A5040304325- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Neurological disorders and treatments
- Nerve injury and regeneration
- Bone and Dental Protein Studies
- Barrier Structure and Function Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Alzheimer's disease research and treatments
- Proteoglycans and glycosaminoglycans research
- Nuclear Receptors and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Corneal Surgery and Treatments
- Hedgehog Signaling Pathway Studies
- Reproductive Biology and Fertility
- Neuroscience and Neural Engineering
- Corneal surgery and disorders
- CRISPR and Genetic Engineering
- Blood transfusion and management
- Genetics and Neurodevelopmental Disorders
- Blood donation and transfusion practices
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
- 3D Printing in Biomedical Research
- Protein Degradation and Inhibitors
- Connective tissue disorders research
Hospital Juárez de México
2019-2025
Universidad Nacional Autónoma de México
2016-2024
Instituto Nacional de Neurología y Neurocirugía
2016-2024
Instituto Politécnico Nacional
2010-2020
Center for Research and Advanced Studies of the National Polytechnic Institute
2013-2014
Transit of human neural stem cells, ReNcell CX, through the blood brain barrier (BBB) was evaluated in an vitro model BBB and nude mice. The based on rat microvascular endothelial cells (RBMECs) cultured Millicell inserts bathed from basolateral side with conditioned media (CM) astrocytes or glioma C6 cells. Glioma CM induced a significant transendothelial migration ReNcells CX comparison to astrocyte CM. presence high amounts HGF, VEGF, zonulin PGE2, together low abundance EGF, promoted...
Cell therapy in experimental models of Parkinson's disease replaces the lost dopamine neurons (DAN), but we still need improved methods to guide dopaminergic axons (DAx) grafted make proper connections. The protein Semaphorin 3C (Sema3C) attracts DAN and enhances their growth. In this work, show that hydrogel PuraMatrix, a self-assembling peptide-based matrix, incorporates Sema3C releases it steadily during 4 weeks. We also tested if hydrogel-delivered DAx using system rat midbrain explants...
Chromatin architecture influences transcription by modulating the physical access of regulatory factors to DNA, playing fundamental roles in cell identity. Studies on dopaminergic differentiation have identified coding genes, but relationship with non-coding genes or chromatin accessibility remains elusive. Using RNA-Seq and ATAC-Seq we profiled differentially expressed transcripts open regions during early neuron differentiation. Hierarchical clustering resulted 6 groups unique...
Two siblings from a Mexican family who carried lethal Raine syndrome are presented. A newborn term male (case 1) and his 21 gestational week brother 2), with similar osteosclerotic pattern: generalized osteosclerosis, which is more evident in facial bones cranial base. Prenatal findings at weeks histopathological features for case 2 described. novel combination of biallelic FAM20C pathogenic variants were detected, maternal cytosine duplication position 456 paternal deletion 474 exon 1,...
Severe corneal inflammation produces opacity or even perforation, scarring, and angiogenesis, resulting in blindness. In this study, we used the cornea to examine effect of new anti-angiogenic chemopreventive agents. We researched two extracts, methanol (Met) hexane (Hex), from seed Cucurbita argyrosperma, on inflamed corneas. The corneas Wistar rats were alkali-injured treated intragastrically for seven successive days. evaluated: score, neovascularization (CNV) area, re-epithelialization...
Human embryonic stem cells (hESCs) differentiate into specialized cells, including midbrain dopaminergic neurons (DANs), and Non-human primates (NHPs) injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine develop some alterations observed in Parkinson's disease (PD) patients. Here, we obtained well-characterized DANs from hESCs transplanted them two parkinsonian monkeys to assess their behavioral imaging changes. expressed markers, generated action potentials, released dopamine (DA)...
Parkinson’s disease (PD) is characterized by the progressive loss of midbrain dopaminergic neurons (DaNs) substantia nigra pars compacta and decrease dopamine in brain. Grafting DaN differentiated from embryonic stem cells (ESCs) has been proposed as an alternative therapy for current pharmacological treatments. Intrastriatal grafting such DaNs mouse or human ESCs improves motor performance, restores DA release, suppresses receptor super-sensitivity. However, a low percentage grafted survive...
HoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses and Enhanced Green Fluorescent Protein (EGFP), control with stable expression EGFP. The resulting H9-HoxA9-EGFP H9-EGFP cell lines allow efficient visualization hESCs by fluorescent microscopy, quantification flow cytometry differentiation tracking. Both maintained pluripotent phenotype, ability...
Introduction. Biological adhesives and effective topical therapeutic agents that improve wound healing are urgently required for the treatment of chronic ulcers. A biodegradable adhesive based on a carbohydrate polymer with zinc oxide (CPZO) was shown to possess anti-inflammatory activity enhance healing, but its bactericidal unknown.Aim. To investigate CPZO against bacteria commonly present as infectious in wounds.Methodology. We examined three biofilm-producing (Staphylococcus aureus,...
Cornea severe inflammation produces opacity or even perforation, scarring, and angiogenesis, resulting in blindness. The cornea can be used to study the effect of new anti-angiogenic chemopreventive agents. We researched two extracts, Methanol (Met) Hexane (Hex), from seed Cucurbita argyrosperma, inflamed corneas. corneas Wistar rats were alkali-injured treated intragastrically for seven successive days. Clinical manifestation as score, corneal neovascularization (CNV) area,...
During midbrain development, dopamine neuron differentiation occurs before birth. Epigenetic processes such as DNA methylation and demethylation well post-translational modification of histones occur during neurogenesis. Here, we administered histamine (HA) into the brain E12 embryos in vivo observed significant lower immunoreactivity Lmx1a+ Tyrosine Hydroxylase (TH)+ cells, with parallel decreases expression early (Lmx1a, Msx1) late (Th) dopaminergic (mDA) genes. With MeDIP assays found...
FAM20C (family with sequence similarity 20, member C) is a serine/threonine-specific protein kinase that ubiquitously expressed and mainly associated biomineralization phosphatemia regulation. It mostly known due to pathogenic variants causing its deficiency, which results in Raine syndrome (RNS), sclerosing bone dysplasia hypophosphatemia. The phenotype recognized by the skeletal features, are related hypophosphorylation of different bone-target proteins. However, has many targets,...
Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons in substantia nigra pars compacta, which results a prominent reduction striatal dopamine levels leading to motor alterations. The mechanisms underlying neurodegeneration PD remain unknown. Here, we generated an induced pluripotent stem cell line from dermal fibroblasts Mexican patient diagnosed with sporadic (UNAMi002-A) and another carrying point mutation c.1423delC PINK1 (UNAMi003-A). These...