A5003642259- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Eicosanoids and Hypertension Pharmacology
- Stress Responses and Cortisol
- Sodium Intake and Health
- Circadian rhythm and melatonin
- Blood Pressure and Hypertension Studies
- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Dietary Effects on Health
- Hemoglobinopathies and Related Disorders
- Neuroendocrine regulation and behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cystic Fibrosis Research Advances
- Pregnancy and preeclampsia studies
- Chronic Kidney Disease and Diabetes
- Pediatric health and respiratory diseases
- Receptor Mechanisms and Signaling
- Histone Deacetylase Inhibitors Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neuroscience of respiration and sleep
- Tracheal and airway disorders
- Atherosclerosis and Cardiovascular Diseases
University of Dundee
2019-2025
University of Alabama at Birmingham
2016-2025
Metabolism and Renal Physiology
2017-2025
Ninewells Hospital
2021-2024
University of California, Santa Barbara
2022-2024
Pediatric Nephrology of Alabama
2019-2022
University of Alabama
2022
Augusta University
2010-2020
Prevention Institute
2000-2020
Artifex University
2020
The particulate enzyme responsible for the synthesis of endothelium-derived relaxing factor has been purified from cultured and native (noncultured) bovine aortic endothelial cells. Purification solubilized preparation by affinity chromatography on adenosine 2',5'-bisphosphate coupled to Sepharose followed Superose 6 gel filtration resulted in a single protein band after denaturing polyacrylamide electrophoresis that corresponded approximately 135 kDa. activity various fractions was assayed...
Nitric oxide synthases (NOS Types I-III) generate nitric (NO), which in turn activates soluble guanylyl cyclase (GC-S). The distribution of this NO-mediated (nitrinergic) signal transduction pathway the body is unclear. A polyclonal monospecific antibody to rat cerebellum NOS-I and a monoclonal lung GC-S were employed localize protein components different organs tissues. We confirmed localization neurons central peripheral nervous system, where NO may regulate cerebral blood flow mediate...
Endothelium-derived relaxing factor/nitric oxide (EDRF/NO) synthesized by bovine aortic endothelial cells and subcellular fractions thereof was assayed its stimulating effect on soluble guanylyl cyclase of rat fetal lung fibroblasts (RFL-6 cells). The release EDRF/NO intact could be stimulated with bradykinin, thrombin, or ADP abolished in Ca2(+)-free medium. When were analyzed, some EDRF/NO-synthesizing activity found the cytosolic fraction, but most associated particulate fraction. Both...
Complementary DNA clones corresponding to human brain nitric oxide (NO) synthase have been isolated. The deduced amino acid sequence revealed an overall identity with rat NO of about 93% and contained all suggested consensus sites for binding the co‐factors. cDNA transfected COS‐1 cells showed significant activity typical co‐factor requirements. Unexpectedly, messenger RNA levels this isoform was more abundant in skeletal muscle than brain. Moreover, we detected high expressed protein by...
Abstract Atherosclerosis is associated with reduced endothelium-derived relaxing factor bioactivity. To determine whether this due to decreased synthesis of nitric oxide synthase (NOS), we examined normal and atherosclerotic human vessels by in situ hybridization immunocytochemistry using probes specific for endothelial (ecNOS), inducible (iNOS), neuronal (nNOS) NOS isoforms. ecNOS was detected cells overlying aortas, fatty streaks, advanced lesions. A comparison the relative expression von...
The soluble form of guanylyl cyclase-activating-factor (GAF) synthase from rat cerebellum was purified to homogeneity by sequential affinity chromatographic steps on adenosine 2',5'-bisphosphate (2',5'-ADP)-Sepharose and calmodulin-agarose. Enzyme activity during purification bioassayed the L-arginine-, NADPH-, Ca2+/calmodulin-dependent formation a plasma membrane-permeable nitric oxide-like factor that stimulated cyclase in RFL-6 cells. With calmodulin NADPH as cofactors, GAF induced an...
The purposes of this study were to assess the long-term effect adverse childhood experiences (ACEs) on blood pressure (BP) trajectories from young adulthood and examine whether relation is explained by socioeconomic status (SES) or risk behaviors that are associated with ACEs.Systolic diastolic BPs measured up 16 times (13 average) over a 23-year period in 213 African Americans 181 European 5 38 years age. Retrospective data traumatic before 18 age collected, including abuse, neglect,...
Plasma levels of IL-6 correlate with high blood pressure under many circumstances, and ANG II has been shown to stimulate production from various cell types. This study tested the role in mediating hypertension caused by high-dose a high-salt diet. Male C57BL6 knockout (IL-6 KO) mice were implanted biotelemetry devices placed metabolic cages measure mean arterial (MAP), heart rate (HR), sodium balance, urinary albumin excretion. Baseline MAP during control period averaged 114 +/- 1 109 mmHg...
We have produced specific monoclonal antibodies (MAb) against particulate bovine aortic endothelial nitric oxide synthase. In Western blots, native and cultured cells as well microvascular possess immunoreactive NO dot MAb H210 H32 detect 1 ng 100 pg of purified synthase, respectively. Both are to the synthase do not cross-react with other known isoforms namely from brain, cytokine/endotoxin-induced macrophages, or vascular smooth muscle cells. Immunohistochemical studies demonstrated...
Endothelin (ET) receptor blockade delays the progression of diabetic nephropathy; however, mechanism this protection is unknown. Therefore, aim study was to test hypothesis that ET(A) attenuates superoxide production and inflammation in kidney rats. Diabetes induced by streptozotocin (diabetic rats with partial insulin replacement maintain modest hyperglycemia [HG]), sham received vehicle treatments. Some also ETA antagonist ABT-627 (sham+ABT HG+ABT; 5 mg/kg per d; n = 8 10/group). During...
The purpose of this study was to measure the temporal profile expression endothelial nitric oxide synthase (NOS) in cerebral microvessels after middle artery occlusion rat. Middle performed on 24 male Wistar rats by extracranial insertion a 4-0 nylon monofilament into internal artery. Three additional were used as controls. Animals killed at 1, 2, 4, 6, 24, 48, 72, and 168 hours (n = 3 per time point). Rat brains perfused with buffer, frozen, sectioned, stained monoclonal antibody against...
Besides differential methylation, DNA methylation variation has recently been proposed and demonstrated to be a potential contributing factor cancer risk. Here we aim examine whether variability in is also an important feature of obesity, typical non-malignant common complex disease. We analyzed genome-wide profiles over 470,000 CpGs peripheral blood samples from 48 obese lean African-American youth aged 14–20 y old. A substantial number differentially variable CpG sites (DVCs), using...