A5012443625- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Inflammasome and immune disorders
- Fatty Acid Research and Health
- Immune cells in cancer
- Metabolomics and Mass Spectrometry Studies
- Sphingolipid Metabolism and Signaling
- Cancer, Lipids, and Metabolism
- Advanced Glycation End Products research
- Phagocytosis and Immune Regulation
- Tissue Engineering and Regenerative Medicine
- S100 Proteins and Annexins
- Extracellular vesicles in disease
- Traditional Chinese Medicine Analysis
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Pharmacological Effects of Natural Compounds
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Liver physiology and pathology
- RNA Research and Splicing
- Heme Oxygenase-1 and Carbon Monoxide
- Cellular transport and secretion
Yangzhou University
2021-2024
Shanghai Jiao Tong University
2017-2024
Shaanxi Provincial People's Hospital
2024
Shanxi Medical University
2024
Shanghai East Hospital
2020-2023
Tongren Hospital
2021-2022
Beijing Academy of Agricultural and Forestry Sciences
2016-2021
Poultry Research Institute
2016-2021
Capital Medical University
2014-2021
Beijing Obstetrics and Gynecology Hospital
2018
Porcine circovirus type 3 (PCV3), an emerging porcine circovirus, is considered the cause of dermatitis and nephropathy syndrome (PDNS)-like clinical signs other systemic diseases in piglets sows. To evaluate pathogenesis PCV3 infection vivo , we used a virus stock from rescue infectious DNA clone to intranasally inoculate 4- 8-week-old specific-pathogen-free demonstrated successful reproduction PDNS-like disease animals that were inoculated with alone or combined immunostimulation by...
NLR family pyrin domain containing 3 (NLRP3) inflammasome accompanies chronic liver injury and is a critical mediator of inflammation-driven fibrosis. Sphingosine 1-phosphate (S1P)/S1P Receptor (S1PR) signaling participates in fibrogenesis by affecting bone marrow (BM)-derived monocytes/macrophages (BMMs) activation. However, the relationship between S1P/S1PRs NLRP3 BMMs remains unclear. Here, we found significantly elevated gene expression components (NLRP3, pro-interleukin-1β,...
Abstract Inappropriate neutrophil infiltration and subsequent extracellular trap (NET) formation have been confirmed to be involved in chronic inflammatory conditions. Fatty liver disease is an increasingly severe health problem worldwide currently considered the most common cause of disease. Sphingosine 1-phosphate (S1P), a product membrane sphingolipid metabolism, regulates vital physiological pathological actions by inducing activation various cell types through S1P receptors (S1PRs)....
Porcine circovirus type 2 (PCV2) is the etiological agent of PCV2-associated diseases that pose a serious threat to swine industry. PCV2 capsid (Cap) protein has been shown interact with DEAD-box RNA helicase 21 (DDX21), an important regulates virus replication. However, whether interaction between DDX21 and Cap replication remains unclear. Herein, by using western blotting, assays, knockdown analysis, we found infection induced cytoplasmic relocation from nucleolus in cultured PK-15 cells....
Abstract Mechanical force is critical for the development and remodeling of bone. Here we report that mechanical regulates production metabolite asymmetric dimethylarginine (ADMA) via regulating hydrolytic enzyme dimethylaminohydrolase 1 ( Ddah1 ) expression in osteoblasts. The presence -394 4 N del/ins polymorphism higher serum ADMA concentration are negatively associated with bone mineral density. Global or osteoblast-specific deletion leads to increased level but reduced formation....
Nod-like receptor (NLR) family pyrin domain containing 3 (NLRP3) has been regarded as an important initiator or promoter in multiple inflammatory diseases. However, the relationship between cannabinoid 1 (CB1) and macrophage NLRP3 inflammasome corresponding molecular mechanism liver inflammation remain unclear. Mouse injury models were induced by carbon tetrachloride (CCl4) methionine-choline-deficient high fat (MCDHF) diet. Human tissues obtained from patients with different chronic CB1...
Type II alveolar epithelial cell (AECII) apoptosis is one of the most vital causes sepsis-induced acute respiratory distress syndrome (ARDS). Recent evidence has proved that bone mesenchymal stem cell-derived exosomes (BMSC-exos) can effectively reduce ARDS. However, function and molecular mechanism BMSC-exos in AECII remain to be elucidated. In present study, a more significant number apoptosis, high mitochondrial fission p-Drp1 protein levels, low levels biogenesis-related PGC1α, Tfam,...
Abstract Background Myeloid-related protein 8/14 (MRP8/14) is a stable heterodimer formed by two different calcium-binding proteins (MRP8 and MRP14). Studies have identified that MRP8/14 regulates vascular inflammation serves as novel marker of acute coronary syndrome. In this study, we evaluated the correlation between serum levels MRP8/14, hsCRP, endogenous secretory receptor for advanced glycation end-products (esRAGE) occurrence artery disease (CAD), or carotid intima-media thickness...
Growing evidence indicates that SLC3A2 (solute carrier family 3 member 2) is upregulated and correlates with tumor growth in multiple types of cancers, while the role human osteosarcoma (OS) rarely discussed. Thus, aim present study was to demonstrate expression reveal its biological function underlying mechanisms. RT-PCR, western blot analysis immunohistochemistry (IHC) were used assess OS samples cell lines. Cell cycle, Counting Kit-8 (CCK-8) colony formation assays test survival capacity....
Porcine circovirus type 3 (PCV3) infection induces porcine dermatitis and nephropathy syndrome, reproductive failure, multisystemic inflammatory lesions in piglets sows. To better understand the host responses to PCV3 infection, isobaric tags for relative absolute quantification (iTRAQ) labeling combined with LC-MS/MS analysis was used quantitative determination of differentially regulated cellular proteins lungs specific-pathogen-free after 4 weeks infection. Totally, 3429 were detected...
Acute lung injury is a common complication after cardiopulmonary bypass (CPB). α7 Nicotinic acetylcholine receptors (α7nAChR) and α7nAChR-dependent cholinergic signaling are implicated in suppressing the release of high-mobility group box 1 (HMGB1) reducing inflammatory response. A previous study has shown electroacupuncture (EA) pretreatment induces tolerance against injury. However, role EA CPB poorly understood. This used rat model to determine whether was associated with CPB-induced Rats...
Porcine circovirus type 3 (PCV3) is a novel porcine species associated with several diseases such as dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, cardiac pathologies, multisystemic inflammation in piglets sows. Currently, many studies have focused on the interaction between microbiota composition disease progression. However, dynamic changes of gut following PCV3 infection are still unknown. In this study, alterations PCV3-inoculated sham-inoculated...
Porcine circovirus type 4 (PCV4) is an emerging etiological agent which was first detected in 2019. The nucleolar localization signal (NoLS) of PCV4 Cap protein and its binding host cellular proteins are still not elucidated. In the present study, we discovered a distinct novel NoLS Cap, bound to phosphoprotein nucleophosmin-1 (NPM1). serine-48 residue at N-terminal oligomerization domain NPM1 were necessary for Cap/NPM1 interaction. Furthermore, charge property serine position 48 crucial...
Abstract Porcine circovirus type 2 (PCV2), which serves as a major causative agent of PCV2-associated diseases and causes severe loss to the pig industry worldwide, can dysregulate immune response induce immunosuppression in PCV2-infected pigs. Similar PCV2, porcine 3 (PCV3), newly identified swine might be closely associated with dermatitis nephropathy syndrome, reproductive disorder, multisystemic inflammatory responses, also interferes host defense. Interaction between system PCVs is...
Hypervirulent fowl adenovirus serotype 4 (hvFAdV-4) has emerged as a major pathogen of hepatitis-hydropericardium syndrome (HHS) with increased mortality in chickens, resulting economic losses to the Chinese poultry industry since June 2015. Here, we isolated hypervirulent FAdV-4 strain (designated GD616) from 25-day-old meat-type chickens severe HHS Guangdong Province China 2017. The whole genome GD616 shares high homology those recently-reported hvFAdV-4 isolates China, natural deletions...
Background: Although there are concerns regarding their clinical use, embryonic stem cells (ESCs) hold a great promise for cardiac repair. Exosomes deriving from ESCs constitute promising alternative heart restoration. However, effects in hypertension-induced failure still unknown. Objective and Methods: To investigate the of ESCs-derived exosomes on underlying mechanisms, sustained transverse aortic constriction (TAC) was performed 8-week-old C57BL/6 male mice. After 1 months, were isolated...
Entry of virus into cells represents the initiation a successful infection. As an emerging pathogen porcine vesicular disease, clarification process SVV entry enables us to better understand viral life cycle and pathogenesis.
Porcine circovirus type 3 (PCV3) is an emerging pathogen that causes multisystem disease in pigs and poses a severe threat to the swine industry. However, mechanisms of how PCV3 uses host proteins regulate its own life cycle are not well understood. In this study, we found capsid protein interacts with nucleolin degrades it. Degradation by requires recruitment enzyme RNF34, which transported nucleolus from cytoplasm presence protein. Nucleolin also decreases replication promoting release...
Monocyte chemotactic protein-1 (MCP-1) is one of the most representative inflammatory cytokines, and has been proved to be markedly increased in injured liver sphingosine 1-phosphate (S1P)-treated macrophages. However, microRNAs (miRNAs) targeting MCP-1 role miRNA/MCP-1 axis S1P-mediated inflammation remain largely unknown. Here, we demonstrate that expression isolated macrophages MCDHF mice. Moreover, there a positive correlation between hepatic levels S1P MCP-1. We then predict miRNAs by...