A5086642072- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Cancer-related molecular mechanisms research
- Diet, Metabolism, and Disease
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Dietary Effects on Health
- Lipid Membrane Structure and Behavior
- Epigenetics and DNA Methylation
- Fluid Dynamics and Turbulent Flows
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- RNA regulation and disease
- Nanopore and Nanochannel Transport Studies
- Field-Flow Fractionation Techniques
- Cellular transport and secretion
- Adenosine and Purinergic Signaling
- Neural dynamics and brain function
Huazhong University of Science and Technology
2017-2024
Guangzhou Experimental Station
2024
Abstract Intratumoral heterogeneity, including epithelial–mesenchymal transition (EMT), is one major cause of therapeutic resistance. The induction ferroptosis, an iron‐dependent death, has the potential in overcoming this resistance to traditional treatment modalities. However, roles distinct EMT phenotypes ferroptosis remain enigma. This study reports that 3D soft fibrin microenvironment confers colorectal cancer (CRC) cells hybrid phenotype and high level ferroptosis. activation histone...
Extracellular vesicles (EVs) have emerged as a promising tool for clinical liquid biopsy. However, the identification of EVs derived from blood samples is hindered by presence abundant plasma proteins, which impairs downstream biochemical analysis EV-associated proteins and nucleic acids. Here, we employed optimized asymmetric flow field-flow fractionation (AF4) combined with density cushion ultracentrifugation (UC) to obtain high-purity intact very low lipoprotein contamination human serum....
The conserved Hippo pathway regulates organ size, tissue homeostasis, and tumorigenesis. Interferon regulatory factor 2 binding protein (IRF2BP2) was originally identified as a transcriptional corepressor. However, the association between IRF2BP2 remains largely unknown. In addition, biological function regulation mechanism of in liver cancer are poorly understood.In this study, we uncovered clinical significance suppressing hepatocellular carcinogenesis. We showed that IRF2BP2, direct...
Numerous studies have highlighted the role of clock genes in diabetes disease and pancreatic β cell functions. However, whether rhythmic long non-coding RNAs involve this process is unknown.
The kinetochore is a proteinaceous complex that essential for proper chromosome segregation. As core member of the inner kinetochore, defects each subunit in CENP-H/I/K cause dysfunction leads to mis-segregation and cell death. However, how assembles promotes function are poorly understood. We here determined crystal structures CENP-I N-terminus alone from Chaetomium thermophilum its with CENP-H/K Thielavia terrestris, verified identified interactions. biochemical analyses show CENP-H CENP-K...
The tumor suppressor p53 is critical for the maintenance of genome stability and protection against malignant transformation, its homeostasis usually regulated by ubiquitination. MDM2 a major E3 ligase ubiquitination, activity enhanced TRIM28. TRIM28 also independently ubiquitinates as an activated MAGE-C2. Moreover, MAGE-C2 highly expressed in various cancers, but detailed mechanisms involved MDM2/TRIM28-mediated ubiquitination remain unknown. Here, we found that directly interacts with...
The enrichment of histone H3 variant CENP-A is the epigenetic mark centromere and initiates assembly kinetochore at centromere. a multi-subunit complex that ensures accurate attachment microtubule faithful segregation sister chromatids during mitosis. As subunit kinetochore, CENP-I localization also depends on CENP-A. However, whether how regulates deposition identity remains unclear. Here, we identified directly interacts with centromeric DNA preferentially recognizes AT-rich elements via...
Abstract The kinetochore is essential for the accurate segregation of sister chromosome in eukaryote cell. Among subunits, five proteins CENP‐O/P/U/Q/R form a stable complex, referred to as CENP‐O class, and are required proper function. Although function structure yeast COMA complex (CENP‐O/P/U/Q homologs) have been revealed extensively, assembly mechanism detail interactions among human class significantly different remain largely unclear. Here, we identified fragment (residues 241‐360)...
Synaptic vesicles (SVs) store and release neurotransmitters at chemical synapses. Precise regulation of SV trafficking, exocytosis endocytosis is crucial for neural transmission. Biochemical characterization SVs, which essential research into neurotransmitter uptake release, requires effective in vitro isolation methods. Here, we describe an improved simple purification protocol isolating SVs from mouse brain within 6 h, achieving a yield approximately 0.4 mg per single brain. The use...
Abstract In mitosis, the accurate segregation of sister chromosomes relies on kinetochore, a multiple subunits complex assembled centromere each chromosome. As core component inner CENP‐I plays important functions to mediate kinetochore assembly and supports faithful chromosome segregation. The structures N‐terminus C‐terminus homologs in with CENP‐H/K have been reported, respectively. Unfortunately, intramolecular interactions are poorly understood, how interacts CENP‐M remains unknown....
The human Ska complex (Ska) localizing to both spindle microtubules and kinetochores is essential for proper chromosome segregation during mitosis. Although several mechanisms have been proposed explain how recruited kinetochores, it still not fully understood. By analyzing Ska3 phosphorylation, we identified six critical Cdk1 sites, including the previously Thr358 Thr360. Mutations of these sites phospho-deficient alanine (6A) in cells completely abolished localization functions...
ABSTRACT The human Ska ( s pindle and k inetochore a ssociated) complex is essential for proper kinetochore-microtubule interactions. Its loading onto the kinetochore Ndc80 (Ndc80C) plays critical role in such function; however, key determinant that docks Ndc80C still remain elusive. Here, we address this question using biochemical reconstitution followed by functional analysis. We identified six Cdk1 sites Ska3 distributed three conserved regions. In vitro , phosphorylation on enhanced WT,...
Abstract As an output effecter of Hippo signaling pathway, the transcription factor TEAD and co-activator YAP play crucial functions in promoting cell proliferation organ size. The tumor suppressor NF2 has been shown to activate LATS1/2 kinases interplay with pathway suppress YAP-TEAD complex. But, whether how could directly regulate remains unknown. We identified a direct link physical interaction between TEAD4. interacted TEAD4 through its FERM domain C-terminal tail, decreased protein...