A5071548616- Gut microbiota and health
- Phytoplasmas and Hemiptera pathogens
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Fungal Biology and Applications
- Metabolomics and Mass Spectrometry Studies
- Cocoa and Sweet Potato Agronomy
- Insect-Plant Interactions and Control
- Food composition and properties
- Nutritional Studies and Diet
- Bone health and osteoporosis research
- Metabolism and Genetic Disorders
- Infectious Encephalopathies and Encephalitis
- Polysaccharides and Plant Cell Walls
- Infant Nutrition and Health
- Phytochemicals and Antioxidant Activities
- Research on scale insects
- Medicinal Plants and Bioactive Compounds
- Plant Physiology and Cultivation Studies
- Bacterial Infections and Vaccines
- Escherichia coli research studies
- Vitamin D Research Studies
- Postharvest Quality and Shelf Life Management
- Plant Pathogenic Bacteria Studies
- Rabies epidemiology and control
University of California, Davis
2014-2025
Kyiv City Clinical Oncology Center
2019
Arizona State University
2009
Abstract Background Bifidobacterium longum subsp. infantis ( B. ) is a commensal bacterium that colonizes the gastrointestinal tract of breast-fed infants. can efficiently utilize abundant supply oligosaccharides found in human milk (HMO) to help establish residence. We hypothesized metabolites from grown on HMO produce beneficial effect host. Results In previous study, we demonstrated routinely dominated fecal microbiota breast fed Bangladeshi infant cohort (1). Characterization metabolome...
Pyrroloquinoline quinone (PQQ) influences energy-related metabolism and neurologic functions in animals. The mechanism of action involves interactions with cell signaling pathways mitochondrial function. However, little is known about the response to PQQ humans. Using a crossover study design, 10 subjects (5 females, 5 males) ingested added fruit-flavored drink two separate studies. In 1, was given single dose (0.2 mg PQQ/kg). Multiple measurements plasma urine levels changes antioxidant...
Scope We examined the intestinal and systemic responses to incorporating a type 2 resistant starch (RS) into high fat diet fed obese mice. Methods results Diet‐induced obese, C57BL/6J male mice were an HF without or with 20% (by weight) high‐amylose maize (HF‐RS) for 6 weeks. Serum adiponectin levels higher RS consumption, but there no differences in weight gain adiposity. With HF‐RS, expression of ileal TLR2 Reg3g cecal occludin, TLR2, TLR4, NOD1 NOD2 induced; whereas colonic concentrations...
Huanglongbing (HLB), considered the most serious citrus disease in world, is associated with nonculturable bacterium 'Candidatus Liberibacter asiaticus' (Las). Infection of by this pathogen leads to reduced plant vigor and productivity, ultimately resulting death infected tree. It can take up two years following initial infection before outward symptoms become apparent, making detection difficult. The existing knowledge gap our understanding Las its pathogenesis leading HLB has stymied...
Huanglongbing (HLB), also known as Citrus Greening Disease, is caused by the bacterium 'Candidatus Liberibacter asiaticus' (CLas) and a serious threat to citrus industry. To understand effect of CLas infection on metabolome, juice from healthy (n = 18), HLB-asymptomatic HLB-symptomatic Hamlin well 18) Valencia sweet oranges (from southern eastern Florida) were evaluated using (1)H NMR-based metabolomics. Differences in concentration several metabolites including phenylalanine, histidine,...
Rabies is a rapidly progressive lyssavirus encephalitis that statistically 100% fatal. There are no clinically effective antiviral drugs for rabies. An immunologically naïve teenager survived rabies in 2004 through improvised supportive care; since then, 5 additional survivors have been associated with use of the so-called Milwaukee Protocol (MP). The MP applies critical care focused on altered metabolic and physiologic states aim this study was to examine profile cerebrospinal fluid (CSF)...
Huanglongbing (HLB) is a severe, incurable citrus disease caused by the bacterium 'Candidatus Liberibacter asiaticus' (CLas). Although leaves serve as site of initial infection, CLas known to migrate and colonize root system; however, little about impact infection on metabolism resident microbial communities. Scions 'Lisbon' lemon 'Washington Navel' orange grafted onto 'Carrizo' rootstock were with either CLas-infected budwood or uninfected budwood. Roots obtained from trees 46 weeks after...
"Candidatus Liberibacter asiaticus" (CLas) is the bacterium associated with citrus disease Huanglongbing (HLB). Current CLas detection methods are unreliable during presymptomatic infection, and understanding pathogenicity to help develop new techniques challenging because has yet be isolated in pure culture. To understand how affects metabolism whether infected plants produce systemic signals that can used improved techniques, leaves from Washington Navel orange (Citrus sinensis (L.)...
Obesity is a prevalent chronic condition in many developed and developing nations that raises the risk for heart disease, stroke, diabetes. Previous studies have shown consuming particular probiotic strains of Lactobacillus associated with improvement obese diabetic phenotype; however, mechanisms these beneficial effects are not well understood. In this study, C57BL/6J male mice were fed lard-based high fat diet 15 weeks plantarum supplementation NCIMB8826 (Lp) between 10 (n = per group)....
Background Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis treatment. Metabolomics cerebrospinal fluid (CSF) was explored as a method differentiating among neurological diseases causing EM using single CSF sample. Methodology/Principal findings 1H NMR metabolomics applied samples from 27 patients with laboratory-confirmed disease, including Lyme disease or West Nile Virus...
The effects of elevation, rootstock, and soil depth on the nutritional quality mandarin oranges from 11 groves in California were investigated by nuclear magnetic resonance (NMR) spectroscopy quantifying 29 compounds applying multivariate statistical data analysis. A comparison juice with deeper trifoliate rootstock versus those shallow C-35 revealed differences concentrations 4-aminobutyrate, ethanol, phenylalanine, succinate, isoleucine. fruit trees grown at higher lower elevation that had...
Scope The soy isoflavone, daidzein, is metabolized by gut microbiota to O ‐desmethylangolensin (ODMA) and/or equol. Producing equol postulated as a contributing factor for the beneficial effects of soy. Methods and results This randomized, controlled, cross‐over design used an untargeted metabolomic approach assess metabolic profile different daidzein metabolizers. Adults ( n = 17) with cardiometabolic risk factors received nuts or control food 4 weeks, separated 2‐week washout. No...
Human milk offers significant health benefits for infants; however, when not feasible, infant formula serves as an alternative. The higher protein content in is thought to contribute the distinct metabolic profiles observed formula-fed infants compared those fed human milk. This study investigates impact of quantity and whey types on serum, urine, fecal metabolomes infants. A secondary analysis was performed a random subset 200 well-characterized per-protocol who completed prospective,...
Although there is great interest in the specific mechanisms of how gut microbiota modulate biological processes human host, extent host-microbe interactions and bacteria-specific metabolic activities for survival co-evolved gastrointestinal environment remain unclear. Here, we demonstrate a comprehensive comparison host epithelial response induced by either pathogenic or commensal strain Escherichia coli using multi-omics approach. We show that Caco-2 cells incubated with E. display an...
Alendronate sodium (Fosamax) is most widely used for the prevention and treatment of osteoporosis. It a type anti-resorptive agent that reduces risk fractures by changing bone turnover mineral density. We investigated effect Fosamax on mouse model Twenty-seven female C57BL/6JNarl mice were divided into three groups: sham, ovariectomized (OVX) OVX + (Fosamax). After 23 weeks, density femurs was analyzed using microcomputed tomography (micro-CT), serum osteoblast osteoclast activity, as well...
The water-soluble metabolites in 5 mushrooms were identified and quantified using proton nuclear magnetic resonance (NMR) spectroscopy software for targeted metabolite detection quantification. In total, 35 compounds found Agaricus brasiliensis, 25 Taiwanofungus camphoratus, 23 Ganoderma lucidum (Taiwan) Lentinus edodes, 16 G. (China). Total amounts of all A. T. lucidum, (China), L. edodes 149,950.51, 12,834.18, 9,549.09, 2,788.41, 111,726.51 mg/kg dry weight, respectively. These categorized...
Comparison of the metabolic changes prior to symptom development upon infection with Candidatus Liberibacter asiaticus (CLas), bacterium associated citrus greening disease, between hosts different tolerances is lacking. The objective this study was compare early response Lisbon lemon (Citrus limon) and Washington navel orange sinensis [L.] Osbeck), two species commercially important California, CLas through graft inoculation. Here, we transcriptome, proteome, metabolome response, using RNA...
Little is known about how combining a probiotic with prebiotic dietary fiber affects the ability of either biotic to improve health. We hypothesized that prebiotic, high-amylose maize type 2-resistant starch (RS) together Lactiplantibacillus plantarum NCIMB8826 (LP) as complementary synbiotic results in additive effects on gut microbiota diet-induced obese mice and other body sites. Diet-induced C57BL/6J male were fed high-fat diet adjusted contain RS (20% by weight), LP (109 cells every 48...
Obesity, type 2 diabetes mellitus, and metabolic syndrome are associated with a dysbiotic microbiome unregulated immune activation. Thus, identifying dietary strategies enriching for members of the microbiota that correct these deficiencies in indigenous is useful managing preventing those chronic conditions. This approach holds particular promise consumption strains Lactobacillus species commonly consumed fermented foods. Herein, we investigated effects regular plantarum (LP) on both...
To understand and characterize the pathogenic mechanisms of inflammatory bowel disease, dextran sulfate sodium (DSS) has been used to induce acute chronic colitis in animal models by causing intestinal epithelium damage. The mechanism action DSS producing this outcome is not well understood. In an effort how might impact epithelial cell metabolism, we studied line Caco-2 incubated with 1% over 56 hours using 1H NMR spectroscopy. We observed no difference viability as compared control...
Nuclear magnetic resonance (NMR) spectroscopy has been used to obtain the profile of soluble metabolites on fungus. To enhance ergothioneine content in Pleurotus citrinopileatus mycelium, amino acid precursors were for submerged fermentation. The study aimed analyze high-ergothioneine and regular P. mycelia (HEPM RPM) basidiomata (PCB) using NMR spectroscopy. Principal component analysis (PCA) was applied differentiate describe differences among three sets metabolites. results showed that 55...