A5022391082- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Pneumonia and Respiratory Infections
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Enterobacteriaceae and Cronobacter Research
- Tuberculosis Research and Epidemiology
- Infant Development and Preterm Care
- Respiratory and Cough-Related Research
- Microbial Community Ecology and Physiology
- Oral microbiology and periodontitis research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- Quinazolinone synthesis and applications
- Child Nutrition and Feeding Issues
- Legionella and Acanthamoeba research
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2018-2021
University of Milan
2018-2021
University of Pavia
2018-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2021
Ospedale Maggiore
2021
National Interuniversity Consortium of Materials Science and Technology
2018
Istituti Clinici Scientifici Maugeri
2018
Introduction Neutrophil elastase activity in sputum can identify patients at high risk of airway infection and exacerbations bronchiectasis. Application this biomarker clinical practice is limited, because no point-of-care test available. We tested whether a novel semi-quantitative lateral flow device (neutrophil stick – NEATstik®) stratify bronchiectasis according to severity, exacerbation risk. Methods Sputum samples from 124 with stable enrolled the UK Spain were using NEATstik®, which...
Recently a frequent exacerbator phenotype has been described in bronchiectasis, but the underlying biological mechanisms are unknown. Antimicrobial peptides (AMPs) important host defence against microbes can be proinflammatory chronic lung disease.To determine pulmonary and systemic levels of AMP their relationship with disease severity future risk exacerbations bronchiectasis.A total 135 adults bronchiectasis were prospectively enrolled at three European centres. Levels cathelicidin LL-37,...
Introduction Neutrophilic inflammation is a major driver of bronchiectasis pathophysiology, and neutrophil elastase activity the most promising biomarker evaluated in sputum to date. How active correlates with lung microbiome still unexplored. We aimed understand whether associated low microbial diversity distinct characteristics. Methods An observational, cross-sectional study was conducted at programme Policlinico Hospital Milan, Italy, where adults were enrolled between March 2017 2019....
Although bronchiectasis pathophysiology has been historically understood around the presence of airway neutrophilic inflammation, recent experiences are consistent with identification a type 2 inflammation (T2) high endotype in bronchiectasis. In order to evaluate prevalence and clinical characteristics patients T2-high explore their response biologicals, two studies were carried out. cross-sectional study, adults without asthma underwent clinical, radiological, microbiological assessment,...
Different steps and conditions for DNA extraction microbiota analysis in sputum have been reported the literature. We aimed at testing both dithiothreitol (DTT) enzymatic treatments of samples identifying most suitable technique sputum. Sputum with without DTT were compared terms their median levels coefficient variation between replicates yield real-time PCR 16S rRNA gene. Treatments lysozyme lysostaphin
Traditionally regarded as single cell organisms, bacteria naturally and preferentially build multicellular communities that enable them to react efficiently external stimuli in a coordinated fashion with extremely effective outcomes. These are bacterial biofilms, where cells or microcolonies embedded self-built Extracellular Polymeric Substance (EPS), composed of different macromolecules, e.g., polysaccharides, proteins, lipids, extracellular DNA (eDNA). Despite being the most common form...
The analysis of microbiome in respiratory samples is a topic great interest chronic diseases. method used to prepare sputum for very heterogeneous. selection the most suitable methodology DNA extraction fundamental have representative data. objective this study was compare different conditions from adult patients with bronchiectasis. Five bronchiectasis were collected at Policlinico Hospital Milan, Italy. Eighteen compared, including two enzyme-based (Roche and Zymo) one beads-based (Mobio)...
Background: Recurrent acute otitis media (RAOM) is common in children, and it may result spontaneous tympanic membrane perforation (STMP), management of which often challenging. In the upper respiratory tract (URT), resident microorganisms play a pivotal role pathogenesis prevention, as they are able to inhibit colonization process otopathogens growth. particular, Dolosigranulum spp. Corynebacterium spp . have been associated with health several studies. This study aims at comparing both...
A substantial increase in pulmonary and extra-pulmonary diseases due to non-tuberculous mycobacteria (NTM) has been documented worldwide, especially among subjects suffering from chronic respiratory immunocompromised patients. Many questions remain regarding the epidemiology of disease NTM (NTM-PD) mainly because reporting NTM-PD health authorities is not mandated several countries, including Italy. This manuscript describes protocol first Italian registry adult patients with infections...
<b>Introduction:</b> There is no consensus on which the best method to extract bacterial DNA from sputum. We aimed at comparing 20 different extraction techniques both mock community and sputum samples. <b>Methods:</b> One was built 5 sputa cystic fibrosis bronchiectasis patients were evaluated Policlinico Hospital in Milan, Italy. for compared, including two enzyme-based (Roche Zymo) beads-based (Mobio QIAGEN) tested with/without Dithiothreitol (DTT) lysostaphin (0.18 0.36 mg/mL) steps....
Bronchiectasis refers to both the name of a disease and single radiological appearance that may, or may not, be associated with disease. As chronic respiratory disease, bronchiectasis is characterized by variable range signs symptoms overlap other conditions. The proper identification as in primary secondary care paramount importance. However, standardized definition radiologically clinically significant still missing. Disease heterogeneity hallmark applies not only features clinical...
Background The reported prevalence of immunodeficiencies in bronchiectasis patients is variable depending on the frequency and extent immunological tests performed. European Respiratory Society guidelines recommend a minimum bundle tests. Broadening spectrum could increase number diagnosed with an immunodeficiency those who receive specific therapy. primary objective present study was to assess performance different sets diagnosing any, primary, secondary or treatable adults bronchiectasis....
<b>Introduction:</b> Neutrophil elastase (NE) activity in sputum can identify patients at high risk of airway infection and exacerbations bronchiectasis. Application this biomarker clinical practice is limited because no point care test available. We tested whether a novel semi-quantitative lateral flow device (Neutrophil stik–NEATstik®) stratify bronchiectasis according to severity, exacerbation risk. <b>Methods:</b> Sputum samples from 124 with stable enrolled the UK Spain were using...
Vitamin D modulates immune responses and its deficiency has been observed in more than 60% of bronchiectasis patients. binding protein (DBP) is coded by the GC gene, involved transport vitamin D, includes a number isoforms based on single nucleotide polymorphisms (SNPs) coding region at rs7041 rs4855. We evaluated possible clinical impact DBP an observational, cross-sectional study conducted 116 patients, who were genetically characterized for rs4588 SNPs. Results showed that GC1f isoform...
<b>Introduction:</b> Airway colonization by potentially pathogenic bacteria (PPB) worsens outcomes in bronchiectasis. Antimicrobial peptides (AMP) secreted epithelial cells, neutrophils and macrophages protect against exogenous agents including bacteria. The objective of the study was to determine relationship between pulmonary systemic AMP levels presence airway PPB <b>Methods:</b> Adult patients with clinically stable bronchiectasis were prospectively enrolled at three European centers...
Background: Bronchiectasis is the final result of different processes and most guidelines advocate for a careful evaluation those etiologies which might be treated or change patients’ management, including cystic fibrosis (CF). Main body: CFTR mutations have been reported with higher frequency in bronchiectasis population. Although ruling out CF considered as main step etiological screening bronchiectasis, testing lacks standardized approach both from research clinical point view. In this...
Background: Bronchiectasis is predominantly a neutrophilic inflammatory disease. There are no established therapies that directly target inflammation because of lack understanding the underlying mechanisms leading to severe Neutrophil extracellular trap (NET) formation mode host defence has been implicated in multiple diseases.Methods: We conducted series UK and international studies investigate role NETs disease severity treatment response bronchiectasis. Nano LC/MS-MS was used identify...
<b>Introduction:</b> International guidelines suggest a limited bundle of tests to investigate Bx etiology. We aimed evaluate an extensive performed on every patient increase the chances identify treatable <b>Methods:</b> A prospective, observational study was conducted at Policlinico Hospital in Milan, Italy, from 2014 2017. All consecutive adults with underwent following tests: CBC, Aspergillus IgG and IgE, serum electrophoresis, sweat test genetics for cystic fibrosis, immunoglobulins,...