A5001770817- Spine and Intervertebral Disc Pathology
- Medical Imaging and Analysis
- Spinal Fractures and Fixation Techniques
- Musculoskeletal pain and rehabilitation
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Bone health and treatments
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Scoliosis diagnosis and treatment
- Bone and Joint Diseases
- Shoulder Injury and Treatment
- Spinal Cord Injury Research
- Bone Tissue Engineering Materials
- Orthopedic Surgery and Rehabilitation
- Elbow and Forearm Trauma Treatment
- RNA Interference and Gene Delivery
- Cervical and Thoracic Myelopathy
- Nanoplatforms for cancer theranostics
- Natural product bioactivities and synthesis
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Hip disorders and treatments
- RNA modifications and cancer
Sir Run Run Shaw Hospital
2016-2025
Zhejiang University
2016-2025
Blood Center of Zhejiang Province
2019-2025
Xuzhou Medical College
2025
State Key Laboratory of Modern Optical Instruments
2024
Yichang Central People's Hospital
2009-2012
Second Affiliated Hospital of Zhejiang University
1996
Mitochondrial dysfunction and oxidative stress damage are hallmarks of osteoarthritis (OA). Mesenchymal stem cell (MSC)-derived exosomes important in intercellular mitochondria communication. However, the use MSC for regulating mitochondrial function OA has not been reported. This study aimed to explore therapeutic effect a three dimensional (3D) printed scaffold early therapeutics. Methods: We first examined mitochondria-related proteins normal human cartilage samples investigated whether...
Osteosarcoma (OS) is the most common malignant bone tumor and has a poor prognosis. The potential involvement of circular RNAs (circRNAs) in OS progression remains unexplored. Here, we report that CircECE1, RNA derived from human ECE1, plays critical role energy metabolism OS.The RIP chip sequence assay was performed to confirm through overexpression or knockdown CircECE1 verify its function 143B U2OS. immunoprecipitation were used CircECE1's regulation protein c-Myc co- verified competitive...
Insufficient intracellular anabolism is a crucial factor involved in many pathological processes the body
Abstract Diabetic wound is a significant challenge for clinical treatment with high morbidity and mortality. Plenty of hydrogels good biocompatibility have been widely used in diabetic healing. However, most them cannot be directly absorbed utilized by the wounds, which prolongs regeneration time. Here new type healing hydrogel developed that based on histidine, natural dietary essential amino acid tissue formation. The cross‐linked zinc ions (Zn 2+ ) sodium alginate (SA) via dynamic...
Osteoarthritis is a prevalent age-related disease characterized by dysregulation of extracellular matrix metabolism, lipid and upregulation senescence-associated secretory phenotypes. Herein, we clarify that CircRREB1 highly expressed in secondary generation chondrocytes its deficiency can alleviate FASN related senescent phenotypes osteoarthritis progression. impedes proteasome-mediated degradation inhibiting acetylation-mediated ubiquitination. Meanwhile, induces RanBP2-mediated...
Reactive Oxygen Species (ROS) refers to a highly reactive class of oxidizing species that have the potential induce cellular apoptosis and necrosis. Cuproptosis, type cell death, is primarily associated with effects copper ions. However, specific relationship between ROS, cuproptosis, osteosarcoma (OS) remains relatively unexplored. Additionally, there limited research on use cuproptosis in conjunction oxidative stress for treating OS inhibiting tumor-induced bone destruction. To address...
Abstract Intervertebral disc degeneration (IVDD) is a leading cause of low back pain, with limited effective treatments due to an incomplete understanding disease mechanisms. In this study, we report that circFUNDC1, nuclear circular RNA, markedly downregulated in nucleus pulposus cells (NPCs) from patients end-stage IVDD. CircFUNDC1 derived the gene encoding FUN14 domain-containing 1 (FUNDC1) protein, which essential for mitophagy and cell survival. Functional analyses reveal circFUNDC1...
Background: Multifidus muscle injury and atrophy are common after posterior lumbar spine surgery associated with low back pain functional disability. In theory, muscle-splitting retraction a self-retaining retractor considered to be the major surgical factors. The effects mechanisms of have been well studied, but exact effect possible mechanism still lack experimental evidence. Methods: New Zealand White rabbits were divided into two groups. group S, through skin lumbodorsal fascial...
Aim: We aimed to investigate the proteomics of primary chondrocyte exosomes and effect in osteoarthritis (OA) treatment. Materials & methods: isolated from chondrocytes cultured normal (D0) inflammatory environments induced by IL-1β determined these exosomes. Next, we investigated what mechanism D0 have OA Results: There were more proteins that belonged mitochondrion involved immune system processes Notably, intra-articular administration successfully prevented development OA. could restore...
Osteoarthritis (OA) is a common, age-related, and painful disease characterized by cartilage destruction, osteophyte formation, synovial hyperplasia. This study revealed that circPDE4D, circular RNA derived from human linear PDE4D, plays critical role in maintaining the extracellular cellular matrix (ECM) during OA progression. circPDE4D was significantly downregulated tissues stimulation with inflammatory cytokines. The knockdown of predominantly contributed to Aggrecan loss upregulation...
Abstract Objective We focus on providing the first comprehensive national dataset incidence, injury aetiology and mortality of TSCI in China. Methods A multi-stage stratified cluster sampling method was used. included cases from all hospitals three regions, nine provinces 27 cities China via search electronic medical records retrospectively analysed characteristics 2009 to 2018. estimated incidence total population subgroups. Results There were 5954 actual 2009, corresponding a 45.1 per...
Redundancy of multinucleated mature osteoclasts, which results from the excessive fusion mononucleated preosteoclasts (pOCs), leads to osteolytic diseases such as osteoporosis. Unfortunately, currently available clinical drugs completely inhibit thus interfering with normal physiological bone turnover. pOC-specific regulation may be more suitable for maintaining homeostasis. Here, circBBS9, a previously unidentified circular RNA, was found exert regulatory effects via...
Osteoarthritis (OA) is a degenerative disease with series of metabolic changes accompanied by many altered enzymes. Here, we report that the down-regulated dimethylarginine dimethylaminohydrolase-1 (DDAH1) increased asymmetric (ADMA) in degenerated chondrocytes and OA samples. Global or chondrocyte-conditional knockout ADMA hydrolase DDAH1 accelerated development mice. induces degeneration senescence reduces extracellular matrix deposition, thereby accelerating progression. simultaneously...
Abstract The repair of hierarchical osteochondral defects requires complex gradient reconstruction at different levels, with continuous‐gradient mineralization being crucial. Strategies for achieving have rarely been reported. Here, a mineralized hydrogel is prepared using simple gravitational osmosis ≈2 nm amorphous calcium phosphate nanocluster (ACPC) suspension into composite organic frameworks regeneration. During infiltration, ACPC underwent gradual mineralization, resulting in the...
Nucleus pulposus-like differentiation is always the challenge with application of stem cells for intervertebral disc repair. The combination injectable biomaterials and may provide a resolution this problem, as transmembrane force can affect intracellular environment through integrin αβ. In study, we developed strategy photo-crosslinked gelatin-hyaluronic acid methacrylate (GelHA) hydrogel to commit nucleus adipose stromal (ASCs) ASCs were isolated cultured in GelHA evaluate differentiation....
Therapeutic bioengineering based on stem cell therapy holds great promise in biomedical applications. However, the application of this treatment is limited orthopedics because their poor survival, weak localization, and low retention. In work, magneto-mechanical bioengineered cells consisting magnetic silica nanoparticles (MSNPs) mesenchymal (MSCs) are prepared to alleviate osteoporosis. The MSCs with spatial retention, directional tracking capabilities could be mediated by a guided field...
Autophagy is a protective mechanism in normal cartilage. The present study aimed to investigate the synergistic therapeutic effect of promotion chondrocyte autophagy via exposure cordycepin encapsulated by chitosan microspheres (CM-cordycepin) and photo-crosslinked hyaluronic acid methacrylate (HAMA) hydrogel, with goal evaluating CM-cordycepin as treatment for patients osteoarthritis. First, we developed evaluated characteristics HAMA hydrogels microspheres. Next, measured on cartilage...
Abstract Mechanical force is critical for the development and remodeling of bone. Here we report that mechanical regulates production metabolite asymmetric dimethylarginine (ADMA) via regulating hydrolytic enzyme dimethylaminohydrolase 1 ( Ddah1 ) expression in osteoblasts. The presence -394 4 N del/ins polymorphism higher serum ADMA concentration are negatively associated with bone mineral density. Global or osteoblast-specific deletion leads to increased level but reduced formation....