE) compare the HIF-1-regulated proteins between leukemic U937T-cell line with without conditional induction protein by tetracycline-off system. Among upregulated identified, mRNA levels annexin A1,...
Abstract Cancer stem cells (CSCs) are often enriched after chemotherapy and contribute to tumor relapse. While epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) widely used for the treatment of diverse types cancer, whether EGFR-TKIs effective against chemoresistant CSCs in cervical cancer is largely unknown. Here, we reveal that EGFR correlates with reduced disease-free survival patients chemotherapy. Erlotinib, an EGFR-TKI, effectively impedes enrichment...
KLF5 (Krüppel-like factor 5) is a multifunctional transcription involved in cell proliferation, differentiation and carcinogenesis. In addition to frequent inactivation different types of human cancers, including breast cancer, has been identified as an essential co-factor for the TGF-β (transforming growth β) tumour suppressor. our previous study demonstrating negative regulation ER (oestrogen receptor α) function by cancer cells [Guo, Dong, Zhao, Sun, Li Dong (2010) Int. J. Cancer 126,...
Alzheimer's disease (AD) is a common neurodegenerative disorder with progressive cognitive impairment in the elderly. Beta-amyloid (Aβ) formation and its accumulation brain constitute one of pathological hallmarks AD. Until now, how to modulate Aβ hippocampal neurons remains big challenge. Herein, we investigated whether exosomal transfer microRNA (miR) relates amyloid pathology recipient neuron cells. We isolated circulating small extracellular vesicles (sEVs) from AD patients healthy...
Preeclampsia (PE) is a multisystem progressive disease that occurs during pregnancy. Previous studies have shown the immune system involved in placental trophoblast function and pathological process of uterine vascular remodeling PE. However, its molecular mechanism still unclear. This study aimed to identify critical genes cells The PE-related GSE74341 GSE160888 datasets were downloaded from Gene Expression Omnibus (GEO) database, differential expression analysis, weighted gene...
CRISPR/Cas9, as a well-established gene editing technology, has been applied in numerous model organisms, but its application wild-type E. coli remains limited. Pathogenic coli, major cause of foodborne illnesses and intestinal inflammation humans animals, poses significant global public health threat. The valine-glycine repeat protein G (VgrG) is key virulence factor that enhances pathogenicity. In this study, PCR was used to identify 50 strains carrying the VgrG2 out 83 wild pathogenic...
Background: Alzheimer’s disease (AD) is the most common neurodegenerative affecting elderly, with its diagnosis at early stages crucial for effective intervention. Recent evidence increasingly supports role of epigenetic alterations in AD pathogenesis, highlighting need innovative biomarkers that reflect these changes. This study aimed to characterize genome-wide DNA methylation profiles cell-free peripheral blood potential associated AD. Methods: The Illumina Infinium array was utilized...
Intervertebral disc degeneration (IDD) is a major contributor to low back pain, prevalent and debilitating condition. Nucleus pulposus (NP) cells are essential for maintaining homeostasis, their dysfunction plays crucial role in IDD development. This study aimed explore the potential of miR-1275, delivered via mesenchymal stem cell-derived extracellular vesicles (MSCs-EVs), pathogenesis elucidate underlying molecular mechanisms through vitro investigations. Decreased miR-1275 expression...
Abstract AML1‐ETO, a fusion protein generated by the chromosomal translocation t(8;21), is frequently associated with acute myeloid leukemia (AML). In addition to blocking differentiation, AML1‐ETO also shown induce growth arrest in AML cells, which unfavorable for leukemogenesis harboring t(8;21) translocation. However, its precise mechanism still unclear. Here we provide first demonstration that conditional expression of ecdysone‐inducible system dramatically increases connexin 43 (CX43),...
Kruppel-like factor 5 (KLF5) is implicated in human breast cancer by frequent genomic deletion and expressional deregulation, but the molecular mechanisms which KLF5 affects tumorigenesis are still unknown. This study was conducted to examine whether how function of estrogen receptor (ER) cells. Using different cell lines, we found that restored expression inhibited estrogen-promoted proliferation ER-positive MCF-7 T-47D lines had no effect on ER-negative SK-BR-3 Transcriptional activity ER...
Clear cell renal carcinoma (ccRCC) is the major subtype of (RCC) that resistant to conventional radiation and chemotherapy. It a challenge explore effective therapeutic targets drugs for this kind cancer. Transcription factor Krüppel-like 5 (KLF5) exerts diverse functions in various tumor types. By analyzing cohorts Cancer Genome Atlas (TCGA) data sets, we find KLF5 expression suppressed ccRCC patients higher level associated with better prognostic outcome. Our further investigations...
Aberrant activation of the Hedgehog (Hh) signaling pathway plays important roles in oncogenesis and therapeutic resistance several types cancer. The clinical application FDA-approved Hh-targeted smoothened inhibitors (SMOi) is hindered by emergence primary or acquired drug resistance. Epigenetic transcriptional-targeted therapies represent a promising direction for developing improved anti-Hh therapies. In this study, we integrated epigenetic/transcriptional-targeted small-molecule library...
Objectives: Neuroblastoma (NB), a pediatric malignancy of the peripheral nervous system, is characterized by epigenetic and transcriptional (EP-TF) anomalies. This study aimed to develop an EP-TF clinical prognostic model for NB using CRISPR-Cas9 knockout screening. Results: An integrative analysis was conducted screening in vitro vivo with public datasets identify 35 genes that exhibited highest expression were highly dependent on cancer viability. After univariate analysis, 27 these...