A5057384200- Greenhouse Technology and Climate Control
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Leaf Properties and Growth Measurement
- Plant Water Relations and Carbon Dynamics
- Smart Agriculture and AI
- Light effects on plants
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Remote Sensing in Agriculture
- Postharvest Quality and Shelf Life Management
- Spectroscopy and Chemometric Analyses
- Plant responses to elevated CO2
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Water Quality Monitoring and Analysis
- Advanced Chemical Sensor Technologies
- Horticultural and Viticultural Research
- Coffee research and impacts
- Innovations in Aquaponics and Hydroponics Systems
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
Ehime University
2021-2024
University of Tsukuba
2020-2022
The University of Tokyo
2012-2021
Bioengineering Center
2017
National Center for Genetic Engineering and Biotechnology
2017
John Wiley & Sons (United States)
2017
The use of detached leaves instead whole plants provides an alternative means for recombinant protein production based on Agrobacterium tumefaciens-mediated transient gene overexpression. However, the process high-level in has not yet been established. In this study, we focused leaf handling and maintenance conditions immediately after infiltration with suspension (agroinfiltration) to improve expression Nicotiana benthamiana leaves. We demonstrated that residual water bacterial had...
Predicting the yield of horticultural crops is crucial to meet expectations retailers and consumers. In this study, we developed random forests (RF) based on measured amounts whole-plant photosynthesis transpiration predict cherry tomato fruit yields in a commercial greenhouse Japan. Whole-plant daily net (Photo) (Trans) were by using real-time monitoring system. Variables environmental conditions (Env), including solar irradiation, air temperature, atmospheric water vapor deficit, also...
Viral vector-mediated transient expression enables rapid biopharmaceutical protein production using whole plants. In recent years, plant growth conditions have received close attention as factors influencing the content of recombinant protein. particular, because nitrogen is an important resource used in synthesis, supplied to root zone may influence accumulation proteins. We tested whether fertilization Nicotiana benthamiana with high-nitrate nutrient solution during prior...
Agrobacterium-mediated transient expression systems enable plants to rapidly produce a wide range of recombinant proteins. To achieve economically feasible upstream production and downstream processing, it is beneficial obtain high levels two yield-related quantities production: protein content per fresh mass harvested biomass (g gFM-1 ) productivity unit area-time m-2 /month). Here, we report that the density Nicotiana benthamiana during had significant impacts on hemagglutinin (HA). The...
Skilled farmers generally evaluate the growth of a crop by observing crop's appearance. However, with increasing digitalization agricultural practices, numerical index representing status is strongly needed. For this purpose, speaking plant approach (SPA), sophisticated strategy for environmental control in greenhouses, has attracted lot attention. The first and most important step SPA to obtain physiological information from then judge whether healthy. daily measurement therefore SPA,...
We evaluated averaging techniques in data processing for the estimation of canopy net photosynthetic rates (Pn) two cherry tomato plants using a multiple linear regression analysis with variables aerial environmental factors. Whole Pn and factors were measured high time resolution 5-minute interval under commercial greenhouse by novel photosynthesis chamber. processed moving average (MA) simple (SA) several frames (30-minute, 1-hour, 2-hour). The was expressed as general function PAR...
The objective of this study was to develop an empirical model for whole-plant net photosynthetic rate (Pn, μmol s-1 per plant) as a function four essential environmental factors: photosynthetically active radiation (PAR) above the canopy (I, W m-2), air temperature (T, ℃), CO2 concentration (C, mol-1), and vapor pressure deficit (VPD, kPa) by using dataset Pn corresponding factors. To prepare dataset, we monitored photosynthesis mature cherry tomato plants grown in commercial greenhouse 15...
We investigated effects of dim light irradiation (about 11 μmol m−2 s−1 photon flux density) the root zone on growth and development leaf lettuce (Lactuca sativa ‘Okayama saradana’) plants under nutrient film technique (NFT) hydroponics with monochromatic emitting diodes (LEDs) five types respective peak wavelengths (and colors) 405 nm (violet), 465 (blue), 525 (green), 660 (red), 735 (far-red). Shoot fresh weight specific length in treatments violet, blue, far-red LEDs were significantly...
We developed an open-chamber system to monitor photosynthesis and transpiration of whole plants with high time resolution. The may be promising for environmental controls based on plant stress diagnoses; however, the conventional method used calculate net CO2 exchange rate (NCER) should improved avoid overestimation underestimation NCER under greenhouse conditions. To NCER, uses difference in concentration between inlet outlet air system. concentrations are alternately recorded by a single...
A real-time monitoring system was developed and applied to monitor the time course of photosynthesis transpiration in fully-grown tomato plants a semi-commercial greenhouse. The based on an open chamber method which ventilator airflow rate is important parameter affecting environmental factors physiological response enclosed inside chamber. So, we assumed that effects this these responses should be evaluated for agricultural production site. In study, investigated differences whole-tomato...