A5100351533- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Renal and related cancers
- Olfactory and Sensory Function Studies
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
- biodegradable polymer synthesis and properties
- Protist diversity and phylogeny
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Advanced Chemical Sensor Technologies
- Cancer Research and Treatments
- Photosynthetic Processes and Mechanisms
- Biochemical Analysis and Sensing Techniques
- DNA and Nucleic Acid Chemistry
- Biocrusts and Microbial Ecology
- Recycling and utilization of industrial and municipal waste in materials production
- Rare-earth and actinide compounds
- Proteoglycans and glycosaminoglycans research
Tsinghua University
2001-2025
Center for Life Sciences
2019-2025
King Center
2021-2025
McGovern Institute for Brain Research
2025
Chinese Institute for Brain Research
2022-2024
Tonghua Normal University
2021
National Institute of Biological Sciences, Beijing
2013-2021
Ocean University of China
2021
Shandong University
2019
Dezhou University
2019
Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, lack of specific antibodies to mouse protein impairs study splicing, post-translational processing, shedding, and temporal spatial expression endogenous fibrocystin at cellular subcellular level. Here, we report using a knock-in strategy generate null Pkhd1 strain that expresses along with two SV5-Pk epitope tags engineered in-frame into third exon, immediately...
Microtubule-based kinesin motor proteins are crucial for intracellular transport, but their hyperactivation can be detrimental cellular functions. This study investigated the impact of a constitutively active ciliary mutant, OSM-3CA, on sensory cilia in C. elegans. Surprisingly, we found that OSM-3CA was absent from underwent disposal through membrane abscission at tips aberrant neurites. Neighboring glial cells engulf and eliminate released process depends engulfment receptor CED-1. Through...
Cilia build distinct subdomains with variable axonemal structures to perform diverse functions in cell motility and signaling. In sensory cilia across species, an axoneme differentiates longitudinally into a middle segment nine microtubule (MT) doublets distal MT singlets that extends from the A tubules of doublets. Here, we study differentiation Caenorhabditis elegans by analyzing flagellar inner junction protein FAP20 PCRG1 connect B Chlamydomonas . The nematode CFAP-20 is restricted...
Cilia are remarkable cellular devices that power cell motility and transduce extracellular signals. To assemble a cilium, cylindrical array of 9 doublet microtubules push out an extension the plasma membrane. Membrane tension regulates cilium formation; however, molecular pathways link mechanical stimuli to ciliogenesis unclear. Using genome editing, we introduced hereditary elliptocytosis (HE)- spinocerebellar ataxia (SCA)-associated mutations into Caenorhabditis elegans membrane skeletal...
Significance We study microvilli of Caenorhabditis elegans larvae and mouse intestinal tissues by combining high-pressure freezing, cryo-focused ion-beam milling, cryo-electron tomography, subtomogram averaging. find that many radial nanometer bristles, referred to as nanobristles, project from the lateral surface nematode microvilli. The C. nanobristles are 37.5 nm long. show nanobristle formation requires a protocadherin family protein, CDH-8, in . loss cdh-8 mutants slows down animal...
The tubulin tail is a key element for microtubule (MT) functionality, but the functional redundancy of genes complicates genetic determination their physiological functions. Here, we removed C-terminal five alpha- and four beta-tubulin in C. elegans genome. Sensory cilia typically exhibit an axoneme that longitudinally differentiates into middle segment with doublet MTs distal singlet MTs. However, excision alpha-tubulin tail, not resulted ectopic formation segments. Molecular dynamics...
Breast cancer is the most common female malignancy worldwide, however its molecular pathogenesis still needs in-depth investigation. Here we first revealed that olfactory receptor family 2, subfamily T, member 6 (OR2T6) was significantly over-expressed in breast tissues compared with normal tissues. OR2T6 expression tightly correlated higher TNM staging, positive lymph node metastasis, and associated poorer patients' overall disease-free survival. And enhanced proliferation, invasion,...
Autosomal-dominant polycystic kidney disease (ADPKD) is a systemic disorder affecting multiple organs that results in renal and extrarenal cysts. Patients with ADPKD may have genomic instability, making them more vulnerable to developing cancer. This study aimed investigate latent instability patients ADPKD, using single-cell gel electrophoresis (comet assay).The susceptibility of peripheral blood lymphocytes DNA damage induced by X-ray treatment (0.5 Gy) was tested 20 electrophoresis. The...
(Cell 172, 344–357.e1–e7; January 11, 2018) Our paper reported a mechanism where E. coli transcription-coupled repair factor Mfd utilizes DNA translocation to dynamically regulate transcription. We have identified three minor errors in the manuscript. The first error is located Results section, paragraph entitled “Mfd Translocates on Its Own.” sentence originally read: "If translocase moves toward fork, dsDNA unzipped, whereas if away from rezipped.” However, it should “If second text legend...
Apoptosis, a significant form of cell death, has leading role in the host defense against virus infection. Viruses have evolved series strategies that block apoptosis during early stage viral infection to enhance replication, and induce late stages facilitate particle release from cells. Here we show orf (ORFV), causative agent orf, encodes an apoptosis-inducing protein ORFV119. ORFV119 targets mitochondria cells, inhibits proliferation, induces apoptosis. Protein array data indicated could...
Background Because of the life‐consuming treatment and severe consequences associated with thalassemia, it is more effective to prevent than cure thalassemia. Rapid sensitive detection critical for controlling In this study, we developed a rapid accurate test genotype nondeletional α‐ β‐thalassemia mutations by an electrochemical DNA sensor. Methods Screen‐printed electrodes were used as transducers sensor, in which capture probe was attached golden surface working electrode via S–Au...
Poly(lactic acid) (PLA)/poly(ϵ-caprolactone) (PCL)/nano-silica composite degradable films were prepared by a solvent casting method. SEM results showed that the nano-silica particles dispersed uniformly in PLA/PCL matrix. TGA indicated thermal decomposition temperature rose with increase of content. The tensile strength composites was enhanced content up to 2%. increased silica and reached its maximum (22.51 Mpa). improvement water uptake ratio PLA/PCL/silica nanocomposites may be...
Heparan sulfate is a linear polysaccharide and serves as an important biomarker to monitor patient response therapies for MPS III disorder. It challenging analyze heparan intact owing its complexity heterogeneity. Therefore, sensitive, robust validated LC-MS/MS method needed support the clinical studies quantitation of in biofluids under regulated settings. Presented this work are results development validation human urine using selected high-abundant disaccharides surrogates. During sample...
Abstract Single-molecule visualization in cells with genetically encoded tags for electron microscopy (EM) has been a long-awaited but unimplemented tool cell biologists. Here, we report an approach directly synthesizing EM-visible gold nanoparticles (AuNPs) on cysteine-rich single-molecule cells. We first uncovered auto-nucleation suppression mechanism that allows specific synthesis of AuNPs isolated tags. next exploited this to develop detection proteins prokaryotic and achieved...