A5086659542- Pain Mechanisms and Treatments
- Dermatology and Skin Diseases
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Asthma and respiratory diseases
- Spaceflight effects on biology
- Urticaria and Related Conditions
- Physics of Superconductivity and Magnetism
- Stress Responses and Cortisol
- Superconducting and THz Device Technology
- Magnetic and transport properties of perovskites and related materials
- Neurogenesis and neuroplasticity mechanisms
- Insect Resistance and Genetics
- RNA Research and Splicing
- Silk-based biomaterials and applications
- Photoreceptor and optogenetics research
- Hormonal Regulation and Hypertension
- Spacecraft and Cryogenic Technologies
- Animal Genetics and Reproduction
- Insect Utilization and Effects
- Lymphoma Diagnosis and Treatment
- Memory and Neural Mechanisms
- Ion Channels and Receptors
- Insect and Arachnid Ecology and Behavior
Tohoku University
2009-2024
Kyushu University
2007-2024
Hyogo University
2023-2024
Pharmac
2023
Chiba University
2023
Hyogo Medical University
2020-2022
Takeda (Japan)
2003-2017
Taisho Pharmaceutical (Japan)
2016
Japan Aerospace Exploration Agency
2014
University of Toronto
2014
In cell transplantation therapy for spinal cord injury (SCI), grafted human induced pluripotent stem cell-derived neural stem/progenitor cells (hiPSC-NS/PCs) mainly differentiate into neurons, forming synapses in a process similar to neurodevelopment. the developing nervous system, activity of immature neurons has an important role constructing and maintaining new synapses. Thus, we investigate how enhancing transplanted hiPSC-NS/PCs affects both themselves host tissue. We find that...
Abstract Neuropathic pain is caused by peripheral nerve injury (PNI). One hallmark symptom allodynia (pain normally innocuous stimuli), but its mechanistic underpinning remains elusive. Notably, whether selective stimulation of non-nociceptive primary afferent Aβ fibers indeed evokes neuropathic pain-like sensory and emotional behaviors after PNI unknown, because the lack tools to manipulate fiber function in awake, freely moving animals. In this study, we used a transgenic rat line that...
A cardinal, intractable symptom of neuropathic pain is mechanical allodynia, caused by innocuous stimuli via low-threshold mechanoreceptors such as Aβ fibers. However, the mechanism which fiber-derived signals are converted to remains incompletely understood. Here we identify a subset inhibitory interneurons in spinal dorsal horn (SDH) operated adeno-associated viral vectors incorporating neuropeptide Y promoter (AAV-NpyP+) and show that specific ablation or silencing AAV-NpyP+ SDH...
Abstract Reactive astrocytes play a pivotal role in the pathogenesis of neurological diseases; however, their functional phenotype and downstream molecules by which they modify disease remain unclear. Here, we genetically increase P2Y 1 receptor (P2Y1R) expression, is upregulated reactive several diseases, male mice to explore its function molecule. This astrocyte-specific P2Y1R overexpression causes neuronal hyperexcitability increasing both astrocytic Ca 2+ signals. We identify...
Abstract The anterior cingulate cortex (ACC) responds to noxious and innocuous sensory inputs, integrates them coordinate appropriate behavioral reactions. However, the role of projections ACC neurons subcortical areas their influence on processing are not fully investigated. Here, we identified that projecting contralateral claustrum (ACC →contraCLA ) preferentially respond mechanical stimulation. These responses were enhanced during attending behavior. Optogenetic activation silenced...
During rapid eye movement (REM) sleep, anti-gravity muscle tone and bodily movements are mostly absent, because somatic motoneurons inhibited by descending inhibitory pathways. Recent studies showed that glycine/GABA neurons in the ventromedial medulla (VMM; Gly VMM neurons) play an important role generating atonia during REM sleep (REM-atonia). However, how these REM-atonia-inducing interconnect with other neuronal populations has been unknown. In present study, we first identified a...
Neuropathic pain, an intractable pain symptom that occurs after nerve damage, is caused by the aberrant excitability of spinal dorsal horn (SDH) neurons. Gabapentinoids, most commonly used drugs for neuropathic inhibit calcium-mediated neurotransmitter release binding to α2δ-1, a subunit voltage-gated calcium channels, and alleviate pain. However, exact contribution α2δ-1 expressed in SDH neurons altered synaptic transmission mechanical hypersensitivity following injury not fully understood....
Inhibitory interneurons in the spinal dorsal horn (SDH) are crucial for processing somatosensory information originating periphery. However, effects of acute and selective inactivation GABAergic SDH on pain not fully understood. In this study, we used designer receptors exclusively activated by drugs (DREADD) technology vesicular GABA transporter-Cre (Vgat-Cre) mice to selectively express a modified human muscarinic Gi protein-coupled receptor (hM4Di) Vgat-Cre + fourth lumbar segment. We...
Signal transducer and activator of transcription 3 (STAT3) has been shown to mediate the anti-inflammatory effect IL-10. Activated STAT3 suppresses LPS-induced IL-6, tumor necrosis factor-α IL-12 gene expression in macrophages dendritic cells. However, mechanism Toll-like receptor (TLR) signal suppression by not clarified. In this study, we investigated constitutively activated (STAT3C) on nuclear factor-κB (NF-κB) activation. The forced STAT3C HEK293/TLR4 cells, but neither wild-type nor...
Temporal properties of spike firing in the central nervous system (CNS) are critical for neuronal coding and precision information storage. Chronic pain has been reported to affect cognitive emotional functions, addition trigger long-term plasticity sensory synapses behavioral sensitization. Less is known about possible changes temporal cortical neurons chronic conditions. In present study, we investigated action potential anterior cingulate cortex (ACC) by using both <i>in vivo</i>...
Label-free measurement is essential to understand the metabolism of drug molecules introduced into cells. Raman imaging a powerful method investigate intracellular because it provides in situ label-free observation molecules. In this study, we propose that can be used not only observe distribution but also quantitatively visualize concentration reflecting each organelle single living cell using band extracellular water as an intensity standard. We dissolved poorly water-soluble...
The aim of this study was to investigate the efficacy and safety combination therapy interferon ribavirin for aged patients with chronic hepatitis C.This conducted at Osaka University Hospital institutions participating in Liver Disease Study Group on 329 C receiving (group A, under 60 year old, n=199; group B, 60-64 n=64; C, over 65 old (mean age, 67.8+/-2.2 n=66)). Of 293 who were tested HCV serotype viral loads, 215 had HCV-RNA 1 high loads (1H) other 78 2 or low (non-1H).In per-protocol...
An excitatory neuron subset in the spinal dorsal horn (SDH) that expresses gastrin-releasing peptide receptors (GRPR) is critical for pruriceptive transmission. Here, we show glutamatergic inputs onto GRPR+ neurons are facilitated mouse models of chronic itch. In these models, neuronal pentraxin 2 (NPTX2), an activity-dependent immediate early gene product, upregulated root ganglion (DRG) neurons. Electron microscopy reveals NPTX2 present at presynaptic terminals connected postsynaptic...
We present the mission design of LiteBIRD, a next generation satellite for study B-mode polarization and inflation from cosmic microwave background radiation (CMB) detection. The science goal LiteBIRD is to measure CMB with sensitivity δr = 0:001, this allows testing major single-field slow-roll models experimentally. instrumental purely driven achieve goal. At earlier stage design, several key specifications, e.g. observing band, optical system, scan strategy, orbit, need be defined in...
Abstract Itch is defined as an unpleasant sensation that provokes a desire to scratch. Our understanding of neuronal circuits for itch information transmission and processing in the spinal dorsal horn (SDH) has progressively advanced following identification SDH neuron subsets are crucial scratching behavior models itch. However, little known about control acute chronic by descending signals from brain SDH. In this study, using genetic approaches enable cell-type circuit-specific functional...
It is well known that acute exposure to physical stress produces a transient antinociceptive effect (called stress-induced analgesia [SIA]). One proposed mechanism for SIA involves noradrenaline (NA) in the central nervous system. NA has been reported activate inhibitory neurons spinal dorsal horn (SDH), but its vivo role remains unknown. In this study, we found an on noxious heat after restraint was impaired mice with conditional knockout of α1A-adrenaline receptors (α1A-ARs)...
Mechanical allodynia (pain produced by innocuous stimuli such as touch) is the main symptom of neuropathic pain. Its underlying mechanism remains to be elucidated, but peripheral nerve injury (PNI)-induced malfunction neuronal circuits in central nervous system, including spinal dorsal horn (SDH), thought involved touch-pain conversion. Here, we found that intra-SDH injection adeno-associated viral vectors a prodynorphin promoter (AAV-PdynP) captured subset neurons were mainly located...
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