A5088465643- Acute Ischemic Stroke Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Cerebrovascular and Carotid Artery Diseases
- S100 Proteins and Annexins
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Atrial Fibrillation Management and Outcomes
- Traumatic Brain Injury and Neurovascular Disturbances
- Atherosclerosis and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Neurological Disease Mechanisms and Treatments
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Stroke Rehabilitation and Recovery
- Cerebrovascular and genetic disorders
- Kruppel-like factors research
- Immune cells in cancer
- Alzheimer's disease research and treatments
- Intracranial Aneurysms: Treatment and Complications
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Neurological Disorders and Treatments
- RNA regulation and disease
- Cardiovascular Health and Disease Prevention
University of Alberta
2009-2025
Women and Children’s Health Research Institute
2019-2024
University of California, Davis
2014-2024
United Nations Economic and Social Commission for Asia and the Pacific
2021-2024
Deleted Institution
2023
National Institute of Neurological Disorders and Stroke
2023
Centre for Mental Health
2022
University of Calgary
2000-2022
St. Francis Xavier University
2022
Foothills Medical Centre
2022
MicroRNAs (miRNAs) regulate gene expression and have a critical role in many biologic pathologic processes. We hypothesized that miRNA profiles injured brain (hippocampus) would show common as well unique when compared with those of blood. Adult, untouched, control rats were sham surgeries, ischemic strokes, hemorrhage (lysed blood, fresh or thrombin), kainate-induced seizures. Brain whole-blood assessed 24 h later using TaqMan rodent arrays. MicroRNA response different for each condition....
<h3>Importance</h3> The relative rates of detection atrial fibrillation (AF) or flutter from evaluating patients with prolonged electrocardiographic monitoring an external loop recorder implantable after ischemic stroke are unknown. <h3>Objective</h3> To determine, in a recent stroke, whether 12 months detects more occurrences AF compared conventional for 30 days. <h3>Design, Setting, and Participants</h3> Investigator-initiated, open-label, randomized clinical trial conducted at 2...
microRNA (miRNA) are important regulators of gene expression. In patients with ischemic stroke we have previously shown that differences in immune cell expression present. this study sought to determine the miRNA differentially expressed peripheral blood cells acute and thus may regulate expression.miRNA from forty-eight vascular risk factor controls were compared. Differentially determined by microarray qRT-PCR confirmation. The targets pathways associated be regulated identified...
A blood-based biomarker of acute ischemic stroke would be significant value in clinical practice. This study aimed to (1) replicate a larger cohort our previous using gene expression profiling predict stroke; and (2) refine prediction by including control groups relevant stroke.Patients with (n=70, 199 samples) were compared subjects who healthy (n=38), had vascular risk factors (n=52), myocardial infarction (n=17). Whole blood was drawn ≤3 hours, 5 24 hours after onset from subjects. RNA...
Background and Purpose— Although peripheral blood mRNA micro-RNA change after ischemic stroke, any role for long noncoding RNA (lncRNA), which comprise most of the genome have been implicated in various diseases, is unknown. Thus, we hypothesized that lncRNA expression also changes stroke. Methods— was assessed 266 whole-blood samples drawn once per individual from patients with stroke matched vascular risk factor controls. Differential by ANCOVA ( P <0.005; fold change>|1.2|),...
Abstract Objective: The cause of stroke remains unknown or cryptogenic in many patients. We sought to determine whether gene expression signatures blood can distinguish between cardioembolic and large‐vessel causes stroke, these profiles predict etiology the group. Methods: A total 194 samples from 76 acute ischemic patients were analyzed. RNA was isolated run on Affymetrix U133 Plus2.0 microarrays. Genes that determined at 3, 5, 24 hours following onset. Predictors evaluated using...
Aims Epidemiological studies suggest that sex has a role in the pathogenesis of cardioembolic stroke. Since stroke is vascular disease, identifying sexually dimorphic gene expression changes blood leukocytes can inform on sex-specific risk factors, response and outcome biology. We aimed to examine immune following by studying differential peripheral white cells. Methods Results Blood samples from patients with were obtained at ≤3 hours (prior treatment), 5 24 (after treatment) after onset (n...
Importance Age is a leading predictor of poor outcomes after brain injuries like stroke. The extent to which age associated with preexisting burdens changes, visible on neuroimaging but rarely considered in acute decision-making or trials, unknown. Objectives To explore the mediation functional outcome by markers frailty (hereinafter frailty) patients ischemic stroke receiving endovascular thrombectomy (EVT). Design, Setting, and Participants This cohort study was post hoc analysis Safety...
Gene expression studies require appropriate normalization methods. One such method uses stably expressed reference genes. Since suitable genes appear to be unique for each tissue, we have identified an optimal set of the most in human blood that can used normalization.Whole-genome Affymetrix Human 2.0 Plus arrays were examined from 526 samples males and females ages 2 78, including control subjects patients with Tourette syndrome, stroke, migraine, muscular dystrophy, autism. The top 100 a...
The cause of ischemic stroke remains unclear, or cryptogenic, in as many 35% patients with stroke. Not knowing the restricts optimal implementation prevention therapy and limits research. We demonstrate how gene expression profiles blood can be used conjunction a measure infarct location on neuroimaging to predict probable cryptogenic stroke.The was predicted using previously described differentially expressed genes characteristic cardioembolic, arterial, lacunar RNA isolated from peripheral...
Because our recent studies have demonstrated that miR-122 decreased in whole blood of patients and rats following ischemic stroke, we tested whether elevating would improve stroke outcomes rats. Young adult were subjected to a temporary middle cerebral artery occlusion (MCAO) or sham operation. A polyethylene glycol-liposome-based transfection system was used administer mimic after MCAO. Neurological deficits, brain infarction, vessel integrity, adhesion molecule expression target...
The goal of this study was to show that myelin and axons in cortical gray matter are damaged Alzheimer's disease (AD) brain. Superior temporal gyrus AD patients (9 male, 14 female) compared cognitively normal controls (8 male
Objective: Myelin disruption is an important feature of Alzheimer’s disease (AD) that contributes to impairment neuronal circuitry and cognition. In this study we characterize myelin degradation in the brains patients with compared normal aged controls. Methods: from AD (n=13) was matched controls (n=6). examined by immunohistochemistry frontal white matter (WM) for intact basic protein (MBP), degraded MBP, presence lipid PAS staining. The relationship axonal injury also assessed. Results:...
Intracerebral hemorrhage (ICH) has a high morbidity and mortality. The peripheral immune system cross-talk between blood brain have been implicated in the ICH response. Thus, we delineated gene networks associated with human transcriptome. We also compared differentially expressed genes following to prior study of perihematomal tissue. performed whole-transcriptome analysis matched vascular risk factor control subjects (n = 66). Gene co-expression network identified groups co-expressed...
IL-6 (interleukin-6) has important roles in atherosclerosis pathophysiology. To determine if anti-IL-6 therapy warrants evaluation as an adjuvant stroke prevention strategy patients with carotid atherosclerosis, we tested whether circulating levels predict plaque severity, vulnerability, and progression the prospective population-based CHS (Cardiovascular Health Study).Duplex ultrasound was performed at baseline 5 years. Baseline severity scored 0 to based on North American Symptomatic...