A5078143491- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Cannabis and Cannabinoid Research
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Sleep and Wakefulness Research
- Parkinson's Disease Mechanisms and Treatments
- Tryptophan and brain disorders
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Memory and Neural Mechanisms
- Neurological Disease Mechanisms and Treatments
- Regulation of Appetite and Obesity
- Vaccine Coverage and Hesitancy
- Health Education and Validation
- Healthcare during COVID-19 Pandemic
- Cerebrovascular and Carotid Artery Diseases
- Neurological disorders and treatments
- Vagus Nerve Stimulation Research
- Science and Education Research
- Stress Responses and Cortisol
- Neurological Disorders and Treatments
- Metabolism and Genetic Disorders
University of Coimbra
2011-2023
Innsbruck Medical University
2018-2021
Universität Innsbruck
2018
Abstract Synaptic plasticity in the autoassociative network of recurrent connections among hippocampal CA3 pyramidal cells is thought to enable storage episodic memory. Impaired memory an early manifestation cognitive deficits Alzheimer’s disease (AD). In APP/PS1 mouse model AD amyloidosis, we show that associative long-term synaptic potentiation (LTP) abolished at stage. This caused by activation upregulated neuronal adenosine A 2A receptors (A R) rather than dysregulation NMDAR signalling...
Adenosine A2A receptors (A2AR) overfunction causes synaptic and memory dysfunction in early Alzheimer's disease (AD). In a β-amyloid (Aβ1-42)-based model of AD, we now unraveled that this involves an increased release ATP coupled to density activity ecto-5′-nucleotidase (CD73)-mediated formation adenosine selectively activating A2AR. Thus, CD73 inhibition with α,β-methylene-ADP impaired long-term potentiation (LTP) mouse hippocampal slices, which is occluded upon previous superfusion the...
Cannabinoid CB2 receptors (CB2Rs) are emerging as important therapeutic targets in brain disorders that typically involve neurometabolic alterations. We here addressed the possible role of CB2Rs regulation glucose uptake mouse brain. To aim, we have undertaken 1) measurement (3)H-deoxyglucose cultured cortical astrocytes and neurons acute hippocampal slices; 2) real-time visualization fluorescently labeled deoxyglucose superfused 3) vivo PET imaging cerebral (18)F-fluorodeoxyglucose uptake....
Abstract Adenosine is a neuromodulator mostly acting through A 1 (inhibitory) and 2A (excitatory) receptors in the brain. 2B (A 2 B R ) are G s/q ‐protein‐coupled with low expression As function largely unknown, we have now explored their role mouse hippocampus. We performed electrophysiological extracellular recordings hippocampal slices, immunological analysis of nerve terminals glutamate release slices synaptosomes. Additionally, ‐knockout ‐ KO C57/ BL 6 mice were submitted to behavioural...
Increased ATP release and its extracellular catabolism through CD73 (ecto-5′-nucleotidase) lead to the overactivation of adenosine A2A receptors (A2AR), which occurs in different brain disorders. A2AR blockade blunts mood memory dysfunction caused by repeated stress, but it is unknown if increased coupled CD73-mediated formation responsible for upon stress. This was now investigated adult rats subject stress 14 consecutive days. Frontocortical hippocampal synaptosomes from stressed displayed...
Ketone bodies can be used for cerebral energy generation in situ , when their availability is increased as during fasting or ingestion of a ketogenic diet. However, it not known how effectively ketone compete with glucose, lactate, and pyruvate the brain parenchyma. Hence, contributions exogenous 5.0 mM [1‐ 13 C]glucose 1.0 [2‐ C]lactate + 0.1 (combined C]pyruvate) to acetyl‐CoA production were measured both without [U‐ C]3‐hydroxybutyrate superfused rat hippocampal slices by C NMR...
Abstract Social interaction in an alternative context can be beneficial against drugs of abuse. Stress is known to a risk factor that exacerbate the effects addictive drugs. In this study, we investigated whether positive social are mediated through decrease stress levels. For purpose, rats were trained express cocaine or conditioned place preference (CPP). Behavioural, hormonal, and molecular markers evaluated. We found CPP decreased percentage incorrect transitions grooming corticosterone...
A crescente prevalência das doenças crônicas não transmissíveis (DCNTs) tem se tornado um desafio global significativo para a saúde pública. Estratégias de educação em emergem como uma abordagem fundamental na prevenção dessas condições, ao promover modificação comportamentos risco e adoção estilos vida saudáveis. Esta revisão integrativa teve objetivo avaliar o impacto estratégias educacionais DCNTs, considerando eficácia, duração os desfechos clínicos relacionados essas intervenções....
We recently showed that a single high dose of methamphetamine (METH) induces persistent frontal cortical monoamine depletion is accompanied by helpless-like behavior in mice. However, brain metabolic alterations underlying both neurochemical and mood remain unknown.Herein, we aimed at characterizing associated with early negative triggered METH. Adult C57BL/6 mice were injected METH (30 mg/kg, i.p.), their status was characterized after probing anxiety-related phenotypes 3 days...
Parkinson's disease (PD) prodromal stages comprise neuropsychiatric perturbations that critically compromise a patient's quality of life. These nonmotor symptoms (NMS) are associated with exacerbated innate immunity, hallmark overt PD. Physical exercise (PE) has the potential to improve deficits and modulate immune network including receptor for advanced glycation end products (RAGE) Toll-like receptors (TLRs) in distinct pathological settings. Accordingly, present study aimed test...
Corticosteroid and endocannabinoid actions converge on prefrontocortical circuits associated with neuropsychiatric illnesses. Corticosteroids can also modulate forebrain synapses by using effector systems. Here, we determined whether corticosteroids transmitter release directly in the frontal cortex and, doing so, they affect presynaptic CB1 cannabinoid receptor- (CB1R) mediated neuromodulation. By Western blotting of purified subcellular fractions rat cortex, found glucocorticoid receptors...
Evidence suggests that PKA activity in the nucleus accumbens (NAc) plays an essential role reward-related learning. In this study, we investigated whether is differentially involved expression of learning produced by either natural reinforcers or psychostimulants. For purpose, inhibited through a bilateral infusion Rp-cAMPS, specific inhibitor, directly into NAc. The effects inhibition NAc on concurrent conditioned place preference (CPP) for cocaine (drug) and social interaction (natural...
Alzheimer's disease (AD) is characterized by progressive memory deficits accompanied synaptic and metabolic deficits, namely of mitochondrial function. AD patients also display a disrupted circadian pattern. Thus, we now compared performance, plasticity, mitochondria function in 24-week-old non-transgenic (non-Tg) triple transgenic male mice modeling (3xTg-AD) at Zeitgeber 04 (ZT-4, inactive phase) ZT-16 (active phase). Using the Morris water maze test to minimize influence...
Satb2 is a DNA binding protein that specifically binds nuclear matrix attachment region and functions as regulator of the transcription large chromatin domains. Unlike its well addressed role during brain development, in adult under-investigated. It has been shown deletion from forebrain mice significantly impaired long term memory for contextual fear object recognition memory. The aim present study was to investigate effects appetitive stimuli such cocaine social interaction expression rat...