A5091090247- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- CRISPR and Genetic Engineering
- EEG and Brain-Computer Interfaces
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Visual perception and processing mechanisms
- Nicotinic Acetylcholine Receptors Study
- Cardiac electrophysiology and arrhythmias
- Virus-based gene therapy research
- S100 Proteins and Annexins
- Cellular Automata and Applications
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
Norwegian University of Science and Technology
2014-2025
University of Oxford
2024
Kavli Institute for Systems Neuroscience
2013-2023
University of Oregon
2011-2023
The University of Texas at Austin
2018
Stony Brook Medicine
2013
Columbia University
1998-2004
New York State Psychiatric Institute
1998-2004
Howard Hughes Medical Institute
1998-2004
McGill University
1998
Hippocampal pyramidal cells are called place because each cell tends to fire only when the animal is in a particular part of environment—the cell's firing field. Acute pharmacological blockade N -methyl- d -aspartate (NMDA) glutamate receptors was used investigate how NMDA-based synaptic plasticity participates formation and maintenance fields. The results suggest that short-term stability fields new environment involve independent NMDA receptor activation. By contrast, long-term...
Adding Artificial Associations In the mammalian cortex, there is significant spontaneous neural activity that internally generated, rather than arising from sensory inputs, and this influences processing of natural stimuli. What role does generated play in forming accessing new memory representations? Using transgenic mice combined with controlled artificial activation widely distributed neuronal ensembles associated specific contexts, Garner et al. (p. 1513 ; see Perspective by Morris...
The family of mammalian genes related to the Drosophila Shaker gene, consisting four subfamilies, is thought encode subunits tetrameric voltage-gated K+ channels. There compelling evidence that same subfamily, but not different form heteromultimeric channels in vitro, and thus, each gene subfamily postulated components an independent channel system. In order identify cells with native containing one these subfamilies (Shaw-related or ShIII), cellular distribution ShIII transcripts was...
1. Xenopus oocytes injected with rat brain mRNA express a transient K+ current similar to the A that activates transiently near threshold for Na+ action potential generation (ISA) seen in somatic recordings from neurons. We used hybrid arrest antisense oligonucleotides investigate which of cloned channel proteins might be components channels responsible ISA expressed mRNA. An oligonucleotide complementary sequence common all known mammalian Shal-related mRNAs [KV4.1, KV4.2, and KV4.3 (the...
A key question in the analysis of hippocampal memory relates to how attention modulates encoding and long-term retrieval spatial nonspatial representations this region. To address question, we recorded from single cells over a period 5 days CA1 region dorsal hippocampus while mice acquired one two goal-oriented tasks. These tasks required animals find hidden food reward by attending either visuospatial environment or particular odor presented shifting locations. Attention increased stability...
Layer II of the medial entorhinal cortex (MEC) contains two principal cell types: pyramidal cells and stellate cells. Accumulating evidence suggests that these types have distinct molecular profiles, physiological properties, connectivity. The observations hint at a fundamental functional difference between populations but conclusions been mixed. Here, we used tTA-based transgenic mouse line to drive expression ArchT, an optogenetic silencer, specifically in We were able optogenetically...
Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CLCX), this has yet be fully accomplished. The CLCX is generally considered comprise two major subdivisions, (CL) and dorsal endopiriform nucleus (DEn), regional boundaries these areas debated. To address this, we conducted multifaceted analysis of fiber- cytoarchitecture, genetic marker expression, connectivity using mice both sexes, create comprehensive...
G-protein-gated inward rectifier potassium (GIRK) channels are coupled to numerous neurotransmitter receptors in the brain and can play important roles modulating neuronal function, depending on their localization a given neuron. Site-directed antibodies extreme C terminus of GIRK1 (or KGA1), recently cloned component GIRK channels, have been used determine relative expression levels distribution protein different regions rat by immunoblot immunohistochemical techniques. We report that is...
Damage to the hippocampal formation results in a profound temporally graded retrograde amnesia, implying that it is necessary for memory acquisition but not its long-term storage. It therefore thought memories are transferred from hippocampus cortex storage process called systems consolidation (Dudai and Morris, 2000). Where this occurs remains an open question. Recent work (Frankland et al., 2005; Vetere 2011) suggests anterior cingulate (ACC) as likely candidate area, there little direct...
The enormous potential of modern molecular neuroanatomical tools lies in their ability to determine the precise connectivity neuronal cell types comprising innate circuitry brain. We used transgenically targeted viral tracing identify monosynaptic inputs projection neurons layer II medial entorhinal cortex (MEC-LII) mice. These are not only major hippocampus, structure most clearly implicated learning and memory, they also “grid cells.” Here we address question what kinds specifically...
The anterior cingulate cortex (ACC) is a component of the limbic system implicated in wide variety functions spanning motor and sensory information processing, memory, attention, novelty detection, comparisons expectation versus outcome. It remains unclear how much this functional diversity stems from differences methodology or interpretation truly reflecting range processes which ACC involved. In present study, neuronal activity was examined freely behaving mice (C57BL6/J) under conditions...
Although a variety of remarkable molecular tools for studying neural circuits have recently been developed, the ability to deploy them in particular neuronal subtypes is limited by fact that native promoters are almost never specific enough. We showed one can generate transgenic mice with anatomical specificity surpassing combining enhancers uniquely active brain regions heterologous minimal promoter, an approach we call EDGE (Enhancer-Driven Gene Expression). Here extend this strategy...
Understanding how neural circuits work requires a detailed knowledge of cellular-level connectivity. Our current understanding circuitry is limited by the constraints existing tools for transsynaptic tracing. Some most intractable problems are lack cellular specificity uptake, transport across multiple synaptic steps conflating direct and indirect inputs, poor labeling minor inputs. We used novel combination transgenic mouse technology recently developed tracing system based on rabies virus...
Fan cells in layer II of the lateral entorhinal cortex (LEC) form a main component projection to dentate gyrus, CA3 and CA2 hippocampal formation. This has counterpart originating from stellate medial (MEC). Available evidence suggests that two pathways carry different information, exemplified by difference spatial tuning LEC MEC. The grid cell, prominent position-modulated cell type present MEC, been postulated derive its characteristic hexagonal firing pattern dominant disynaptic...
The claustrum is thought to be one of the most highly interconnected forebrain structures but its organizing principles have yet fully explored at level single neurons. Here, we investigated identity, connectivity, and activity identified neurons understand how structure's unique convergence input divergence output support binding information streams. We found that in communicate with each other across efferent projection-defined modules which were differentially innervated by sensory...
The hippocampus is critical for formation of spatial memories. Hippocampal pyramidal neurons in freely behaving animals exhibit spatially selective firing patterns, which taken together form an internal representation the environment. This thought to contribute hippocampal memory system. Behavioral long-term memories differ from short-term requiring synthesis new proteins. Does development also require proteins? We found that blocking protein brain mice by 95% does not affect stability newly...
The finding that some K+ channel mRNAs are restricted to certain populations of neurons in the CNS suggests there channels tailored neuronal circuits. One such example transcripts from KV3.2 gene, majority which expressed thalamic relay neurons. To gain insights into specific roles subunits, site antibodies were raised determine their localization Immunohistochemical and focal lesioning studies demonstrate proteins localized terminal fields thalamocortical projections. It is also shown vitro...
Mice lacking the serotonin 1A receptor (5-HT R) show increased levels of anxiety-related behavior across multiple tests and background strains. Tissue-specific rescue experiments, lesion studies, neurophysiological findings all point toward hippocampus as a potential mediator phenotype. Serotonin, acting through 5-HT Rs, can suppress hippocampal theta-frequency oscillations, suggesting that theta oscillations might be in knock-outs. To test this hypothesis, local field recordings were...