A5029928683- Neuroscience and Neuropharmacology Research
- Anesthesia and Neurotoxicity Research
- MicroRNA in disease regulation
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Circular RNAs in diseases
- Biological and pharmacological studies of plants
- Anesthesia and Sedative Agents
- Receptor Mechanisms and Signaling
- Cancer-related molecular mechanisms research
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Ginseng Biological Effects and Applications
- Cannabis and Cannabinoid Research
- Sleep and Wakefulness Research
- Neurotransmitter Receptor Influence on Behavior
- Berberine and alkaloids research
- Neuropeptides and Animal Physiology
- Tryptophan and brain disorders
- Genomics, phytochemicals, and oxidative stress
- Glutathione Transferases and Polymorphisms
- RNA modifications and cancer
- Ion channel regulation and function
- Extracellular vesicles in disease
Zunyi Medical University
2016-2025
Sichuan University
2011-2022
State Key Laboratory of Biotherapy
2011-2022
Metformin is a widely prescribed hypoglycemic drug. Many studies have shown its anti-cancer properties. In the present study, we aimed to explore effect of metformin on breast cancer and clarify underlying mechanism. Our results showed that induced ferroptosis in MDA-MB-231 cells through upregulating miR-324-3p expression. Overexpression inhibited cell viability. inhibitor promoted Further was mediated by directly targeting glutathione peroxidase 4 (GPX4). bound 3ʹ-UTR GPX4 led...
Background: The medial prefrontal cortex plays a crucial role in regulating consciousness. However, the specific functions of its excitatory and inhibitory networks during anesthesia remain uncertain. Here we explored hypothesis that somatostatin interneurons enhance effects sevoflurane by increasing GABA transmission to pyramidal neurons. Methods: EEG was utilized reflect depth anesthesia. Immunostaining fiber photometry were employed assess neuronal activities delivery. regulation activity...
General anesthesia has been used clinically for more than 170 years, yet its underlying mechanisms are still not fully understood. The parabrachial nucleus (PBN) in the brainstem known to be crucial regulating wakefulness and signs of arousal on cortical electroencephalogram (EEG). Lesions complex lead unresponsiveness a monotonous high-voltage, slow-wave EEG, which two main features general anesthesia. However, it is unclear whether how PBN functions process By recording levels calcium vivo...
Rheb1 is an immediate early gene that functions to activate mammalian target of rapamycin (mTor) selectively in complex 1 (mTORC1). We have demonstrated previously essential for myelination the CNS using a Nestin-Cre driver line deletes all neural cell lineages, and recent studies oligodendrocyte-specific CNP-Cre suggested preferential role mTORC1 spinal cord. Here, we examine Rheb1/mTORC1 mouse oligodendrocyte lineage separate Cre drivers progenitor cells (OPCs) including Olig1-Cre...
Cholinergic neurons in basal forebrain (BF) have long been considered to be the key regulation of cortical and behavioral arousal, cholinergic activation downstream region BF can arouse anesthetized rats. However, whether induce behavior EEG recovery from anesthesia is unclear. In this study, based on a transgenic mouse line expressing ChAT-IRES-Cre, we applied fiber photometry system combined with Gcamps expression found that both isoflurane propofol inhibit activity neurons, which closely...
Tumor-associated macrophages contribute to cell growth, development, and metastasis in various cancers. However, the underlying mechanisms of M2 macrophage that modulate progression gastric cancer (GC) remain largely unknown. In this study, we detected ratio GC tissues found proportion was increased tissues. We then co-cultured cells with M1 macrophages, respectively, assessed proliferation tumorigenicity by MTT colony formation assay. The results indicated promoted cells, but not. Besides,...
Background: Mechanisms of neuropathic pain are still largely unknown. Molecular changes in spinal dorsal horn may contribute to the initiation and development pain. Circular RNAs (circRNAs) have been identified as microRNA sponges involved various biological processes, but whether their expression profile condition is not reported. Methods: To test influences circRNA expression, we developed a sciatic chronic constriction injury (CCI) model rats. The CCI ipsilateral horns lumbar enlargement...
Electroencephalogram monitoring during propofol (PRO) anesthesia typically features low-frequency oscillations, which may be involved with thalamic reticular nucleus (TRN) modulation. TRN receives noradrenergic inputs from the locus coeruleus (LC). We hypothesized that specific connections in contribute to emergence PRO anesthesia. Intranuclei norepinephrine (NE) injections (n = 10) and designer receptors exclusively activated by drugs (DREADDs) were used investigate role of LC Whole-cell...
Abstract Aims The basal forebrain (BF) plays an essential role in wakefulness and cognition. Two subtypes of BF gamma‐aminobutyric acid (GABA) neurons, including somatostatin‐expressing (GABA SOM ) parvalbumin‐positive Parv function differently mediating the natural sleep–wake cycle. Since loss consciousness induced by general anesthesia cycle probably share similar mechanisms, it is important to clarify accurate roles these neurons procedure. Methods Based on two transgenic mouse lines...
Background Cancer pain is a well-known serious complication in metastatic or terminal cancer patients. Current management remains unsatisfactory. The activation of spinal and supraspinal P2X 7 receptors plays crucial role the induction maintenance mechanisms various kinds acute chronic pain. midbrain periaqueductal gray vital site endogenous descending pain-modulating system. Tramadol synthetic, centrally acting analgesic agent that exhibits considerable efficacy clinically relieving purpose...
Three new benzylphenanthrenes, named 1-(p-hydroxybenzyl)-4,7-dimethoxyphenanthrene-2-ol (1), 1-(p-hydroxybenzyl)-4,7-dimethoxyphenanthrene-2,8-diol (2), and 1-(p-hydroxybenzyl)-4,7-dimethoxyphenanthrene-2,6-diol (3), along with a known analog were isolated from tubers of Bletilla striata. The structures these compounds established by means HR-ESI-MS, 1D, 2D NMR.
Abstract: Mechanisms of postherpetic neuralgia (PHN) are still not clear. Transcripts such as microRNA (miRNA) and circular RNA (circRNA) in the affected skin may take part initiation development this neuropathic pain; however, their expression profiles skins PHN patients have been reported. The mirror were collected subjected to miRNA circRNA microarray, comparatively analyzed. There 317 differently expressed miRNAs compared with (fold change ≥2.0), 13 them showed fold >10 skin. Only one...
Abstract Aims The claustrum has long been regarded as a vital center for conscious control. Electrical stimulation or damage to the can result in decreased awareness loss of consciousness, suggesting that may be target action general anesthetics. This study aimed determine role propofol anesthesia. Methods We first applied fiber photometry calcium signal recording system record claustral neuronal activity during entire process Chemogenetic activation neurones was then performed verify their...
Xiaochaihutang (XCHT) is one of classic prescriptions in Treatise on Febrile Diseases China which was reported to have the effect anti-hepatic fibrosis vivo. Activation hepatic stellate cells (HSCs) now well established as a central driver liver injury. Nuclear factor erythroid 2-related 2 (Nrf2) an important element for anti-oxidative damage key factors responsible occurrence. This study investigate XCHT compound serum HSCs activation and focus Nrf2 pathway. Rats treatment groups were given...
Background: Astrocytes are now considered as crucial modulators of neuronal synaptic transmission. General anesthetics have been found to inhibit astrocytic activities, but it is not clear whether general within the clinical concentration range affects astrocyte-mediated regulation. Methods: The effects propofol, dexmedetomidine, and ketamine clinically effective ranges on slow inward currents (SICs) were tested by using whole-cell recording in acute prefrontal cortex (PFC) slice...
Treatment of central nervous system (CNS) demyelination is greatly hindered by lack the knowledge regarding to underlying molecular mechanisms as well therapeutic agents. Here, we report a novel small molecule agent, gastrodin (GAS), which can significantly promote CNS myelination in vivo mice models. By using high-throughput sequencing analysis, discover key long non-coding RNA Gm7237 that enhance and up-regulated GAS. Through bioinformatic analysis experimental validations, further unravel...
The aim of the current study was to explore role basal forebrain (BF) in propofol anaesthesia.In present study, we observed neural activities BF during anaesthesia using calcium fibre photometry recording. Subsequently, ibotenic acid injected into verify anaesthesia. Finally, test whether GABAA receptors were involved modulating anaesthesia, muscimol (GABAA receptor agonist) and gabazine antagonist) microinjected BF. Cortical electroencephalogram (EEG), time loss righting reflex (LORR),...
Nowadays, reperfusion is still the most effective treatment for ischemic heart disease. However, cardiac therapy would lead to injury, which may have resulted from endoplasmic reticulum stress (ERS) during reperfusion. Diazoxide (DZ) a highly selective mitochondrial adenosine triphosphate-sensitive potassium channel opener. Its protective effect on I/R injury has been confirmed in many organs such as and brain. mechanism of its not fully elucidated. MicroRNAs (miRNAs) are widely involved...
Significance Fast transmission of nerve impulses with energetic efficiency along axons cells is essential for brain function. Toward this goal, are wrapped by the myelin membranes extended from oligodendrocytes, which known as myelination axons. Therefore, dependent on formation oligodendrocyte lineage cells. How formed remains incompletely known. Previous studies suggest that signaling pathways growth factors and amino acids might coordinate regulation formation, but specific acid pathway...