A5100392278- Microtubule and mitosis dynamics
- Heat shock proteins research
- Supramolecular Chemistry and Complexes
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Toxin Mechanisms and Immunotoxins
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Protein Structure and Dynamics
- Biological Research and Disease Studies
- Nuclear Structure and Function
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Enzyme Structure and Function
- thermodynamics and calorimetric analyses
- Endoplasmic Reticulum Stress and Disease
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Advanced Control Systems Design
- Gene Regulatory Network Analysis
- Monoclonal and Polyclonal Antibodies Research
Harbin Medical University
2020-2025
Third Affiliated Hospital of Harbin Medical University
2025
Shanghai Jiao Tong University
2003-2025
Shanghai Mental Health Center
2025
Peking Union Medical College Hospital
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Second Affiliated Hospital of Zhejiang University
2018-2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2024
Hangzhou Medical College
2024
Zhejiang Provincial People's Hospital
2024
Significance FAT10, a ubiquitin-like modifier, is an oncogene that interacts with mitotic arrest-deficient 2 (MAD2) and confers cellular malignancy. Here we identified the MAD2-binding residues of FAT10 determined first solution structure, to our knowledge, domain. Importantly, demonstrated proof-of-mechanism for novel specific drug-targeting strategy entails inhibition pathological activity therapeutic target but not its reported physiological function, thus minimizing undesirable side...
Mps1 is a protein kinase that plays essential roles in spindle checkpoint signaling. Unattached kinetochores or lack of tension triggers recruitment several key proteins to the kinetochore, which delays anaphase onset until proper attachment reestablished. acts upstream signaling cascade, and kinetochore targeting required for subsequent Mad1 Mad2 kinetochore. The mechanisms govern other upon activation are incompletely understood. Here, we demonstrate phosphorylation at T12 S15 requires its...
The purpose of this research was to study the roles chloride intracellular channel protein 1 (CLIC1) and heat shock 27 (HSP27) in clinical pathology lung adenocarcinoma explore whether expression CLIC1 HSP27 can be used as independent factors for prediction recurrence prognosis after radical resection adenocarcinoma. One hundred three paraffin sections tissues were collected, detected these tumors using immunohistochemistry. correlation two proteins with clinicopathological parameters...
Abstract Ambient air temperature is a key factor affecting human health. Female reproductive disorders are representative health risk events under low temperature. However, the mechanism involving in cold-induced female remains largely unknown. mice were intermittently exposed to cold conditions (4 °C) address of on system. Primary granulosa cells (GCs) prepared and cultured (35 or β3-adrenoreceptor agonist, isoproterenol, mimic condition exposure. Western-blot, RT-PCR, co-IP, ELISA,...
The existence of both right-half-plane poles and time delay makes it difficult to obtain a higher control quality. Recently, modified Smith predictor for unstable processes with was presented good performance obtained. In this paper, the is discussed. A structure presented. new one not only simple analysis design but also avoids improper element. Instead introduction an inner loop, direct procedure given analytical formulas controllers are provided. domain trajectory regulatory responses...
The Schizosaccharomyces pombe ran1/pat1 gene regulates the transition between mitosis and meiosis. Inactivation of Ran1 (Pat1) kinase is necessary sufficient for cells to exit cell cycle undergo yeast two-hybrid interaction trap was used identify protein partners Ran1/Pat1. Here we report identification one these, Cpc2. Cpc2 encodes a homologue RACK1, WD with homology beta subunit heterotrimeric G proteins. RACK1 highly conserved protein, although its function remains undefined. In mammalian...
Abstract Mps1 is one of the several essential kinases whose activation required for robust mitotic spindle checkpoint signalling. The activity tightly regulated and increases dramatically during mitosis or in response to damage. To understand molecular mechanism underlying regulation, we determined crystal structure kinase domain Mps1. 2.7‐Å‐resolution shows that adopts a unique inactive conformation. Intramolecular interactions between key Glu residue αC helix N‐terminal lobe backbone...
Abstract Chromosome instability (CIN) is a common contributor driving the formation and progression of anaplastic thyroid cancer (ATC), but its mechanism remains unclear. The BUB1 mitotic checkpoint serine/threonine kinase (BUB1) responsible for alignment chromosomes, which has not been thoroughly studied in ATC. Our research demonstrated that was remarkably upregulated closely related to worse progression‐free survival. Knockdown attenuated cell viability, invasion, migration induced cycle...
Exosomes can be used to mediate the delivery of nucleic acids such as microRNA‑125b‑5p (miR‑125b‑5p), a tumor‑suppressor in certain types cancer, into tumor cells. The present study investigated use bone mesenchymal stem cells‑derived exosome (BMSCs‑Exo) miR‑125b‑5p ovarian cancer (OC). BMSCs were transfected with mimic, from which exosomes termed Exo‑miR‑125b‑5p mimic extracted. expression levels OC tissue samples, BMSCs, and SKOV3 cells quantified using reverse transcription‑quantitative...
Juvenile systemic sclerosis (jSSc) can lead to permanent and irreversible anatomical or physiological dysfunction. The Scleroderma Clinical Trials Consortium-Damage Index (SCTC-DI), which has been employed validated in adult patients, quantify organ damage predict mortality morbidity. However, its application paediatric patients remains unexplored. data, laboratory results, prognostic information were collected for with jSSc at Peking Union Medical College Hospital (PUMCH) from January 2012...
Aim: To investigate the role and mechanisms of AC245100.4 in prostate cancer. Materials & methods: The expression location were examined using real-time PCR situ hybridization. Cell Counting Kit-8, clone formation, flow cytometry vivo assays conducted to determine AC245100.4. RNA antisense purification with mass spectrometry immunoprecipitation performed identify proteins that bind Western blotting was quantify protein. Results:AC245100.4 upregulated cancer mainly located cytoplasm....
Background Nuclear morphogenesis is one of the most fundamental cellular transformations taking place during spermatogenesis. In rodents, a microtubule-based perinuclear structure, manchette, and C-terminal kinesin motor KIFC1 are believed to play crucial roles in this process. Spermatogenesis Octopus tankahkeei good model system explore whether evolution has created cephalopod prototype mammalian manchette-based KIFC1-dependent sperm nuclear shaping machinery. Methodology/Principal Findings...
Sister chromatid cohesion plays a key role in ensuring precise chromosome segregation during mitosis, which is mediated by the multisubunit cohesin complex. However, molecular regulation of subunits stability remains unclear. Here, we show that NudCL2 (NudC-like protein 2) essential for regulating Hsp90 ATPase activity mammalian cells. Depletion induces mitotic defects and premature sister separation destabilizes interact with NudCL2. Similar are also observed upon inhibition activity....
Kinesin superfamily protein 2A (KIF2A), an M‑type nonmotile microtubule depolymerase, has received attention for its role in carcinogenesis and prognostic value several types of cancer. In this study, we evaluated the expression KIF2A potential robustness to predict clinical outcomes colorectal cancer (CRC) patients. The messenger RNA (mRNA) was determined 20 pairs cancerous adjacent nontumor tissues by real-time polymerase chain reaction. immunohistochemistry performed on tissue microarray...
Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock 90 (Hsp90), modulates cell by stabilizing both myosin-9 and lissencephaly 1 (LIS1). Either knockdown or knockout NudCL2 significantly increases single-cell migration, but has no significant effect on collective migration. Immunoprecipitation-mass spectrometry western blotting analyses reveal binds to...
Abstract Targeting the mitotic motor kinesin spindle protein (KSP) is a new strategy for cancer therapy. We have examined molecular events induced by KSP inhibition and explored possible mechanisms of resistance sensitization tumor cells to inhibitors. found that cell death primarily via activation mitochondrial pathway. In HeLa cells, small-molecule inhibitor monastrol resulted in arrest rapid caspase activation. BclXL phosphorylation loss membrane potential was detected before significant...
The upregulation of both HSP70 and HSP90 frequently compromises the effects thermotherapy. co-inhibition HSP70/HSP90 may be preferable to enhance thermotherapy on nasopharyngeal carcinoma cells. changes were detected after in human cancer cell HNE1. 17-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) quercetin used inhibit activity HSP70. enhanced evaluated vitro vivo. Both upregulated promptly HNE1 Single inhibition resulted overexpression delayed descent HSP90. with plus 17-DMAG...
1. Hypoxia-induced cardiomyocyte apoptosis contributes significantly to cardiac dysfunction following trauma, shock and burn injury. There is evidence that heat protein (HSP) 90 anti-apoptotic in cardiomyocytes subjected a variety of apoptotic stimuli. Because HSP90 acts as an upstream regulator the serine/threonine kinase Akt survival pathway during cellular stress, we hypothesized exerts cardioprotective effect via phosphatidylinositol 3-kinase (PI3-K)/Akt pathway. 2. Neonatal rat were...