A5033775362- Hepatitis C virus research
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- HIV Research and Treatment
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- HIV/AIDS Research and Interventions
- COVID-19 Clinical Research Studies
- T-cell and Retrovirus Studies
- HIV/AIDS drug development and treatment
- Viral gastroenteritis research and epidemiology
- Systemic Lupus Erythematosus Research
- Virology and Viral Diseases
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Blood groups and transfusion
- Syphilis Diagnosis and Treatment
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- COVID-19 Impact on Reproduction
- Cytomegalovirus and herpesvirus research
- Vaccine Coverage and Hesitancy
National Center for Clinical Laboratories
2015-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2025
Academy of Medical Sciences
2020-2025
Beijing Hospital
2015-2025
Stanford Health Care
2023
Stanford Blood Center
2023
Ministry of Agriculture and Rural Affairs
2022
Chinese Academy of Fishery Sciences
2022
Academia Sinica
2020-2021
Peking Union Medical College Hospital
2013-2021
Abstract Because of high rates 2019 novel coronavirus disease in Wuhan, China, Wuhan Blood Center began screening for severe acute respiratory syndrome 2 RNA on January 25, 2020. We screened donations real-time and retrospectively found plasma samples positive viral from 4 asymptomatic donors.
Zoonotic diseases are infectious that naturally transmitted from vertebrate animals to humans and vice versa. They caused by all types of pathogenic agents, including bacteria, parasites, fungi, viruses prions. Although they have been recognised for many centuries, their impact on public health has increased in the last few decades due a combination success reducing spread human through vaccination effective therapies emergence novel zoonotic diseases. It is being increasingly One Health...
Recently, microRNA (miRNA)‐mediated RNA interference has been developed as a useful tool in gene function analysis and therapy. A major obstacle miRNA‐mediated RNAi is cellular delivery, which requires an efficient flexible delivery system. The self‐assembly of the MS2 bacteriophage capsids used to develop virus‐like particles (VLPs) for drug delivery. However, VLP‐mediated miRNA not yet reported. We therefore Escherichia coli expression system produce pre‐miR 146a contained VLPs, then...
Prostate cancer (PCa) is the most diagnosed in western male population with high mortality. Recently, alternative approaches based on immunotherapy including mRNA vaccines for PCa have shown therapeutic promise. However, vaccine, several disadvantages such as instability of mRNA, cost gold particles, limited production scale mRNA‐transfected dendritic cells vitro , limit their development. Herein, recombinant bacteriophage MS2 virus‐like particles (VLPs), which interaction a 19‐nucleotide...
Methods: VLPs containing miR-146a, and the control VLPs, were prepared using an Escherichia coli expression system then administered to lupus-prone mice over a 12-day period. We performed enzyme-linked immunosorbent assay evaluate anti-dsDNA antibody, autoantibody nuclear antigen (ANA), total IgG IgM levels in serum. The of miR-146a was analyzed by qRT-PCR. SLE-related cytokines as well some toll-like receptor signaling pathway molecules also measured. Results: Treatment with MS2-miR146a VLP...
// Le Chang 1, 2 , Guojing Wang Tingting Jia 3 Lei Zhang 4 Yulong Li Yanxi Han 1 Kuo Guigao Lin Rui Jinming Lunan National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China Graduate School, Peking Union Medical College, Chinese Academy Sciences, Department Laboratory, Chaoyang Capital University, University Fifth School Medicine, Correspondence to: Li, e-mail: jmli@nccl.org.cn Wang, lunan99@163.com Keywords: hepatocellular carcinoma, maternally expressed...
In this study, we investigate the seroprevalence of SARS-CoV-2 antibodies among blood donors in cities Wuhan, Shenzhen, and Shijiazhuang China. From January to April 2020, 38,144 healthy three were tested for total antibody against followed by pseudotype neutralization tests, IgG, IgM testing. Finally, a 398 confirmed positive. The age- sex-standardized 18-60 year-old adults (18-65 Shenzhen) was 2.66% (95% CI: 2.24%-3.07%) 0.033% 0.0029%-0.267%) 0.0028% 0.0001%-0.158%) Shijiazhuang,...
This study compared the immunogenicity of inactivated SARS-CoV-2 vaccines between people living with HIV (PLWH) and HIV-negative individuals. We recruited 120 PLWH 53 individuals aged 18-59 years who had received an vaccine in two Chinese cities April June 2021. Blood samples were tested for vaccines. The prevalence severity adverse events associated similar seropositivity neutralizing activity against authentic SARS-CoV-2, total amount antibody (total antibody) S-IgG 71.3%, 81.9%, 92.6%,...
Aim : To study the pathology of two cases human Hendra virus infection, one with no clinical encephalitis and relapsing encephalitis. Methods Autopsy tissues were investigated by light microscopy, immunohistochemistry in situ hybridization. Results In patient acute pulmonary syndrome but not encephalitis, vasculitis was found brain, lung, heart kidney. Occasionally, viral antigens demonstrated vascular walls multinucleated endothelial syncytia absent. there severe inflammation, necrosis type...
In recent years, due to the concern of high rate burnout and attrition teachers in their early career teacher resilience has become a vital theme study professional development. However, little been done China. This contextualized development Chinese universities interviewed seven resilient novice foreign language (FL) explore risk factors confronting them contexts strategies they have employed cope with these challenges. In-depth interview data informants' reflective journals were analyzed...
ABSTRACT The branched DNA (bDNA) assay is a reliable method for quantifying the RNA of human immunodeficiency virus type 1 (HIV-1). positive controls and standards this detection HIV-1 consist naked RNA, which susceptible to degradation by RNase. Armored good candidate an RNase-resistant control or standard. However, its use has been limited maximal length exogenous packaged into virus-like particles routine armored technology. In present study, we produced long (armored L-RNA)...
Previously, we developed a novel microRNA (miRNA) delivery system based on bacteriophage MS2 virus-like particles (MS2 VLPs). In this current study, used to transport miR-146a into human peripheral blood mononuclear cells (PBMCs), and demonstrated the inhibition of osteoclastogenesis in precursors. Two cytokines, receptor activator NF-κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) were induce osteoclastogenesis. VLPs transfected PBMCs. qRT-PCR was applied measure expression...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA could be detected in the blood of infected cases. From February 9, all establishments Hubei province, China, implemented nucleic acid testing (NAT) for SARS-CoV-2 among donors to ensure safety.Nucleic test screening individually (ID) or by minipool (MP) was performed according manufacturer's instructions. Inactivated culture supernatant SARS-CoV-2-infected Vero cells quantified droplet digital polymerase chain reaction (ddPCR)...
Abstract Background The HTLV-1 prevalence in China varies geographically, while HTLV-2 infection has rarely been found so far. Proviral load is one of the determining factors pathogenesis and progression related diseases. However, neither molecular assays nor commercial kits are available for diagnosis China. objective present study was to develop validate a TaqMan qPCR assay proviral quantification. Results A plasmid containing both interest fragment RNase P (RPPH1) gene constructed used...
Little is known about the diversity of residents' preferences for COVID-19 vaccines during time when management was downgraded in China. This study aims to investigate these using discrete choice experiment (DCE) and profile case best–worst scaling (BWS-2), assess concordance between two methods. Chinese residents recruited online survey were asked evaluate vaccine profiles through both DCE BWS-2 from April July 2023. Attributes included effectiveness, duration protection, risk severe...
The transmission of emerging and re-emerging arboviruses represent a critical challenge to blood transfusion safety worldwide. This study established comprehensive quality assurance system for Dengue virus (DENV), Zika (ZIKV), Chikungunya (CHIKV) nucleic acid testing (NAT) screening. included external assessment (EQA) implementation across all participating central stations performance evaluation six domestic screening reagents. Surveillance conducted in Yunnan Province Guangxi Zhuang...