A5069034587- Laser-Plasma Interactions and Diagnostics
- Laser-induced spectroscopy and plasma
- Laser-Matter Interactions and Applications
- Nitric Oxide and Endothelin Effects
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Particle Accelerators and Free-Electron Lasers
- Renin-Angiotensin System Studies
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Ocular Surface and Contact Lens
- Epigenetics and DNA Methylation
- Ion Transport and Channel Regulation
- High-pressure geophysics and materials
- Particle accelerators and beam dynamics
- Laser Design and Applications
- Electrolyte and hormonal disorders
- Microbial Metabolic Engineering and Bioproduction
- Renal function and acid-base balance
- Ocular Infections and Treatments
- Natural product bioactivities and synthesis
- Amino Acid Enzymes and Metabolism
- Pregnancy and preeclampsia studies
Hunan University of Arts and Science
2017-2025
Ministry of Agriculture and Rural Affairs
2025
China Agricultural University
2025
Innovation Team (China)
2023-2024
Jilin Academy of Agricultural Sciences
2024
Southwest Petroleum University
2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
Drip Research Technology Services (United States)
2022-2024
Jiangxi Chest Hospital
2018-2023
Chinese Academy of Sciences
2013-2023
Tonic basal release of nitric oxide (NO) by vascular endothelial cells controls blood pressure (BP) in the state. In these studies we investigated effects chronic inhibition NO synthesis rat for a 2-mo period. Significant systemic hypertension developed chronically NO-blocked rats compared to controls. Marked renal vasoconstriction was also observed with elevations glomerular (PGC) and reductions capillary ultrafiltration coefficient (Kf). Chronically develop proteinuria sclerotic injury...
We report on near-GeV electron beam generation from an all-optical cascaded laser wakefield accelerator (LWFA). Electron injection and acceleration are successfully separated controlled in different LWFA stages by employing two gas cells filled with a $\mathrm{He}/{\mathrm{O}}_{2}$ mixture pure He gas, respectively. Electrons Maxwellian spectrum, generated the first assisted ionization-induced injection, were seeded into second 3-mm-thick cell accelerated to be 0.8-GeV quasimonoenergetic...
Genetic transformation of bacteria harboring multiple Restriction-Modification (R-M) systems is often difficult using conventional methods. Here, we describe a mimicking-of-DNA-methylation-patterns (MoDMP) pipeline to address this problem in three difficult-to-transform bacterial strains. Twenty-four putative DNA methyltransferases (MTases) from these strains were cloned and expressed an Escherichia coli strain lacking all the known R-M orphan MTases. Thirteen MTases exhibited modification...
The thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized efficient safe cell factory in fermentative productions of chemicals, biofuels other bio-products. However, it difficult to genetically engineer the deficiency versatile genome editing technology.In this study, we developed a CRISPR-Cas9-assisted multiplex (CMGE) approach including genes knock-outs, multi-locus...
Nephron function is stabilized by tubuloglomerular feedback (TGF). TGF operates within the juxtaglomerular apparatus, sensing changes in tubular flow and eliciting compensatory single nephron GFR (SNGFR). The mediator(s) of remains unconfirmed. One theory that ATP consumed active transport macula densa leads to formation adenosine, which causes glomerular vasoconstriction. We performed micropuncture rats test this hypothesis. Adenosine activity was manipulated microperfusing nephrons with...
During systemic acute blockade of endogenous endothelial-derived relaxing factor (EDRF) with N-monomethyl-L-arginine (NMA), a significant rise in arterial blood pressure (BP) occurred the anesthetized rat. Renal vasoconstriction was also seen, complex changes glomerular hemodynamics; both preglomerular (RA) and efferent arteriolar (RE) resistances increased, producing fall plasma flow (QA) (PGC). The capillary ultrafiltration coefficient (Kf) reduced. net effect small single-nephron...
Oxidative stress and inflammation are well-documented pathological factors in alcoholic liver disease (ALD). Artichoke (Cynara scolymus L.) is a healthy food folk medicine with anti-oxidative anti-inflammatory properties. This study aimed to evaluate the preventive effects of ethanolic extract from artichoke against acute alcohol-induced injury mice. Male Institute Cancer Research mice were treated an (0.4, 0.8, 1.6 g/kg body weight) by gavage once daily. Up 40% alcohol (12 mL/kg was...
The rat kidney ablation and infarction (A/I) model of subtotal or 5/6th nephrectomy is the most commonly studied nondiabetic chronic disease (CKD). A/I at 1 wk exhibits reductions in function, as determined by glomerular filtration rate, diminished metabolic efficiency oxygen consumption per sodium transport (QO2/TNa). As renoprotective AMPK activity affected changes cellular stress, we evaluated this system. We show that these early pathophysiological are accompanied a paradoxical decrease...
To explore the composition of ocular microbiome in normal subjects and patients with Meibomian gland dysfunction (MGD).Seventy (140 eyes) were enrolled our study. Signs dry eye evaluated bacterial species conjunctival sac (CS) (MG) secretions then identified by 16S rRNA gene sequencing. Additionally, 17 (34 further to determine differences microbiomes surface deep layers MG using a segmental secretion analysis.The positive isolation rate was markedly higher than CS. The control mild group...
Diabetic nephropathy is the commonest cause of end-stage renal disease. Inordinate kidney growth and glomerular hyperfiltration at very early stages diabetes are putative antecedents to this The only organ that grows larger with onset mellitus, yet there remains confusion about mechanism significance growth. Here we show proximal tubule cells in culture transition senescence response oxidative stress. We further determine temporal expression G(1) phase cell cycle components rat cortex days 4...
The 5/6 th nephrectomy or ablation/infarction (A/I) preparation has been used as a classic model of chronic kidney disease (CKD). We observed increased oxygen consumption (Q O 2 ) and altered renal hemodynamics in the A/I that were normalized after combined angiotensin II (ANG II) blockade. Studies suggest hypoxia inducible factor protective influence A/I. induced hypoxia-inducible (HIF) HIF target proteins by two different methods, cobalt chloride (CoCl dimethyloxalyglycine (DMOG), for...
Laser wakefield acceleration of electrons well beyond 1 GeV and optical guiding ultraintense laser pulses peak powers up to 160 TW over a 4-cm long ablative capillary discharge plasma channel were experimentally demonstrated. Electron beams, with energies 1.8 GeV, generated by using the 130 TW, 55 fs driving pulses. A comparison oxygen-containing acrylic resin (C:O:H = 4:2:7) no polyethylene 1:0:2) measurements suggests that injection electron into is assisted ionization oxygen K-shell electrons.
Abstract Purpose: Bladder cancer is one of the most common urinary malignancies worldwide characterized by a high rate recurrence and no targeted therapy method. stem cells (BCSCs) play crucial role in tumor initiation, metastasis, drug resistance. However, regulatory signaling self-renewal mechanisms BCSCs remain largely unknown. Here, we identified novel signal, KMT1A-GATA3-STAT3 circuit, which promoted tumorigenicity human BCSCs. Experimental Design: In discovery step, bladder non-stem...
Consideration of laser-driven plasma-based electron/positron accelerators with a 2 TeV center-of-mass energy is presented, employing multistaging scheme consisting successive multi-GeV laser wakefield operated at the plasma density range ${10}^{15}--{10}^{18}\text{ }\text{ }{\mathrm{cm}}^{\ensuremath{-}3}$ in quasilinear regime. A total accelerator length determined by an operating and coupling distance allowed for both beam focusing systems. We investigate dynamics synchrotron radiation due...
It is hypothesized that "cancer stem cells" are responsible for the resistance to chemotherapy of cancer cells in ovarian cancers.The objective studies was explore if cell biomarkers could be used predict tumor chemotherapy-resistance serous patients.Expression two putative markers CD133 and nestin, vascular epithelial growth factor (VEGF) epidermal receptor (EGFR) were detected 123 cases advanced specimens by immunohistochemistry.To estimate intra-tumoral microvessel density (MVD), CD34...
Earth's near space is an extreme atmosphere environment with high levels of radiation, low atmospheric pressure and dramatic temperature fluctuations. The region above the flight altitude aircraft but below orbit satellites, which has special Mars-like conditions for investigating survival evolution life. Technical limitations including devices, payloads technologies/methodologies hinder microbiological research in space. In this study, we investigated microbial adaptive strategies using a...