A5017066267- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Radiomics and Machine Learning in Medical Imaging
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Genomics and Phylogenetic Studies
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Plant Stress Responses and Tolerance
- NF-κB Signaling Pathways
- Atherosclerosis and Cardiovascular Diseases
- Plant Molecular Biology Research
- Pancreatic and Hepatic Oncology Research
- Plasmonic and Surface Plasmon Research
- interferon and immune responses
- Molecular Biology Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- IL-33, ST2, and ILC Pathways
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
Guizhou Provincial People's Hospital
2024-2025
Shanghai University of Medicine and Health Sciences
2025
Model Animal Research Center
2017-2024
University of California, San Diego
2017-2024
Shanghai Skin Disease Hospital
2023-2024
Tongji University
2023-2024
Chongqing Medical University
2024
Zunyi Medical University
2022-2024
Nanjing Drum Tower Hospital
2018-2024
University of Nebraska–Lincoln
2017-2022
Salinity is a major factor limiting crop productivity. Rice (Oryza sativa), staple for the majority of world, highly sensitive to salinity stress. To discover novel sources genetic variation salt tolerance-related traits in rice, we screened 390 diverse accessions under 14 days moderate (9 dS·m-1) salinity. In this study, shoot growth responses levels were independent tissue Na+ content. A significant difference root content was observed between subpopulations with indica displaying higher...
Stress memory refers to the observation that an initial, sub-lethal stress alters plants' responses subsequent stresses. Previous transcriptome analyses of maize seedlings exposed a repeated dehydration has revealed existence transcriptional in Zea mays. Whether drought-related physiological also display and how translates into are fundamental questions still unanswered. Using systems-biology approach we investigate whether/how transcription established genome-wide analysis Z. mays correlate...
Early vigor is an important trait for many rice ( L.)-growing environments. However, genetic characterization and improvement early hindered by the temporal nature of strong genotype × environment effects. We explored architecture shoot growth dynamics during active tillering stages applying a functional modeling genomewide association (GWAS) mapping approach on diversity panel ∼360 accessions. Multiple loci with small effects trajectory were identified, indicating complex polygenic...
SPEG (Striated muscle preferentially expressed protein kinase) has 2 kinase-domains and is critical for cardiac development function. However, it not clear how these function to maintain performance.To determine the molecular functions of SPEG.A proteomics approach identified SERCA2a (sarcoplasmic/endoplasmic reticulum calcium ATPase 2a) as a interacting with second kinase-domain but first SPEG. Furthermore, could phosphorylate Thr484 on SERCA2a, promote its oligomerization increase reuptake...
(1) Background: Radiomics use high-throughput mining of medical imaging data to extract unique information and predict tumor behavior. Currently available clinical prediction models poorly treatment outcomes in pancreatic adenocarcinoma. Therefore, we used radiomic features primary tumors develop outcome compared them traditional models. (2) Methods: We extracted analyzed from pre-radiation contrast-enhanced CTs 74 cancer patients undergoing stereotactic body radiotherapy. A panel over 800...
Abstract Diabetic cardiomyopathy is a progressive disease in diabetic patients, and myocardial insulin resistance contributes to its pathogenesis through incompletely-defined mechanisms. Striated muscle preferentially expressed protein kinase (SPEG) has two kinase-domains critical cardiac regulator. Here we show that SPEG phosphorylated on Ser 2461 /Ser 2462 /Thr 2463 by B (PKB) response insulin. PKB-mediated phosphorylation of activates second kinase-domain, which turn phosphorylates...
Summary Pre‐mRNA splicing is an essential step for the regulation of gene expression. In order to specifically capture variants in plants genome‐wide association studies (GWAS), we developed a software tool quantify and visualise Variations Splicing Population (V SP). V SP can from short‐read RNA‐seq datasets discover genotype‐specific (GSS) events, which be used prioritise causal pre‐mRNA events GWAS. We applied our method dataset with 328 samples 82 genotypes rice diversity panel exposed...
Radiomic analysis has recently demonstrated versatile uses in improving diagnostic and prognostic prediction accuracy for lung cancer. However, since tumors are subject to substantial motion due respiration, the stability of radiomic features over respiratory cycle patient needs be investigated better evaluate robustness inter-patient feature variability clinical applications, its impact such applications assessed. A full panel 841 features, including tumor intensity, shape, texture, wavelet...
Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs their derivatives remains very challenging. Here, we report a novel physiologically relevant 3D culture system (called AlgTube cell system) hPSC expansion differentiation. With this system, processed into cultured microscale alginate hydrogel tubes that suspended medium vessel. The protect from hydrodynamic stresses...
Neural stem cells derived from human pluripotent (hPSC-NSCs) are of great value for modeling diseases, developing drugs, and treating neurological disorders. However, manufacturing high-quantity -quality hPSC-NSCs, especially clinical applications, remains a challenge. Here, we report chemically defined, high-yield, scalable bioprocess hPSC-NSCs. hPSCs expanded differentiated into NSCs in microscale tubes made with alginate hydrogels. The used to isolate the hydrodynamic stresses culture...
This study aimed to explore the role of CHAF1A in hepatocellular carcinoma (HCC) progression, focusing on its expression, co-expression genes, genomic alterations, promoter methylation, clinical relevance, prognostic value, and immune associations. mRNA expression was analyzed using UALCAN. Co-expression genes functions were explored via LinkedOmics. Genomic alterations assessed cBioPortal. Promoter methylation correlations examined GEO datasets. Prognostic significance evaluated...
RORγt controls the differentiation of TH17 cells, which are mediators autoimmune conditions such as experimental encephalomyelitis (EAE). also regulates thymocyte development and lymph node genesis. Here we show that function is regulated by its sumoylation. Loss Sumo3, but not Sumo1, dampens delays progression thymic CD8+ immature single-positive cells (ISPs). SUMO3-modified E3 ligase PIAS4 at lysine 31 (K31), mutation K31 to arginine in mice prevents sumoylation, leading impaired...
Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity hPSC-VSMCs with current culture technologies remains very challenging. Here, we report scalable method manufacturing in alginate hydrogel microtubes (i.e., AlgTubes), which protect from hydrodynamic stresses limit mass to <400 μm ensure efficient transport. The tubes provide friendly...
Further study on steam-treated notoginseng, the roots of Panax notoginseng (Burk.) F.H. Chen (Araliaceae), which is a famous traditional Chinese medicine that used both in raw and treated forms for long time, led to isolation new dammarane-type saponin, namely notoginsenoside ST-4. Its structure was elucidated be 3β,12β,20(S)-tri-hydroxydammar-24-ene-3-O-β-d-xylopyranosyl-(1 → 2)-β-d-glucopyranosyl-(1 2)-β-d-glu-copyranoside, based detailed analyses 1D 2D NMR spectral data acidic hydrolysis....
Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity -quality ECs remains very challenging. Here, we report a method the scalable manufacturing from human pluripotent stem (hPSCs). hPSCs expanded differentiated into in 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects hydrodynamic stresses culture vessel prevents excessive agglomeration, leading to high-culture efficiency including high-viability (>90%),...
Abstract Background Cultivated rice consists of two subspecies, Indica and Japonica , that exhibit well-characterized differences at the morphological genetic levels. However, between these subspecies transcriptome level remains largely unexamined. Here, we provide a comprehensive characterization divergence cis-regulatory variation within using data from 91 accessions diversity panel (RDP1). Results The transcriptomes are highly divergent. have significantly lower expression relative to...
Skeletal muscle absorbs long-chain fatty acids (LCFAs) that are either oxidized in mitochondria or temporarily stored as triglycerides lipid droplets (LDs). So far, it is still not fully understood how uptake and storage regulated whether these important for whole-body homeostasis. Here we show the small GTPase Rab8a regulates skeletal muscle. Muscle-specific deletion caused hyperlipidemia exacerbated hepatosteatosis induced by a high-fat diet. Mechanistically, deficiency decreased LCFA...